{"id":108,"date":"2023-01-01T11:20:20","date_gmt":"2023-01-01T11:20:20","guid":{"rendered":"https:\/\/pharmjournal.asscph.com\/?p=108"},"modified":"2024-07-19T14:14:12","modified_gmt":"2024-07-19T14:14:12","slug":"%d8%af%d8%b1%d8%a7%d8%b3%d8%a9-%d8%a7%d9%84%d8%b3%d9%85%d9%8a%d8%a9-%d8%a7%d9%84%d8%ac%d9%8a%d9%86%d9%8a%d8%a9-%d8%a7%d9%84%d8%aa%d9%8a-%d8%aa%d8%b3%d8%a8%d8%a8%d9%87%d8%a7-%d8%b5%d8%a8%d8%ba%d8%a7","status":"publish","type":"post","link":"https:\/\/pharmjournal.asscph.com\/?p=108","title":{"rendered":"\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u0633\u0645\u064a\u0629 \u0627\u0644\u062c\u064a\u0646\u064a\u0629 \u0627\u0644\u062a\u064a \u062a\u0633\u0628\u0628\u0647\u0627 \u0635\u0628\u063a\u0627\u062a \u0627\u0644\u0634\u0639\u0631 \u0627\u0644\u062f\u0627\u0626\u0645\u0629 \u0627\u0644\u062d\u0627\u0648\u064a\u0629 \u0639\u0644\u0649 \u0628\u0627\u0631\u0627\u0641\u064a\u0646\u064a\u0644\u064a\u0646 \u062f\u064a \u0623\u0645\u064a\u0646 \u0641\u064a \u0627\u0644\u0632\u062c\u0627\u062c"},"content":{"rendered":"

\u0627\u0644\u0645\u0642\u062f\u0645\u0629 Introduction:<\/strong><\/span><\/p>\n

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\u0627\u062e\u062a\u0628\u0627\u0631 Ames test [11] \u0625\u0644\u0649 \u0627\u0644\u062f\u0631\u0627\u0633\u0627\u062a \u0639\u0644\u0649 \u062d\u064a\u0648\u0627\u0646\u0627\u062a \u0627\u0644\u062a\u062c\u0631\u0628\u0629 \u0645\u0646 \u062e\u0644\u0627\u0644 \u0627\u0644\u062a\u0637\u0628\u064a\u0642 \u0627\u0644\u062c\u0644\u062f\u064a \u0623\u0648 \u062d\u062a\u0649 \u0627\u0644\u0647\u0636\u0645\u064a [12]\u00a0 \u0648\u0639\u0644\u0649 \u0639\u064a\u0646\u0627\u062a \u0628\u0634\u0631\u064a\u0629 \u0645\u062a\u0646\u0648\u0639\u0629 \u0645\u062b\u0644 \u0644\u0645\u0641\u0627\u0648\u064a\u0627\u062a \u0627\u0644\u062f\u0645 \u0627\u0644\u0645\u062d\u064a\u0637\u064a\u0629 \u0623\u0648 \u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0638\u0647\u0627\u0631\u0629 \u0627\u0644\u0628\u0648\u0644\u064a\u0629 \u0623\u0648 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0638\u0647\u0627\u0631\u064a\u0629 \u0644\u0645\u062e\u0627\u0637\u064a\u0629 \u0627\u0644\u0641\u0645 [13]\u2013[15] \u0644\u062a\u062e\u0644\u0635 \u0627\u0644\u0648\u0643\u0627\u0644\u0629 \u0627\u0644\u062f\u0648\u0644\u064a\u0629 \u0644\u0623\u0628\u062d\u0627\u062b \u0627\u0644\u0633\u0631\u0637\u0627\u0646The International Agency for Research on Cancer (IARC) \u0628\u0639\u062f \u0630\u0644\u0643 \u0625\u0644\u0649 \u0627\u0644\u0642\u0631\u0627\u0631 \u0627\u0644\u0646\u0647\u0627\u0626\u064a \u0648\u0627\u0644\u0630\u064a \u0630\u0643\u0631 \u0623\u0646 \u0635\u0628\u063a\u0627\u062a \u0627\u0644\u0634\u0639\u0631 \u0627\u0644\u062d\u0627\u0648\u064a\u0629 \u0639\u0644\u0649 \u0627\u0644\u0623\u0645\u064a\u0646\u0627\u062a \u0627\u0644\u0639\u0637\u0631\u064a\u0629 \u0645\u0646 \u0627\u0644\u0645\u0645\u0643\u0646 \u0623\u0646 \u062a\u0643\u0648\u0646 \u0645\u0633\u0631\u0637\u0646\u0629 \u0639\u0644\u0649 \u0627\u0644\u0639\u0627\u0645\u0644\u064a\u0646 \u0641\u064a \u0627\u0644\u0645\u0647\u0646\u0629 \u0628\u0627\u0644\u0639\u0648\u062f\u0629 \u0625\u0644\u0649 \u0645\u0627 \u0633\u0645\u062a\u0647 \u0623\u062f\u0644\u0629 \u0645\u062d\u062f\u0648\u062f\u0629 \u0645\u0646 \u0632\u064a\u0627\u062f\u0629 \u062e\u0637\u0648\u0631\u0629 \u0627\u0644\u0625\u0635\u0627\u0628\u0629 \u0628\u0633\u0631\u0637\u0627\u0646 \u0627\u0644\u0645\u062b\u0627\u0646\u0629 \u0627\u0633\u062a\u0646\u0627\u062f\u0627\u064b \u0625\u0644\u0649 \u062f\u0631\u0627\u0633\u0627\u062a \u0648\u0628\u0627\u0626\u064a\u0629\u060c \u0630\u0644\u0643 \u0623\u0646 \u0647\u0624\u0644\u0627\u0621 \u0627\u0644\u0639\u0627\u0645\u0644\u064a\u0646 \u0645\u0646 \u0627\u0644\u0645\u0645\u0643\u0646 \u0623\u0646\u0647\u0645 \u064a\u062a\u0639\u0631\u0636\u0648\u0646 \u0644\u0644\u0639\u062f\u064a\u062f \u0645\u0646 \u0639\u0648\u0627\u0645\u0644 \u0627\u0644\u062e\u0637\u0648\u0631\u0629 \u0623\u062b\u0646\u0627\u0621 \u0642\u064a\u0627\u0645\u0647\u0645 \u0628\u0639\u0645\u0644\u0647\u0645 \u0648\u0644\u064a\u0633 \u0641\u0642\u0637 \u0644\u0635\u0628\u063a\u0627\u062a \u0627\u0644\u0634\u0639\u0631 [16]\u060c \u0625\u0636\u0627\u0641\u0629 \u0625\u0644\u0649 \u0630\u0644\u0643 \u0641\u0625\u0646 \u0627\u0644\u0627\u062e\u062a\u0644\u0627\u0641 \u0641\u064a \u0627\u0644\u062e\u0648\u0627\u0635 \u0627\u0644\u0641\u064a\u0632\u064a\u0627\u0626\u064a\u0629 \u0648\u0627\u0644\u0643\u064a\u0645\u064a\u0627\u0626\u064a\u0629 \u0644\u0645\u0631\u0643\u0628\u0627\u062a \u0627\u0644\u0623\u0645\u064a\u0646 \u0627\u0644\u0639\u0637\u0631\u064a\u0629 \u0645\u0646\u0639\u062a \u0645\u0646 \u0627\u0644\u0648\u0635\u0648\u0644 \u0625\u0644\u0649 \u0639\u0644\u0627\u0642\u0629 \u062a\u0631\u0628\u0637 \u0627\u0644\u0628\u0646\u064a\u0629 \u0628\u0627\u0644\u062a\u0623\u062b\u064a\u0631 \u0648\u0647\u0630\u0627 \u064a\u0633\u0628\u0628 \u0627\u062e\u062a\u0644\u0627\u0641 \u0641\u064a \u0627\u0644\u0633\u0645\u064a\u0629 \u0627\u0644\u0646\u0627\u062a\u062c\u0629 [17]\u2013[19]\u060c \u0645\u0627 \u064a\u062a\u0637\u0644\u0628 \u0641\u062d\u0635 \u0643\u0644 \u0645\u0631\u0643\u0628 \u0639\u0644\u0649 \u062d\u062f\u0629 \u0644\u0644\u0648\u0635\u0648\u0644 \u0625\u0644\u0649 \u0627\u0644\u062a\u0642\u064a\u064a\u0645 \u0627\u0644\u0633\u0645\u064a \u0644\u0647\u060c \u0639\u0644\u0645\u0627 \u0623\u0646 \u062a\u0641\u0627\u0639\u0644 \u0645\u0643\u0648\u0646\u0627\u062a \u0627\u0644\u0635\u0628\u063a\u0629 \u0645\u0639 \u0628\u0639\u0636\u0647\u0627 \u064a\u0646\u062a\u062c \u0639\u0646\u0647\u0627 \u0645\u0631\u0643\u0628\u0627\u062a \u062c\u062f\u064a\u062f\u0629 \u0644\u0647\u0627 \u0635\u064a\u063a\u0629 \u0627\u0644\u0623\u0645\u064a\u0646 \u0627\u0644\u0639\u0637\u0631\u064a \u0648\u0627\u0644\u062a\u064a \u062a\u0645\u0644\u0643 \u062e\u0648\u0627\u0635 \u0645\u062e\u062a\u0644\u0641\u0629 \u064a\u062a\u0648\u0642\u0641 \u062a\u0642\u064a\u064a\u0645\u0647\u0627 \u0639\u0644\u0649 \u0627\u0644\u0627\u062e\u062a\u0628\u0627\u0631\u0627\u062a \u0627\u0644\u062a\u064a \u0623\u062c\u0631\u064a\u062a \u0639\u0644\u064a\u0647\u0627. <\/span>\u0647\u0646\u0627\u0644\u0643 \u0627\u0644\u0639\u062f\u064a\u062f \u0645\u0646 \u0627\u0644\u0645\u0642\u0627\u064a\u0633\u0627\u062a \u0627\u0644\u062a\u064a \u062a\u0642\u064a\u0645 \u0627\u0644\u0633\u0645\u064a\u0629 \u0627\u0644\u062c\u064a\u0646\u064a\u0629 \u0644\u0644\u0645\u0648\u0627\u062f \u0627\u0644\u063a\u0631\u064a\u0628\u0629 \u0627\u0644\u062f\u0627\u062e\u0644\u0629 \u0625\u0644\u0649 \u0627\u0644\u0639\u0636\u0648\u064a\u0629 \u0627\u0644\u062d\u064a\u0629 \u0648\u0645\u0646 \u0628\u064a\u0646\u0647\u0627 \u0627\u062e\u062a\u0628\u0627\u0631 \u0627\u0644\u0646\u0648\u064a\u0629 \u0627\u0644\u0635\u063a\u064a\u0631\u0629\u00a0 \u0648\u0627\u0644\u062a\u064a \u062a\u0645 \u062a\u0636\u0645\u064a\u0646\u0647\u0627 \u0641\u064a \u00a0\u0645\u0646\u0638\u0645\u0629 \u0627\u0644\u062a\u0639\u0627\u0648\u0646 \u0627\u0644\u0627\u0642\u062a\u0635\u0627\u062f\u064a \u0648\u0627\u0644\u062a\u0646\u0645\u064a\u0629 Organization for Economic Co-operation and Development \u00a0(OECD) \u0644\u0645\u0642\u0627\u064a\u0633\u0629 \u0627\u0644\u0633\u0645\u064a\u0629 \u0627\u0644\u062c\u064a\u0646\u064a\u0629 \u0641\u064a \u0627\u0644\u0632\u062c\u0627\u062c \u0644\u0644\u062a\u0646\u0628\u0624 \u0628\u062e\u0637\u0648\u0631\u0629 \u0627\u0644\u0645\u0631\u0643\u0628\u0627\u062a \u0648\u0644\u062c\u0645\u0639 \u0645\u0639\u0644\u0648\u0645\u0627\u062a \u062d\u0648\u0644 \u0633\u0644\u0627\u0645\u062a\u0647\u0627\u060c \u062d\u064a\u062b \u062a\u0645\u062b\u0644 \u0647\u0630\u0647 \u0627\u0644\u0645\u0642\u0627\u064a\u0633\u0629 \u0623\u062f\u0627\u0629 \u062f\u0642\u064a\u0642\u0629 \u0648\u0641\u0639\u0627\u0644\u0629 \u0644\u0644\u062a\u062d\u0631\u064a \u0639\u0646 \u0634\u0630\u0648\u0630\u0627\u062a \u0627\u0644\u0635\u0628\u063a\u064a\u0627\u062a \u0627\u0644\u062a\u064a \u064a\u0645\u0643\u0646 \u0623\u0646 \u062a\u0646\u062a\u062c \u0639\u0646 \u0627\u0644\u0645\u0639\u0627\u0644\u062c\u0629 \u0628\u0645\u0648\u0627\u062f \u0630\u0627\u062a \u0633\u0645\u064a\u0629 \u062c\u064a\u0646\u064a\u0629 \u0645\u062d\u062a\u0645\u0644\u0629 \u0643\u0645\u0631\u0643\u0628 PPD [20]. <\/span>\u0625\u0646 \u0623\u0647\u0645 \u0627\u0644\u0637\u0631\u0642 \u0627\u0644\u062a\u064a \u064a\u062a\u0645 \u0645\u0646 \u062e\u0644\u0627\u0644\u0647\u0627 \u0627\u0644\u062a\u0639\u0631\u0636 \u0644\u0635\u0628\u063a\u0627\u062a \u0627\u0644\u0634\u0639\u0631 \u0647\u0648 \u0627\u0644\u0637\u0631\u064a\u0642 \u0627\u0644\u062c\u0644\u062f\u064a \u0639\u0646\u062f \u062d\u062f\u0648\u062b \u0627\u0644\u0627\u0645\u062a\u0635\u0627\u0635 \u0623\u062b\u0646\u0627\u0621 \u0627\u0644\u062a\u0637\u0628\u064a\u0642\u060c \u062d\u064a\u062b \u064a\u062d\u062f\u062b \u062a\u0639\u0631\u0636 \u0625\u0644\u0649 \u0646\u0648\u0627\u062a\u062c \u062a\u0641\u0627\u0639\u0644 \u0645\u0643\u0648\u0646\u0627\u062a \u0627\u0644\u0635\u0628\u063a\u0629\u00a0 [21]\u060c \u0644\u0630\u0644\u0643 \u0647\u062f\u0641\u062a \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0625\u0644\u0649 \u0627\u0644\u062a\u062d\u0631\u064a \u0639\u0646 \u0627\u0644\u0633\u0645\u064a\u0629 \u0627\u0644\u062c\u064a\u0646\u064a\u0629 \u0644\u0644\u062c\u0632\u0621 \u0627\u0644\u0645\u0645\u062a\u0635 \u0645\u0646 \u0635\u0628\u063a\u0629 \u0627\u0644\u0634\u0639\u0631 \u0627\u0644\u062f\u0627\u0626\u0645\u0629 \u0627\u0644\u062d\u0627\u0648\u064a\u0629 \u0639\u0644\u0649 \u0645\u0631\u0643\u0628 \u0627\u0644\u0628\u0627\u0631\u0627\u0641\u064a\u0646\u064a\u0644\u064a\u0646 \u062f\u064a \u0623\u0645\u064a\u0646 \u0639\u0644\u0649 \u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u062e\u0637 \u0627\u0644\u062e\u0644\u0648\u064a HEK293T \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u062e\u062a\u0628\u0627\u0631 \u0627\u0644\u0646\u0648\u064a\u0629 \u0627\u0644\u0635\u063a\u064a\u0631\u0629.<\/span><\/p>\n

\u0627\u0644\u0645\u0648\u0627\u062f \u0648\u0627\u0644\u0637\u0631\u0627\u0626\u0642<\/strong> Material and Methods <\/strong><\/span><\/p>\n

\u00a0 1- \u0627\u0644\u0645\u0648\u0627\u062f \u0627\u0644\u0645\u0633\u062a\u062e\u062f\u0645\u0629<\/strong><\/span><\/p>\n

\u0648\u0633\u0637 \u0627\u0644\u0627\u0633\u062a\u0646\u0628\u0627\u062a \u0627\u0644\u062e\u0644\u0648\u064a Dulbecco’s Modified Eagle Medium \u00a0(DMEM ) \u0648\u0645\u0635\u0644 \u0627\u0644\u062c\u0646\u064a\u0646 \u0627\u0644\u0628\u0642\u0631\u064a fetal bovine serum(FBS) \u0645\u0646\u0632\u0648\u0639 \u0627\u0644\u0645\u062a\u0645\u0645\u0629 \u0648\u0635\u0627\u062f\u0627\u062a \u062d\u064a\u0648\u064a\u0629 \u0645\u0646 \u0628\u0646\u0633\u0644\u0644\u064a\u0646 \u0648\u0633\u062a\u0631\u0628\u062a\u0648\u0645\u0627\u064a\u0633\u064a\u0646penicillin\/streptomycin \u060c \u0645\u062d\u0644\u0648\u0644 \u0648\u0642\u0627\u0621 \u0627\u0644\u0641\u0648\u0633\u0641\u0627\u062a \u0627\u0644\u062e\u0627\u0644\u064a \u0645\u0646 \u0627\u0644\u0645\u063a\u0646\u0632\u064a\u0648\u0645 \u0648\u0627\u0644\u0643\u0627\u0644\u0633\u064a\u0648\u0645 Phosphate-buffered saline (PBS) (\u0634\u0631\u0643\u0629 \u0628\u064a\u0648\u0633\u064a\u0631\u0627 Biosera INC\u060c \u0627\u0644\u0641\u064a\u0644\u064a\u0628\u064a\u0646Philippines)\u060c \u0648\u0645\u062d\u0644\u0648\u0644 \u0623\u0632\u0631\u0642 \u0627\u0644\u062a\u0631\u064a\u0628\u0627\u0646 Trypan Blue \u00a00.5% (\u0634\u0631\u0643\u0629 \u0633\u064a\u063a\u0645\u0627Sigma \u060c \u0627\u0644\u0648\u0644\u0627\u064a\u0627\u062a \u0627\u0644\u0645\u062a\u062d\u062f\u0629 \u0627\u0644\u0623\u0645\u0631\u064a\u0643\u064a\u0629United States )\u060c \u0648\u0627\u0644\u0645\u064a\u062a\u0627\u0646\u0648\u0644 Methanol 99.9% \u00a0(\u0634\u0631\u0643\u0629 PanReac AppliChem ITW Reagents\u060c \u0627\u0633\u0628\u0627\u0646\u064a\u0627 Spain)\u060c \u0648\u0645\u0644\u0648\u0646 \u063a\u064a\u0645\u0632\u0627 Giemsa”s solution (\u0634\u0631\u0643\u0629 SCP SCP Group\u060c \u0645\u0627\u0644\u064a\u0632\u064a\u0627).<\/span><\/p>\n

\"\"<\/span><\/p>\n

2- \u0627\u0644\u0645\u0648\u0627\u062f \u0627\u0644\u0645\u062f\u0631\u0648\u0633\u0629<\/strong><\/span><\/p>\n

\u0623\u0631\u0628\u0639 \u0635\u0628\u063a\u0627\u062a \u0634\u0639\u0631 \u062f\u0627\u0626\u0645\u0629 \u062a\u062d\u0648\u064a \u0639\u0644\u0649 \u0627\u0644\u0628\u0627\u0631\u0627\u0641\u064a\u0646\u064a\u0644\u064a\u0646 \u062f\u064a \u0623\u0645\u064a\u0646 \u0643\u0639\u0627\u0645\u0644 \u0637\u0644\u064a\u0639\u0629 \u0623\u0633\u0627\u0633\u064a \u0648\u0628\u0627\u0631\u0627 \u0623\u0645\u064a\u0646\u0648 \u0641\u064a\u0646\u0648\u0644 \u0643\u0639\u0627\u0645\u0644 \u0637\u0644\u064a\u0639\u0629 \u0625\u0636\u0627\u0641\u064a \u064a\u062f\u062e\u0644 \u0641\u064a \u062a\u0631\u0643\u064a\u0628 \u0628\u0639\u0636 \u0627\u0644\u0635\u0628\u063a\u0627\u062a \u0645\u0639 \u0648\u0627\u062d\u062f \u0623\u0648 \u0623\u0643\u062b\u0631 \u0645\u0646 \u0627\u0644\u0639\u0648\u0627\u0645\u0644 \u0627\u0644\u0631\u0627\u0628\u0637\u0629 \u062a\u0645 \u0630\u0643\u0631\u0647\u0627 \u0641\u064a \u0627\u0644\u062c\u062f\u0648\u0644 (1). \u0639\u0644\u0645\u0627\u064b \u0628\u0623\u0646 \u0627\u0644\u0635\u064a\u063a\u0629 (1) \u062a\u0639\u0637\u064a \u0627\u0644\u0644\u0648\u0646 \u0623\u0634\u0642\u0631 \u0648\u0627\u0644\u0635\u064a\u063a\u0629 (2) \u062a\u0639\u0637\u064a \u0627\u0644\u0644\u0648\u0646 \u0627\u0644\u0623\u0633\u0648\u062f \u0648\u0627\u0644\u0635\u064a\u063a\u0629 (3) \u062a\u0639\u0637\u064a \u0627\u0644\u0644\u0648\u0646 \u0627\u0644\u0623\u062d\u0645\u0631 \u0648\u0627\u0644\u0635\u064a\u063a\u0629 (4) \u062a\u0639\u0637\u064a \u0627\u0644\u0644\u0648\u0646 \u0627\u0644\u0628\u0646\u064a.<\/span><\/p>\n

3<\/strong>–<\/strong> \u0627\u0644\u062e\u0637 \u0627\u0644\u062e\u0644\u0648\u064a: <\/strong><\/span><\/p>\n

\u062a\u0645 \u0627\u0644\u0639\u0645\u0644 \u0639\u0644\u0649 \u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u062e\u0637 \u0627\u0644\u062e\u0644\u0648\u064a HEK293T \u0627\u0644\u0645\u0634\u062a\u0642 \u0645\u0646 \u0643\u0644\u064a\u0629 \u062c\u0646\u064a\u0646 \u0628\u0634\u0631\u064a\u060c \u0648\u0627\u0644\u062a\u064a \u062a\u0645 \u0627\u0633\u062a\u0646\u0628\u0627\u062a\u0647\u0627 \u0641\u064a \u0648\u0633\u0637 \u0645\u0646 DMEM \u0627\u0644\u0645\u0636\u0627\u0641 \u0644\u0647 \u0645\u0635\u0644 \u062c\u0646\u064a\u0646 \u0628\u0642\u0631\u064aFBS \u0628\u0646\u0633\u0628\u0629 (10%) \u0648\u0635\u0627\u062f\u0627\u062a \u062d\u064a\u0648\u064a\u0629 \u0628\u0646\u0633\u0628\u0629 (1%) \u0648\u0630\u0644\u0643 \u0636\u0645\u0646 \u062d\u0627\u0636\u0646\u0629 \u062a\u0624\u0645\u0646 \u062f\u0631\u062c\u0629 \u062d\u0631\u0627\u0631\u0629 \u062a\u0635\u0644 \u0625\u0644\u0649 37 \u062f\u0631\u062c\u0629 \u0645\u0626\u0648\u064a\u0629 \u0648\u063a\u0627\u0632 \u062b\u0646\u0627\u0626\u064a \u0623\u0643\u0633\u064a\u062f \u0627\u0644\u0643\u0631\u0628\u0648\u0646 \u0628\u0646\u0633\u0628\u0629 (5%).<\/span><\/p>\n

4- \u0645\u062d\u0627\u0644\u064a\u0644 \u0627\u0644\u0635\u0628\u063a\u0627\u062a \u0627\u0644\u0645\u0633\u062a\u062e\u062f\u0645\u0629 \u0644\u0644\u0645\u0639\u0627\u0644\u062c\u0629:<\/strong><\/span><\/p>\n

\u062a\u0645 \u062a\u062d\u0636\u064a\u0631\u0647\u0627 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062e\u0644\u064a\u0629 \u0641\u0631\u0627\u0646\u0632Franz \u0645\u062d\u0644\u064a\u0629 \u0627\u0644\u0635\u0646\u0639 [22] \u0644\u0644\u062d\u0635\u0648\u0644 \u0639\u0644\u0649 \u0627\u0644\u062c\u0632\u0621 \u0627\u0644\u0645\u0645\u062a\u0635 \u0645\u0646 \u0627\u0644\u0635\u0628\u063a\u0629\u060c \u0648\u0630\u0644\u0643 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u063a\u0634\u0627\u0621 \u0637\u0628\u064a\u0639\u064a \u0645\u0646 \u062c\u0644\u062f \u0627\u0644\u062c\u0631\u0630 \u0648\u0648\u0636\u0639 \u0648\u0633\u0637 \u0627\u0644\u0627\u0633\u062a\u0646\u0628\u0627\u062a DMEM \u0641\u064a \u062d\u062c\u0631\u0629 \u0627\u0644\u0627\u0633\u062a\u0642\u0628\u0627\u0644 \u0644\u0644\u062e\u0644\u064a\u0629. \u062d\u064a\u062b \u0637\u064f\u0628\u0642\u062a \u0643\u0644 \u0645\u0646 \u0635\u0628\u063a\u0627\u062a \u0627\u0644\u0634\u0639\u0631 \u0639\u0644\u0649 \u0627\u0644\u063a\u0634\u0627\u0621 \u0645\u062f\u0629 45 \u062f\u0642\u064a\u0642\u0629 \u0648\u062a\u0631\u0643 \u0627\u0644\u063a\u0634\u0627\u0621 \u0639\u0644\u0649 \u0627\u0644\u062e\u0644\u064a\u0629 \u0645\u062f\u0629 24 \u0633\u0627\u0639\u0629 \u0628\u0639\u062f \u0625\u0632\u0627\u0644\u0629 \u0627\u0644\u0635\u0628\u063a\u0629\u060c \u0644\u064a\u0631\u0634\u062d \u0628\u0639\u062f \u0630\u0644\u0643 \u0627\u0644\u0645\u062d\u0644\u0648\u0644 \u0627\u0644\u0645\u0633\u062a\u0642\u0628\u0644 \u0628\u0641\u0644\u0627\u062a\u0631 \u062c\u0631\u062b\u0648\u0645\u064a\u0629 (0.2 \u0645\u064a\u0643\u0631\u0648\u0646) \u0648\u064a\u064f\u062c\u0645\u0639 \u0641\u064a \u0639\u0628\u0648\u0627\u062a \u0639\u0642\u064a\u0645\u0629 \u0648\u064a\u062d\u0641\u0638 \u0644\u062d\u064a\u0646 \u0627\u0644\u0645\u0642\u0627\u064a\u0633\u0629 \u0628\u062f\u0631\u062c\u0629 \u062d\u0631\u0627\u0631\u0629 -20 \u062f\u0631\u062c\u0629 \u0645\u0626\u0648\u064a\u0629. \u062d\u064f\u0636\u0631\u062a \u0623\u0631\u0628\u0639\u0629 \u062a\u0631\u0627\u0643\u064a\u0632 \u0644\u0645\u0639\u0627\u0644\u062c\u0629 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0648\u0647\u064a\u00b5L\/100\u00b5L \u00a02.5,10,25,50 \u0645\u0646 \u0645\u062d\u0644\u0648\u0644 \u0627\u0644\u0635\u0628\u063a\u0629 \u0646\u0633\u0628\u0629 \u0625\u0644\u0649 \u0648\u0633\u0637 \u0627\u0644\u0627\u0633\u062a\u0646\u0628\u0627\u062a .<\/span><\/p>\n

5- \u0627\u062e\u062a\u0628\u0627\u0631 \u0627\u0644\u0646\u0648\u064a\u0629 \u0627\u0644\u0635\u063a\u064a\u0631\u0629 <\/strong>\u00a0Micronucleus test (MN) <\/strong><\/span><\/p>\n

\u064a\u0642\u0648\u0645 \u0647\u0630\u0627 \u0627\u0644\u0627\u062e\u062a\u0628\u0627\u0631 \u0639\u0644\u0649 \u062d\u0636\u0646 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0645\u0639\u0627\u0644\u062c\u0629 \u0628\u0627\u0644\u0645\u0627\u062f\u0629 \u0627\u0644\u0645\u062f\u0631\u0648\u0633\u0629 \u0641\u062a\u0631\u0629 \u0632\u0645\u0646\u064a\u0629 \u062a\u0639\u0627\u062f\u0644 1.5 \u0627\u0644\u0641\u062a\u0631\u0629 \u0627\u0644\u0632\u0645\u0646\u064a\u0629 \u0627\u0644\u0644\u0627\u0632\u0645\u0629 \u0644\u0625\u062a\u0645\u0627\u0645 \u0627\u0644\u062f\u0648\u0631\u0629 \u0627\u0644\u062e\u0644\u0648\u064a\u0629 \u0627\u0644\u0637\u0628\u064a\u0639\u064a\u0629 \u062b\u0645 \u062a\u062b\u0628\u064a\u062a \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0648\u062a\u0644\u0648\u064a\u0646\u0647\u0627 \u0628\u0645\u0644\u0648\u0646 \u0645\u062a\u0623\u0644\u0642 \u0648\u0645\u0646 \u062b\u0645 \u062a\u0633\u062c\u064a\u0644 \u0639\u062f\u062f \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u062d\u0627\u0648\u064a\u0629 \u0639\u0644\u0649 \u0646\u0648\u064a\u0629 \u0635\u063a\u064a\u0631\u0629 \u0645\u0646 \u0623\u0635\u0644 1000 \u062e\u0644\u064a\u0629 \u0623\u062d\u0627\u062f\u064a\u0629 \u0627\u0644\u0646\u0648\u0627\u0629 \u00a0mononucleate cells \u0648\u062b\u0646\u0627\u0626\u064a\u0629 \u0627\u0644\u0646\u0648\u0627\u0629 binucleate cells\u00a0 [23]. \u0625\u0646 \u0647\u0630 \u0627\u0644\u0627\u062e\u062a\u0628\u0627\u0631 \u064a\u062a\u0645\u062a\u0639 \u0628\u062f\u0642\u0629 \u0648\u0641\u0639\u0627\u0644\u064a\u0629 \u0641\u064a \u0627\u0644\u062a\u062d\u0631\u064a \u0639\u0646 \u0634\u0630\u0648\u0630\u0627\u062a \u0627\u0644\u0635\u0628\u063a\u064a\u0627\u062a \u0628\u0645\u0648\u062b\u0648\u0642\u064a\u0629 \u0639\u0627\u0644\u064a\u0629 \u0648\u0647\u0648 \u0643\u0645\u0627 \u0623\u0634\u0627\u0631\u062a OECD \u064a\u0645\u062b\u0644 \u0623\u062f\u0627\u0629 \u062a\u062d\u0631\u064a \u0633\u0647\u0644\u0629 \u0648\u062d\u0633\u0627\u0633\u0629 \u0644\u0644\u062a\u0646\u0628\u0624 \u0639\u0646 \u062a\u0639\u0631\u0636 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0644\u0645\u0627\u062f\u0629 \u0645\u0637\u0641\u0631\u0629. <\/span>\u0623\u064f\u0646\u062c\u0632 \u0647\u0630\u0627 \u0627\u0644\u0627\u062e\u062a\u0628\u0627\u0631 \u0628\u0627\u0644\u0627\u0639\u062a\u0645\u0627\u062f \u0639\u0644\u0649 \u0628\u0631\u0648\u062a\u0648\u0643\u0648\u0644 OECD487 [24] \u00a0\u0645\u0639 \u0628\u0639\u0636 \u0627\u0644\u062a\u0639\u062f\u064a\u0644\u0627\u062a. \u062d\u064a\u062b \u062a\u0645\u064b \u0627\u0633\u062a\u0646\u0628\u0627\u062a \u062e\u0644\u0627\u064a\u0627 HEK293T \u0641\u064a \u0635\u0641\u064a\u062d\u0629 6 \u0622\u0628\u0627\u0631 \u0628\u062d\u064a\u062b \u0648\u0636\u0639 0.3\u00d7106<\/sup> \u062e\u0644\u064a\u0629 \u0641\u064a \u0643\u0644 \u0628\u0626\u0631 \u0648\u0643\u0627\u0646 \u0627\u0644\u062d\u062c\u0645 \u0627\u0644\u0646\u0647\u0627\u0626\u064a \u0644\u0644\u0628\u0626\u0631 \u0647\u0648 2ml \u0645\u0646 \u0648\u0633\u0637 \u0627\u0644\u0627\u0633\u062a\u0646\u0628\u0627\u062a DMEM \u0627\u0644\u0645\u062f\u0639\u0645 \u0628\u0645\u0635\u0644 \u0627\u0644\u062c\u0646\u064a\u0646 \u0627\u0644\u0628\u0642\u0631\u064a FBS \u0648\u062d\u0636\u0646\u062a \u0627\u0644\u0635\u0641\u064a\u062d\u0629 24 \u0633\u0627\u0639\u0629. \u0639\u0648\u0644\u062c\u062a \u0628\u0639\u062f \u0630\u0644\u0643 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0628\u0627\u0644\u062a\u0631\u0627\u0643\u064a\u0632 \u0627\u0644\u0645\u064f\u062d\u0636\u0631\u0629 \u0633\u0627\u0628\u0642\u0627\u064b \u0645\u0646 \u0645\u062d\u0627\u0644\u064a\u0644 \u0635\u0628\u063a\u0627\u062a \u0627\u0644\u0634\u0639\u0631 \u0645\u062f\u0629 45 \u0633\u0627\u0639\u0629 \u0641\u064a \u062d\u0627\u0636\u0646\u0629 CO2 \u0648\u0628\u062f\u0631\u062c\u0629 \u062d\u0631\u0627\u0631\u0629 37 \u062f\u0631\u062c\u0629 \u0645\u0626\u0648\u064a\u0629 \u0645\u0639 \u0634\u0627\u0647\u062f \u0633\u0644\u0628\u064a \u0639\u0628\u0627\u0631\u0629 \u0639\u0646 \u062e\u0644\u0627\u064a\u0627 \u0645\u0632\u0631\u0648\u0639\u0629 \u0628\u0646\u0641\u0633 \u0627\u0644\u0639\u062f\u062f \u0641\u064a \u0648\u0633\u0637 \u0627\u0644\u0627\u0633\u062a\u0646\u0628\u0627\u062a \u0648\u0628\u062f\u0648\u0646 \u0623\u064a \u0645\u0639\u0627\u0644\u062c\u0629 \u0648\u0634\u0627\u0647\u062f \u0625\u064a\u062c\u0627\u0628\u064a \u0639\u0628\u0627\u0631\u0629 \u0639\u0646 \u062e\u0644\u0627\u064a\u0627 \u0645\u0632\u0631\u0648\u0639\u0629 \u0628\u0646\u0641\u0633 \u0627\u0644\u0639\u062f\u062f \u0648\u0645\u0639\u0627\u0644\u062c\u0629 \u0628\u0627\u0644\u0643\u0648\u0644\u0634\u0633\u064a\u0646 \u0628\u062a\u0631\u0643\u064a\u0632 5\u00b5g\/ml. \u0644\u064a\u062c\u0631\u064a \u0628\u0639\u062f\u0647\u0627 \u0641\u0643 \u0627\u0644\u062a\u0635\u0627\u0642 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0648\u063a\u0633\u0644\u0647\u0627 \u0628\u0648\u0642\u0627\u0621 \u0627\u0644\u0641\u0648\u0633\u0641\u0627\u062a \u062b\u0645 \u062a\u0646\u0628\u064a\u0630\u0647\u0627 \u0628\u0633\u0631\u0639\u0629 790rpm \u0645\u062f\u0629 \u062b\u0644\u0627\u062b \u062f\u0642\u0627\u0626\u0642 \u0648\u0625\u0632\u0627\u0644\u0629 \u0627\u0644\u0637\u0627\u0641\u064a. \u062b\u064f\u0628\u062a\u062a \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0639\u0644\u0649 \u0634\u0631\u0627\u0626\u062d \u0632\u062c\u0627\u062c\u064a\u0629 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0645\u064a\u062a\u0627\u0646\u0648\u0644 99.9% \u0648\u062a\u064f\u0631\u0643\u062a \u062d\u062a\u0649 \u062a\u062c\u0641 \u0628\u062f\u0631\u062c\u0629 \u062d\u0631\u0627\u0631\u0629 \u0627\u0644\u063a\u0631\u0641\u0629 \u062b\u0645 \u0635\u064f\u0628\u063a\u062a \u0628\u0645\u0644\u0648\u0646 \u063a\u064a\u0645\u0632\u0627 \u0628\u062a\u0631\u0643\u064a\u0632 4% \u0645\u062f\u0629 25 \u062f\u0642\u064a\u0642\u0629 \u062b\u0645 \u063a\u064f\u0633\u0644\u062a \u0628\u0627\u0644\u0645\u0627\u0621 \u0627\u0644\u062c\u0627\u0631\u064a \u0648\u062a\u064c\u0631\u0643\u062a \u062d\u062a\u0649 \u062a\u062c\u0641 \u0628\u0627\u0644\u0647\u0648\u0627\u0621. \u0648\u0623\u062e\u064a\u0631\u0627\u064b \u0641\u064f\u062d\u0635\u062a \u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u0627\u0644\u0645\u062c\u0647\u0631 \u0627\u0644\u0639\u0627\u062f\u064a (Olympus \u060c \u0627\u0644\u064a\u0627\u0628\u0627\u0646Japan )\u00a0 \u0628\u062a\u0643\u0628\u064a\u0631\u00d7100 \u0648\u062d\u0633\u0628 \u0639\u062f\u062f \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u062a\u064a \u062a\u062d\u062a\u0648\u064a \u0639\u0644\u0649 \u0627\u0644\u0646\u0648\u064a\u0629 \u0627\u0644\u0635\u063a\u064a\u0631\u0629 \u0643\u0645\u0627 \u064a\u0638\u0647\u0631 \u0641\u064a \u0627\u0644\u0634\u0643\u0644 (2) \u0628\u0639\u062f \u0641\u062d\u0635 1000 \u062e\u0644\u064a\u0629 \u0648\u0639\u064f\u0631\u0636\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0643\u0646\u0633\u0628 \u0645\u0626\u0648\u064a\u0629.<\/span><\/p>\n

\"\u0627\u0644\u0634\u0643\u0644
\u0627\u0644\u0634\u0643\u0644 (2) \u062e\u0644\u0627\u064a\u0627 HEK293T \u0627\u0644\u062d\u0627\u0648\u064a\u0629 \u0639\u0644\u0649 \u0646\u0648\u064a\u0629 \u0635\u063a\u064a\u0631\u0629<\/span><\/strong><\/figcaption><\/figure>\n

\u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u0625\u062d\u0635\u0627\u0626\u064a\u0629<\/strong> Statistical study <\/strong><\/span><\/p>\n

\u062a\u0645 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0639\u0646 \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0645\u062a\u0648\u0633\u0637\u0627\u062a \u00b1 \u0627\u0644\u0627\u0646\u062d\u0631\u0627\u0641 \u0627\u0644\u0645\u0639\u064a\u0627\u0631\u064aStandard deviation ((SD\u060c \u0648\u062f\u0631\u0633\u062a \u0627\u0644\u0641\u0631\u0648\u0642 \u0627\u0644\u0645\u0639\u0646\u0648\u064a\u0629 \u0644\u0644\u0645\u0642\u0627\u0631\u0646\u0629 \u0628\u064a\u0646 \u0623\u0643\u062b\u0631 \u0645\u0646 \u0645\u062c\u0645\u0648\u0639\u062a\u064a\u0646 \u0641\u064a \u0627\u062e\u062a\u0628\u0627\u0631 \u0627\u0644\u0646\u0648\u064a\u0629 \u0627\u0644\u0635\u063a\u064a\u0631\u0629 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 one way ANOVA \u0648\u0630\u0644\u0643 \u0639\u0646\u062f \u0645\u0633\u062a\u0648\u0649 \u062f\u0644\u0627\u0644\u0629 P <0.05 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0628\u0631\u0646\u0627\u0645\u062c \u0627\u0644\u062a\u062d\u0644\u064a\u0644 \u0627\u0644\u0625\u062d\u0635\u0627\u0626\u064aGraphPad \u00a0\u0627\u0644\u0625\u0635\u062f\u0627\u0631 8.0.<\/span><\/p>\n

\u0627\u0644\u0646\u062a\u0627\u0626\u062c <\/strong>Results<\/strong><\/span><\/p>\n

\u0625\u0646 \u062a\u0635\u0645\u064a\u0645 \u0627\u0644\u062a\u062c\u0631\u0628\u0629 \u064a\u0642\u062a\u0636\u064a \u0623\u0646 \u062a\u0643\u0648\u0646 \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u0627\u062e\u062a\u0628\u0627\u0631 \u0627\u0644\u062a\u064a \u062a\u0645 \u0627\u0644\u062d\u0635\u0648\u0644 \u0639\u0644\u064a\u0647\u0627 \u062a\u0645\u062b\u0644 \u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u062c\u0632\u0621 \u0627\u0644\u0645\u0645\u062a\u0635 \u0645\u0646 \u0635\u0628\u063a\u0627\u062a \u0627\u0644\u0634\u0639\u0631 \u0639\u0644\u0649 \u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u062e\u0637 \u0627\u0644\u062e\u0644\u0648\u064a HEK293T. \u0648\u0644\u0623\u0646 \u0645\u0642\u0627\u064a\u0633\u0629 \u0627\u0644\u0646\u0648\u064a\u0629 \u0627\u0644\u0635\u063a\u064a\u0631\u0629 \u062a\u0639\u0637\u064a \u0641\u0643\u0631\u0629 \u0639\u0646 \u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u0645\u0627\u062f\u0629 \u0627\u0644\u0645\u062f\u0631\u0648\u0633\u0629 \u0639\u0644\u0649 \u0627\u0644\u0645\u0627\u062f\u0629 \u0627\u0644\u0648\u0631\u0627\u062b\u064a\u0629 \u0644\u0644\u062e\u0644\u0627\u064a\u0627 \u0643\u0627\u0646\u062a \u0646\u062a\u0627\u0626\u062c\u0647\u0627 \u0645\u0639\u0628\u0631\u0629 \u0639\u0646 \u0627\u0644\u0633\u0645\u064a\u0629 \u0627\u0644\u062c\u064a\u0646\u064a\u0629 \u0627\u0644\u0645\u062d\u062a\u0645\u0644\u0629 \u0639\u0644\u0649 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0628\u0634\u0631\u064a\u0629. <\/span>\u0623\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0623\u0646 \u0627\u0644\u0646\u0633\u0628\u0629 \u0627\u0644\u0645\u0626\u0648\u064a\u0629 \u0644\u0639\u062f\u062f \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u062d\u0627\u0648\u064a\u0629 \u0639\u0644\u0649 \u0627\u0644\u0646\u0648\u064a\u0629 \u0627\u0644\u0635\u063a\u064a\u0631\u0629 \u062a\u0631\u0627\u0648\u062d\u062a \u0628\u064a\u0646 1.2-3% \u0648\u0630\u0644\u0643 \u0628\u062d\u0633\u0628 \u0646\u0648\u0639 \u0627\u0644\u0635\u0628\u063a\u0629 \u0648\u0628\u062d\u0633\u0628 \u0627\u0644\u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0645\u0633\u062a\u0639\u0645\u0644 \u0639\u0644\u0645\u0627\u064b \u0623\u0646 \u0647\u0630\u0647 \u0627\u0644\u0646\u0633\u0628\u0629 \u0628\u0644\u063a\u062a 1.3% \u0641\u064a \u0627\u0644\u0634\u0627\u0647\u062f \u0627\u0644\u0633\u0644\u0628\u064a \u06488.3% \u0641\u064a \u0627\u0644\u0634\u0627\u0647\u062f \u0627\u0644\u0625\u064a\u062c\u0627\u0628\u064a. <\/span>\u0628\u064a\u0646\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0623\u0646 \u0627\u0644\u0635\u0628\u063a\u0629 \u0631\u0642\u0645 2 \u0642\u062f \u0623\u0628\u062f\u062a \u0623\u0643\u0628\u0631 \u0633\u0645\u064a\u0629 \u062c\u064a\u0646\u064a\u0629 \u0628\u064a\u0646 \u0627\u0644\u0635\u0628\u063a\u0627\u062a \u0627\u0644\u0623\u0631\u0628\u0639 \u0627\u0644\u0645\u0633\u062a\u0639\u0645\u0644\u0629\u060c \u0625\u0630 \u0643\u0627\u0646\u062a \u0646\u0633\u0628\u0629 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u062d\u0627\u0648\u064a\u0629 \u0639\u0644\u0649 \u0627\u0644\u0646\u0648\u064a\u0627\u062a \u0627\u0644\u0635\u063a\u064a\u0631\u0629 \u0639\u0646\u062f \u0623\u062f\u0646\u0649 \u062a\u0631\u0643\u064a\u0632 \u0645\u0633\u062a\u0639\u0645\u0644 (2.5\u00b5l\/100\u00b5l) \u0623\u0643\u0628\u0631 \u0645\u0646 \u0646\u0633\u0628\u062a\u0647\u0627 \u0641\u064a \u0627\u0644\u0634\u0627\u0647\u062f \u0628\u0641\u0627\u0631\u0642 \u0645\u0639\u0646\u0648\u064a \u062d\u064a\u062b \u0643\u0627\u0646\u062a \u0642\u064a\u0645\u0629 P value= 80.001. \u0641\u064a\u0645\u0627 \u0638\u0647\u0631 \u0627\u0644\u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u062c\u0648\u0647\u0631\u064a \u0627\u0644\u0633\u0645\u064a \u0644\u0644\u0635\u0628\u063a\u0629 \u0631\u0642\u0645 1 \u0639\u0646\u062f \u0627\u0644\u062a\u0631\u0643\u064a\u0632 10\u00b5l\/100\u00b5l \u062d\u064a\u062b \u0643\u0627\u0646\u062a \u0642\u064a\u0645\u0629 P value= 20.001\u060c \u0644\u064a\u062a\u0646\u0627\u0642\u0635 \u0639\u062f\u062f \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u062d\u0627\u0648\u064a\u0629 \u0639\u0644\u0649 \u0627\u0644\u0646\u0648\u064a\u0629 \u0627\u0644\u0635\u063a\u064a\u0631\u0629 \u0628\u0639\u062f \u0630\u0644\u0643 \u0641\u064a \u0627\u0644\u062a\u0631\u0627\u0643\u064a\u0632 \u0627\u0644\u0623\u0639\u0644\u0649 \u0645\u0646 \u0643\u0644\u062a\u0627 \u0627\u0644\u0635\u0628\u063a\u062a\u064a\u0646. \u0641\u064a\u0645\u0627 \u0644\u0645 \u062a\u0643\u0646 \u0627\u0644\u0632\u064a\u0627\u062f\u0629 \u0641\u064a \u0639\u062f\u062f \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u062d\u0627\u0648\u064a\u0629 \u0639\u0644\u0649 \u0646\u0648\u064a\u0629 \u0635\u063a\u064a\u0631\u0629 \u0639\u0646\u062f \u062a\u0637\u0628\u064a\u0642 \u0627\u0644\u0635\u0628\u063a\u062a\u064a\u0646 \u0631\u0642\u0645 3 \u06484 \u0630\u0627\u062a \u0641\u0627\u0631\u0642 \u062c\u0648\u0647\u0631\u064a \u0628\u0627\u0644\u0645\u0642\u0627\u0631\u0646\u0629 \u0645\u0639 \u0627\u0644\u0634\u0627\u0647\u062f \u062d\u064a\u062b \u0643\u0627\u0646\u062a \u0642\u064a\u0645\u0629 P value \u0645\u0633\u0627\u0648\u064a\u0629 0.76 \u06480.19 \u0639\u0644\u0649 \u0627\u0644\u062a\u0631\u062a\u064a\u0628\u060c \u0643\u0645\u0627 \u064a\u0638\u0647\u0631 \u0641\u064a \u0627\u0644\u0634\u0643\u0644 (2). <\/span>\u062a\u0645\u062b\u0644 \u0627\u0644\u0645\u062e\u0637\u0637\u0627\u062a \u0627\u0644\u0646\u0633\u0628 \u0627\u0644\u0645\u0626\u0648\u064a\u0629 \u0644\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u062d\u0627\u0648\u064a\u0629 \u0639\u0644\u0649 \u0627\u0644\u0646\u0648\u064a\u0627\u062a \u0627\u0644\u0635\u063a\u064a\u0631\u0629 \u0628\u0639\u062f \u0627\u0644\u0645\u0639\u0627\u0644\u062c\u0629 \u0628\u0623\u0631\u0628\u0639 \u062a\u0631\u0627\u0643\u064a\u0632 2.5\u060c 10\u060c 25\u060c 50 \u00b5l \u0644\u0643\u0644 100\u00b5l \u0645\u0646 \u0648\u0633\u0637 \u0627\u0644\u0632\u0631\u0639 \u0648\u0630\u0644\u0643 \u0644\u0643\u0644 \u0635\u0628\u063a\u0629 \u0645\u0646 \u0627\u0644\u0635\u0628\u063a\u0627\u062a \u0627\u0644\u0623\u0631\u0628\u0639 \u0639\u0644\u0649 \u062d\u062f\u0629 \u0627\u0644\u0623\u0639\u0645\u062f\u0629 \u0645\u0646 2 \u0625\u0644\u0649 5 \u0628\u0627\u0644\u0625\u0636\u0627\u0641\u0629 \u0625\u0644\u0649 \u0627\u0644\u0634\u0627\u0647\u062f \u0627\u0644\u0633\u0644\u0628\u064a (\u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u063a\u064a\u0631 \u0627\u0644\u0645\u0639\u0627\u0644\u062c\u0629) \u0627\u0644\u0639\u0645\u0648\u062f \u0627\u0644\u0623\u0648\u0644 \u0648\u0627\u0644\u0625\u064a\u062c\u0627\u0628\u064a \u0627\u0644\u0639\u0645\u0648\u062f \u0627\u0644\u0623\u062e\u064a\u0631 (\u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0645\u0639\u0627\u0644\u062c\u0629 \u0628\u0627\u0644\u0643\u0648\u0644\u0634\u0633\u064a\u0646). \u0639\u064f\u0631\u0636\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c 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\u0627\u0644\u0627\u0633\u062a\u0646\u062a\u0627\u062c <\/strong>Conclusions<\/strong> \u00a0<\/strong><\/span><\/p>\n

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\u0627\u0644\u0645\u0642\u062f\u0645\u0629 Introduction: \u062a\u0639\u062f \u0627\u0644\u0623\u0645\u064a\u0646\u0627\u062a \u0627\u0644\u0639\u0637\u0631\u064a\u0629 \u0648\u0627\u062d\u062f\u0629 \u0645\u0646 \u0623\u0647\u0645 \u0627\u0644\u0645\u0631\u0643\u0628\u0627\u062a \u0627\u0644\u0643\u064a\u0645\u064a\u0627\u0626\u064a\u0629 \u0627\u0644\u062a\u064a \u062a\u062f\u062e\u0644 \u0641\u064a \u0627\u0644\u0639\u062f\u064a\u062f \u0645\u0646 \u0627\u0644\u0635\u0646\u0627\u0639\u0627\u062a \u0648\u0645\u0646\u0647\u0627 \u0635\u0628\u063a\u0627\u062a \u0627\u0644\u0634\u0639\u0631 \u0639\u0644\u0649 \u0627\u0644\u0631\u063a\u0645 \u0645\u0646…
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