تُعزز السقالة الحيوية تسهيل التصاق وتكاثر الخلايا، بالإضافة إلى انتشار عوامل النمو والعناصر الغذائية، وبهذا تؤمن تكاثر وتمايز الخلايا الجذعية أثناء عملية التجدُّد النسيجي. ويُقدم ببتيد هيدروجيل Puramatrix® (PM) ذا الألياف النانوية والقوام الهلامي سقالة هلامية مُميزة لدعم تكاثر الخلايا وهو يوفر بيئة صغرية مماثلة للمطرس خارج الخلوي في سياق الهندسة النسيجية.
هدفت هذه الدراسة إلى تقييم عيوشية الخلايا الشحمية العائدة عن التمايز والمأخوذة من النسيج الشحمي للكلب ضمن سقالة الببتيد هيدروجيل المُصنّعة (Puramatrix®) (دراسة في المختبر).
عُزلت الخلايا الشحمية العائدة عن التمايز (Dedifferintiated Fat Cells) DFAT من النسيج الشحمي للكلب بالهضم بالكولاجيناز والتنبيذ اللاحق. واستنبتت خلايا الإمرار الثاني ضمن أطباق 96 حجرة. وبعد 24 ساعة، أُضيفت ثلاثة تراكيز مختلفة من سقالة PM (0.15، 0.25، 0.5 %) إلى الحجر التجريبية. واعتمدت فترتان لتحري عيوشية الخلايا ضمن السقالة: 48 ساعة و7 أيام. وجرى تقييم عيوشية خلايا DFAT ضمن سقالة PM بمقايسة MTT.
أظهرت النتائج عدم وجود فارق احصائي هام بين التراكيز الثلاثة لسقالة PM ومع المجموعة الشاهدة بعد استنبات الخلايا DFAT ضمن سقالة PM لمدة 48 ساعة (0.563 =P). من ناحية أخرى، حسنت تراكيز PM الثلاثة، بعد 7 أيام، من عيوشية خلايا DFAT بشكل يُعتد به، مقارنةً مع المجموعة الشاهدة (P<0.001). حتى أن تركيزي (PM (0.15، 0.25% حققا عيوشية خلايا DFAT، مقارنة بالتركيز 0.5% بعد 7 أيام من الاستنبات(P<0.05).
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