استخدمت في هذه الدراسة طريقتنان لتعيين البروتين، طريقة كيلدال وطريقة ربط الصباغ باستخدام Orange G ومقياس الطيف الضوئي في موجة أعظمية طولها 478 نانومتر، بتعيين الخطية والمضبوطية والدقة وحد الكشف وحد القياس الكمي لكل منهما. وبالمقارنة، اختيرت طريقة ربط الصباغ لمضبوطيتها وتكراريتها وحساسيتها (حد الكشف، حد القياس الكمي) وسرعة إنجازها. وطُبّقت بعد ذلك، على عينات اللبن الرائب العادي واللبنة والعيران. تراوحت النتائج بين 3.41–3.88 غ/ 100غ للبن الرائب العادي، 5.48–11.48 غ/ 100غ للبنة، 1.48–2.22 غ/ 100غ للبن العيران.
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