The paucity of information regarding antimicrobial sensitivity of microorganisms in diabetic foot infections in Libyan patients prompted undertaking the present study in 100 diabetic persons. Out of the total 100 samples, 212 bacteria were grown on different media for aerobic and anaerobic microbes. The poly-microbial cultures were significantly (63%) more common than mono-microbial (35%) with no growth in two samples. Out of all 212 bacteria grown, 148 aerobics were more than anaerobics (64) (p<0.05). The anti-microbial sensitivity testing reveals that staphylococci were sensitive to Augmentin (38%) and Gentamycin (40%); all of the Gram-negative bacilli were highly sensitive to Gentamycin with the exception of Kleibseilla. Proteus showed moderate sensitivity to Augmentin while this drug was highly effective (83%) against E. coli; Erythromycin (59%) was second to Gentamycin (76%) against Pseudomonas. Kleibseilla showed high sensitivity (67%) to tetracycline and Sulfamethoxazole. Enterobacter was highly sensitive (70%) to both Clindamycin and Trimethoprim. Citrobacter was the only sensitive bacterium (70%) to Gentamycin. Serratia showed some sensitivity (25%) to Gentamycin and Amoxicillin. In case of anaerobic bacteria, Clostridium perfringens were sensitive to Metronidazole (63%) and Cloxacitlin (30%); Bacteriodes showed moderate (50%) to Metronidazole and lower (30%) sensitivity to Cloxacillin. Pepto-streptococcus was resistant to all antimicrobials except Augmentin, Tetracycline and Metronidazole with 100% sensitivity to all.