تُعدّ النباتات مصدراً مهمّاً للعديد من المركبات الفينولية التي يعزى لها الفعالية المضادة للتأكسد وما لهذه الفعالية من دور في العديد من حالات العلاج والوقاية من السرطان.
أُجري هذا البحث لتحديد المحتوى الكلي من الفينولات والفعالية المضادة للتأكسد لنباتات دم الغزال والمصيص وزهرة الألماسة المنتشرة في سورية، ولاستقصاء اثرها على عيوشية الخلايا السرطانية، حيث جرى استخلاص مركبات النباتات بالميثانول باستخدام طريقة بسوكسليه. وحُددت الفعالية المضادة للتأكسد للخلاصات النباتية بتعيين محتواها من الفينولات الكلية، وقدرتها الكاسحة للجذر 2،2- ثنائي فينيل -1- بيكريل هيدرازيل (DPPH). ومن ثم ّدُرِس تأثير هذه الخلاصات على عيوشية خلايا سرطان الكبد البشري HepG2، بالمقارنة مع دواء معياري باستخدام كاشف XTT، مع ستة تراكيز لكل خلاصة نباتية ولفترات علاج مختلفة، 24، 48، 72 ساعة.
أظهرت النتائج أن قيمة المحتوى الكلي من الفينولات كانت أعلى في الخلاصة الميثانولية لنبات دم الغزال، ولم يكن هناك فارق ذو دلالة احصائية في الأثر الكاسح لجذر DPPH الحرّ للنباتات الثلاثة. كما أظهرت نتائج اختبار العيوشية كفاءة للخلاصات النباتية لزهرة الألماسة على خلايا HepG2 بعد 24h و48h، حيث وصلت IC50 إلى قيمة 224.55 µg/mL و14 µg/mL، على الترتيب، وبعد 72h للنباتات الثلاثة بلغت IC50 قيمة 6.708 µg/mL لنبات زهرة الألماسة و37.958 µg/mL لنبات دم الغزال و53.650 µg/mL لنبات المصيص.
1-William Charles Evans; Trease and Evans Pharmacognosy fifteenth edition. Printed and bound in Great Britain by the Bath Press, Bath, 15: 214, 2008.
2-Spiridon E.Kintzios and Maria G.Barberaki.Plants that Fight Cancer.CRC Press LLC, 23, 27, 2004.
3-George E. post, M.D, M.A, D.D.S. Flora Of Syria, Plasetine,And Sinai From the Tourusto Ramuhummedand From Mediteranean Sea To The Syrian Desert, The American press Beirut , Syria :656-658, 1990.
4-Nidal Jaradat; Mohammad Qneibi; Mohammed Hawash; Anood Sawalha; Sana Qtaishat; Fatima Hussein; Linda Issa. Chemical Composition, Antioxidant, Antiobesity and Antidiabetic Effects of Helichrysum sanguineum (L.) Kostel from Palestine.
Arabian Journal for Science and Engineering, 10. 3: 342-347, 2020.
5-Aslana M; Özçelik B; Orhana I; Karaoglu T; Sezika E. Screening of antibacterial, antifungal and antiviral properties of the selected Turkish Helichrysum species.
Planta Medicine, 72, 11: 45-72, 2006.
6-Erolu EH; Hamzaolu E; Aksoy A; Budak Ü; Özkul Y. In vitro genotoxic effects of four Helichrysum species in human lymphocytes cultures.
Biological Research, 43, 2: 177–182, 2010.
7-Mericli A; Cubukcu B; Dortunc T. Flavonoids and anthocyanins of Helichrysum Sanguineum. Fitoterapia, 55:112–115, 1984.
8-Nidal Jaradat; Motasem Al‑Masri; Abdel Naser Zaid; Fatima Hussein; Khalid Ahmad Shadid; Fuad Al‑Rimawi; Khaled Shayeb; Afnan Sbeih; Alia Eid. Assessment of the antimicrobial and free radical scavenging activities of Moluccella spinosa, Helichrysum sanguineum, and Styrax officinalis folkloric medicinal plants from Palestine.
Oriental Pharmacy and Experimental Medicine, 18. 1: 234-236, 2018.
9- Ammar Bader; Qasem M. A. Abdallah; Mohamed I. S. Abdelhadi; Nunziatina De Tommasi; Nicola Malafronte ; Usama Shaheen ; Majdi M. Bkhaitan; and Roberta Cotugno. Cytotoxicity of Some Plants of the Asteraceae Family: Antiproliferative Activity of Psiadia punctulata Root Sesquiterpenes.
A C G publication, Journal of Records of Natural Products. 18, 10:1-8,2018.
10-Albayrak, S.; Sagdic, O.; Aksoy, A.; Hamzaoglu, E.: Antimicrobial and antioxidant activities of Helichrysum species from the Mediterranean region of Turkey.
Asian Journal of Chemistry. 20, 4: 3143, 2008.
11-Qasem Abdalah; Ibrahim Al-deeb; Ammar Bader; Fayez Hamam; Kamel Saleh and Amin Abdualmajid. Anti-angiogenic activity of Middle East medicinal plants of the Lamiaceae family. Journal of Molecular Medicine Reports, 18; 2441-2448, 2018.
12-Mahmoud A. Al-Qudah; Safwan M. Obeidat; Ayman M. Saleh; Ahmad A. ElOqlah; Eman Al-Masaeed; Hala I. Al-Jaber; Sultan T. Abu Orabi. Volatile Components Analysis, Total Phenolic, Flavonoid Contents, and Antioxidant Activity of Phlomis Species Collected from Jordan.
Journal of Essential Oil-Bearing Plants, 21, 3; 383-584, 2018.
13-F.U. Afifi; B. Al-Khalidi; E. Khalil. Studies on the in vivo hypoglycemic activities of two medicinal plants used in the treatment of diabetes in Jordanian traditional medicine following intranasal administration.
Journal. Ethnopharmacol, 100: 314-318, 2005.
14-D. Zama, Z. Meraihi; S. Tebibel, W. Benayssa; F. Benayache; S. Benayache; A. J. Vlietinck, Chlorpyrifos-induced oxidative stress and tissue damage in the liver, kidney, brain and foetus in pregnant rats: The protective role of the butanolic extract of Paronychia argentea L.,Indian
Journal of. Pharmacology, 39 (2007): 145-150, 2007.
15-A.G. Al-Bakri; F.U. Affifi; Evaluation of antimicrobial activity of selected plant extracts by rapid XTT colorimetry and bacterial enumeration. Journal of Microbiol. Method, 68: 19-25, 2007.
16-C. Hansawasdi; J. Kawabata; T. Kasai; Alpha amylase inhibitors from Roselle Hibiscus sabdariffaLinn. Tea. Bioscience, Biotechnology, and Biochemistry, 64, 5: 1041-1043, 2000.
17-S. Saita,b; S. Hamri-Zeghichia; L. Boulekbache-Makhloufa; K. Madania; P. Rigouc; V. Brighentid, F. Pio Prenciped; S. Benvenutid; F. Pellatid. HPLC-UV/DAD and ESI-MSn analysis of flavonoids and antioxidant activity of an Algerian medicinal plant: Paronychia argentea Lam.
Journal of Pharmaceutical and Biomedical analysis, 15: 1-37, 2015.
18-Hasan Y. Muti; Suleiman Olimat. HPLC Method of Analysis for Determination and Standardization of Luteolin and Vanillic acid in Dry Extract of Paronychia argentea Lam.
Oriental Journal of Chemistry, 34, 6: 2721-2727, 2018.
19-Singleton L; Orther R. and Lamuela-Ravents R. Analysis of Total Phenols and Other Oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent.
Journal of Methods in enzymology. 299:152-178, 1999.
20-Helena Abramovic; Blaz Grobin; Natas a Poklar Ulrih and Blaz Cigic. Relevance and Standardization of In Vitro Antioxidant Assays: ABTS, DPPH, and Folin–Ciocalteu.
Journal of Chemistry, 18: 9, 2018.
21-Mahmoud A. Al-Qudaha; Ayman M. Salehb; Naif L. Alhawsawib; Hala I. Al-Jaberc; Syed A. Rizvid and Fatma U. Afifie. Composition, Antioxidant and Anticancer activities of the Essential Oil from Fresh and Air-Dried Aerial Parts of Pallenis spinosa.
Journal of Chemistry & Biodiversity, 14, 8: 561-562, 2017.
22-Iqbal Ahmad; Farrukh Aqil; and Mohammad Owais. Modern Phytomedicine, 107, Wiley-VCH, 2006.
23-Md. Nur Alam; Nusrat Jahan Bristi; Md.Rafiquzzanman. Review on invivo and invitro methods evalutionof antioxidant activity.
Saudi Pharmaceutical Journal, 21:145, 2013.
24-Ihsan Calis; Hasan Kirmizibekmez1; John A. Beutler; Ali A. D¨Onmez; Funda Nuray Yalc¸In1, Ekrem Kilic; Meral¨Ozalp; Peter R¨Uedi; Deniz Tasdem˙ir. Secondary Metabolites of Phlomis viscosa and Their Biological Activities.
Turky Journal Chemistry, 29: 71-81-2005.
25-Erhan H Ero lu; Ergin Hamzao lu; Ahmet Aksoy; Ümit Budak; And Yusuf Özkul. In Vitro Genotoxic Effects of Four Helichrysum Species in Human Lymphocytes Cultures.
Biological Research, 43: 177-182, 2010.
26-Iqbal Ahmad; Farrukh Aqil; and Mohammad Owais. Modern Phytomedicine. WILEY VCH, 3: 115, 2006.
27-Moufida Adjadj; Meriem Djarmouni. Study of the Antioxidant Property and Xanthine Oxidase Inhibitory Activity of Various Extracts from the Algerian Medicinal Plant Paronychia argentea L. Pharmacognosy Communications. 8, 1: 49-53, 2018.
28-S. Sait; S. Hamri-Zeghichi; L. Boulekbache-Makhlouf; K. Madani; P. Rigou; V. Brighenti; F. Pio Prencipe; S. Benvenuti; F. Pellati. HPLC-UV/DAD and ESI-MS analysis of flavonoids and antioxidant activity of an Algerian medicinal plant: Paronychia argentea Lam.
Journal of Pharmaceutical and Biomedical Analysis, 15, 5: 1-37, 2015.
29-Wilfred Vermerris; Palph Nicholson. Phenolic Compound Biochemistry. Printed by Springer, 1: 153-154, 2006.
30-Na Li; Ji-Hua Liu; Jian Zhang and Bo-Yang Yu. Comparative Evaluation of Cytotoxicity and Antioxidative Activity of 20 Flavonoids.
Journal of Agriculturl and Food Chemistry. 56, 3876–3883, 2008.
31-Dubravka Bigovića; Katarina Šavikina; Teodora Jankovića; Nebojša Menkovića; Gordana Zdunića; Tatjana Stanojkovićb and Zorica Djurićc. Antiradical and Cytotoxic Activity of Different Helichrysum plicatum Flower Extracts. Natural Product Communications, 6, 6: 819-822, 2011.
32-Juliana M. Soares; Bruna M. De Faria; Lucas M. Ascari; Soniza V. Alves-Leon3; Jorge M. De Souza3; Antonio G. Soares4; Yraima Cordeiro And Luciana F. Romão. Diosmin induces caspase-dependent apoptosis in human glioblastoma cells. Annals of the Brazilian Academy of Sciences, 91. 4: 1-16, 2019.
33-Qasem Abdallah; Ibrahim Al-Deeb; Ammar Bader; Fayez Hamam; Kamel Saleh and Amin Abdulmajid. Anti-Angiogenic Activity of Middle East Medicinal Plants of the Lamiaceae Family. Molecular Medicine Reports, 18: 2441-2448, 2018.
34-Vishnu Priya Veeraraghavan, Sardar Hussain, Janardhana Papayya Balakrishna, Surapaneni Krishna Mohan. Paronychia Argentea: A Critical Comprehensive Review on its Diverse Medicinal Potential and Future as Therapeutics. A Multifaceted
Journal in the field of Natural Products and Pharmacognosy, 12, 5: 1172-1179, 2020.