{"id":2129,"date":"2026-03-15T13:54:37","date_gmt":"2026-03-15T13:54:37","guid":{"rendered":"https:\/\/pharmjournal.asscph.com\/?p=2129"},"modified":"2026-03-15T14:09:35","modified_gmt":"2026-03-15T14:09:35","slug":"%d8%aa%d9%82%d9%8a%d9%8a%d9%85-%d8%a7%d9%84%d9%81%d8%b9%d8%a7%d9%84%d9%8a%d8%a9-%d8%a7%d9%84%d9%85%d8%b6%d8%a7%d8%af%d8%a9-%d9%84%d9%84%d9%85%d9%8a%d9%83%d8%b1%d9%88%d8%a8%d8%a7%d8%aa-%d9%84%d8%a8","status":"publish","type":"post","link":"https:\/\/pharmjournal.asscph.com\/?p=2129","title":{"rendered":"\u062a\u0642\u064a\u064a\u0645 \u0627\u0644\u0641\u0639\u0627\u0644\u064a\u0629 \u0627\u0644\u0645\u0636\u0627\u062f\u0629 \u0644\u0644\u0645\u064a\u0643\u0631\u0648\u0628\u0627\u062a \u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0625\u064a\u0648\u0645\u064a\u0646\u064a\u062a\u064a\u0646 \u0627\u0644\u0645\u0624\u0634\u0628 \u0648\u0627\u0644\u0645\u0634\u062a\u0642 \u0645\u0646 \u0645\u0643\u0648\u0646\u0627\u062a \u0632\u064f\u0639\u0627\u0641 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\u0648\u0628\u0634\u0643\u0644 \u062e\u0627\u0635 \u0641\u064a \u0646\u0638\u0627\u0645 <em>E.coli<\/em>\u060c \u0641\u0639\u0627\u0644\u064a\u062a\u0647\u0627 \u0643\u0623\u062d\u062f \u0631\u0643\u0627\u0626\u0632 \u00a0\u0627\u0644\u062a\u0642\u0627\u0646\u0629 \u0627\u0644\u062d\u064a\u0648\u064a\u0629 \u0627\u0644\u062d\u062f\u064a\u062b\u0629 \u0641\u064a \u0625\u0646\u062a\u0627\u062c \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0627\u0644\u0639\u0644\u0627\u062c\u064a\u0629 [7]. \u0648\u064a\u0643\u062a\u0633\u0628 \u0647\u0630\u0627 \u0627\u0644\u0646\u0647\u062c \u0623\u0647\u0645\u064a\u0629 \u0645\u062a\u0632\u0627\u064a\u062f\u0629\u060c \u0644\u0627 \u0633\u064a\u0645\u0627 \u0641\u064a \u0638\u0644 \u0635\u0639\u0648\u0628\u0629 \u0627\u0633\u062a\u062e\u0644\u0627\u0635 \u0627\u0644\u0628\u0628\u062a\u064a\u062f\u0627\u062a \u0630\u0627\u062a \u0627\u0644\u0648\u0632\u0646 \u0627\u0644\u062c\u0632\u064a\u0626\u064a \u0627\u0644\u0645\u0646\u062e\u0641\u0636 \u0645\u0646 \u0645\u0635\u0627\u062f\u0631\u0647\u0627 \u0627\u0644\u0637\u0628\u064a\u0639\u064a\u0629 \u0628\u0643\u0645\u064a\u0627\u062a \u0643\u0627\u0641\u064a\u0629 \u0648\u0643\u0644\u0641\u0629 \u0627\u0642\u062a\u0635\u0627\u062f\u064a\u0629 [8].\u0648\u0642\u062f \u0623\u064f\u0646\u062a\u062c\u062a \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0647\u0630\u0627 \u0627\u0644\u0646\u0638\u0627\u0645 \u0628\u0628\u062a\u064a\u062f\u0627\u062a \u062f\u0648\u0627\u0626\u064a\u0629 \u0639\u0627\u0644\u064a\u0629 \u0627\u0644\u0641\u0639\u0627\u0644\u064a\u0629 \u0645\u0634\u062a\u0642\u0629 \u0645\u0646 \u0633\u0645\u0648\u0645 \u062d\u064a\u0648\u0627\u0646\u064a\u0629\u060c \u0645\u062b\u0644 Eptifibatide (Integrilin\u00ae) \u0648Tirofiban (Aggrastat\u00ae)\u060c \u0648\u0643\u0644\u0627\u0647\u0645\u0627 \u064a\u064f\u0633\u062a\u062e\u062f\u0645 \u0633\u0631\u064a\u0631\u064a\u064b\u0627 \u0643\u0645\u062b\u0628\u0637 \u0644\u062a\u0643\u062f\u0633 \u0627\u0644\u0635\u0641\u064a\u062d\u0627\u062a \u0627\u0644\u062f\u0645\u0648\u064a\u0629\u060c \u0648\u0642\u062f \u062a\u0645 \u062a\u0637\u0648\u064a\u0631\u0647\u0645\u0627 \u0645\u0646 \u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0645\u0648\u062c\u0648\u062f\u0629 \u0641\u064a \u0633\u0645 \u0627\u0644\u0623\u0641\u0627\u0639\u064a [9]. \u0648\u0628\u0627\u0644\u0627\u0633\u062a\u0641\u0627\u062f\u0629 \u0645\u0646 \u0627\u0644\u0646\u062c\u0627\u062d\u0627\u062a \u0627\u0644\u0645\u0633\u062c\u0644\u0629 \u0641\u064a \u062a\u062d\u0648\u064a\u0644 \u0645\u0643\u0648\u0646\u0627\u062a \u0627\u0644\u0633\u0645\u0648\u0645 \u0627\u0644\u062d\u064a\u0648\u0627\u0646\u064a\u0629 \u0625\u0644\u0649 \u0623\u062f\u0648\u064a\u0629 \u0645\u0628\u062a\u0643\u0631\u0629 \u0639\u0628\u0631 \u0623\u0646\u0638\u0645\u0629 \u0627\u0644\u062a\u0623\u0634\u064a\u0628\u060c \u062a\u0632\u0627\u064a\u062f \u0627\u0644\u0627\u0647\u062a\u0645\u0627\u0645 \u0628\u062f\u0631\u0627\u0633\u0629 \u0645\u0631\u0643\u0628\u0627\u062a \u062c\u062f\u064a\u062f\u0629 \u0630\u0627\u062a \u0646\u0634\u0627\u0637 \u0645\u064a\u0643\u0631\u0648\u0628\u064a \u0645\u062d\u062a\u0645\u0644 \u064a\u0645\u0643\u0646 \u0625\u0646\u062a\u0627\u062c\u0647\u0627 \u0645\u062e\u0628\u0631\u064a\u0627\u064b \u0628\u0643\u0641\u0627\u0621\u0629 \u0648\u0628\u062a\u0643\u0644\u0641\u0629 \u0645\u0646\u062e\u0641\u0636\u0629.<\/p>\n<p>\u0648\u0641\u064a \u0627\u0644\u0633\u064a\u0627\u0642 \u0630\u0627\u062a\u0647\u060c \u064a\u064f\u0639\u062f \u0627\u0644\u0625\u064a\u0648\u0645\u064a\u0646\u064a\u062a\u064a\u0646 (Eumenitin) \u0628\u0628\u062a\u064a\u062f\u0627\u064b \u0643\u0627\u062a\u064a\u0648\u0646\u064a\u0627\u064b \u062e\u0637\u064a\u0627\u064b \u0645\u0643\u0648\u0646\u0627\u064b \u0645\u0646 15 \u062d\u0645\u0636\u064b\u0627 \u0623\u0645\u064a\u0646\u064a\u064b\u0627 (ILGTILGLLKGLGVF) \u0648\u0647\u0648 \u0623\u062d\u062f \u0627\u0644\u0645\u0643\u0648\u0646\u0627\u062a \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0629 \u0644\u0632\u064f\u0639\u0627\u0641 \u062f\u0628\u0648\u0631 <em>Eumenes rubronotatus<\/em>. \u00a0\u0648\u064a\u062a\u062e\u0630 \u0647\u0630\u0627 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0628\u0646\u064a\u0629 \u062d\u0644\u0632\u0648\u0646\u064a\u0629 \u0648\u064a\u0645\u062a\u0627\u0632 \u0628\u062e\u0648\u0627\u0635 \u0645\u062d\u0628\u0629 \u0644\u0644\u0645\u0627\u0621 \u0648\u0627\u0644\u062f\u0633\u0645 \u0645\u0639\u064b\u0627 (Amphiphilic properties)\u060c \u0648\u064a\u064f\u0638\u0647\u0631 \u0646\u0634\u0627\u0637\u064b\u0627 \u0636\u062f \u0645\u062c\u0645\u0648\u0639\u0629 \u0645\u0646 \u0627\u0644\u062c\u0631\u0627\u062b\u064a\u0645 \u0645\u0648\u062c\u0628\u0629 \u0648\u0633\u0627\u0644\u0628\u0629 \u0627\u0644\u063a\u0631\u0627\u0645\u060c \u0628\u0627\u0644\u0625\u0636\u0627\u0641\u0629 \u0625\u0644\u0649 \u062a\u0623\u062b\u064a\u0631 \u0645\u062a\u0648\u0633\u0637 \u0636\u062f \u0637\u0641\u064a\u0644\u064a \u0627\u0644\u0644\u0627\u0634\u0645\u0627\u0646\u064a\u0627 \u0648\u0646\u0634\u0627\u0637 \u0645\u062d\u0641\u0632 \u0641\u064a \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0628\u062f\u064a\u0646\u0629 (<em>Mast cell degradation<\/em>) \u0648\u0642\u062f \u0628\u064a\u0651\u0646\u062a \u0627\u0644\u062f\u0631\u0627\u0633\u0627\u062a \u0623\u0646 \u0627\u0644\u0625\u064a\u0648\u0645\u064a\u0646\u064a\u062a\u064a\u0646 \u064a\u062a\u0645\u062a\u0639 \u0628\u0647\u0627\u0645\u0634 \u0623\u0645\u0627\u0646 \u062c\u064a\u062f\u060c \u0625\u0630 \u0644\u0645 \u064a\u064f\u062d\u062f\u062b \u0627\u0646\u062d\u0644\u0627\u0644\u064b\u0627 \u0641\u064a \u0643\u0631\u064a\u0627\u062a \u0627\u0644\u062f\u0645 \u0627\u0644\u062d\u0645\u0631\u0627\u0621 \u0636\u0645\u0646 \u0627\u0644\u062a\u0631\u0643\u064a\u0632\u0627\u062a \u0627\u0644\u0641\u0639\u0627\u0644\u0629. \u0648\u062a\u0639\u062a\u0645\u062f \u0622\u0644\u064a\u0629 \u0639\u0645\u0644 \u0627\u0644\u0625\u064a\u0648\u0645\u064a\u0646\u064a\u062a\u064a\u0646 \u0639\u0644\u0649 \u0646\u0645\u0648\u0630\u062c &#8220;\u0627\u0644\u062b\u0642\u0628 \u0627\u0644\u062d\u0644\u0642\u064a(<em>Toroidal pore<\/em>)\u060c \u062d\u064a\u062b \u064a\u062a\u0641\u0627\u0639\u0644 \u0645\u0639 \u0627\u0644\u0644\u064a\u0628\u064a\u062f\u0627\u062a \u0641\u064a \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u062e\u0644\u0648\u064a \u0644\u062a\u0643\u0648\u064a\u0646 \u0642\u0646\u0627\u0629 \u0645\u0633\u062a\u0645\u0631\u0629\u060c \u0645\u0627 \u064a\u0624\u062f\u064a \u0625\u0644\u0649 \u0627\u062e\u062a\u0644\u0627\u0644 \u0646\u0641\u0627\u0630\u064a\u0629 \u0627\u0644\u063a\u0634\u0627\u0621 \u062f\u0648\u0646 \u0627\u0633\u062a\u0647\u062f\u0627\u0641 \u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0645\u062d\u062f\u062f\u0629\u060c \u0645\u0645\u0627 \u064a\u064f\u0635\u0639\u0651\u0628 \u0639\u0644\u0649 \u0627\u0644\u062c\u0631\u0627\u062b\u064a\u0645 \u062a\u0637\u0648\u064a\u0631 \u0645\u0642\u0627\u0648\u0645\u0629 \u0648\u0631\u0627\u062b\u064a\u0629 \u0641\u0639\u0627\u0644\u0629 [10].<\/p>\n<p>\u0641\u064a \u0647\u0630\u0647 \u0627\u0644\u062f\u0631\u0627\u0633\u0629\u060c \u062a\u0645 \u0625\u0646\u062a\u0627\u062c \u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0625\u064a\u0648\u0645\u064a\u0646\u064a\u062a\u064a\u0646 (Eumenitin) \u0628\u0637\u0631\u064a\u0642\u0629 \u0627\u0644\u062a\u0623\u0634\u064a\u0628 \u0641\u064a <em>E.coli<\/em>\u060c \u0644\u062a\u0642\u064a\u064a\u0645 \u0641\u0627\u0639\u0644\u064a\u062a\u0647 \u0627\u0644\u0645\u0636\u0627\u062f\u0629 \u0644\u0644\u062c\u0631\u0627\u062b\u064a\u0645 \u0628\u0647\u062f\u0641 \u0627\u0644\u062a\u062d\u0642\u0642 \u0645\u0646 \u0645\u062f\u0649 \u0643\u0641\u0627\u0621\u0629 \u0625\u0646\u062a\u0627\u062c\u0647 \u0648\u0627\u062d\u062a\u0641\u0627\u0638\u0647 \u0628\u0641\u0639\u0627\u0644\u064a\u062a\u0647 \u0627\u0644\u062d\u064a\u0648\u064a\u0629\u060c \u0645\u0645\u0627 \u0643\u0645\u0648\u0646\u0647 \u0627\u0644\u0639\u0644\u0627\u062c\u064a \u0627\u0644\u0645\u0636\u0627\u062f \u0644\u0644\u062c\u0631\u0627\u062b\u064a\u0645 \u0641\u064a \u0627\u0644\u0645\u0633\u062a\u0642\u0628\u0644.<\/p>\n<p><strong>\u0627\u0644\u0645\u0648\u0627\u062f \u0648\u0627\u0644\u0637\u0631\u0627\u0626\u0642 <\/strong><strong>Materials and Methods<\/strong><\/p>\n<p><strong>\u0625\u0646\u062a\u0627\u062c \u0648\u062a\u0646\u0642\u064a\u0629 \u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0625\u064a\u0648\u0645\u064a\u0646\u064a\u062a\u064a\u0646 \u0627\u0644\u0645\u0624\u0634\u0628 <\/strong><\/p>\n<p><strong>\u0625\u0646\u062a\u0627\u062c \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0645\u0624\u0634\u0628<\/strong><\/p>\n<p>\u0627\u0633\u062a\u062e\u062f\u0645\u062a \u0627\u0644\u0633\u0644\u0627\u0644\u0629 \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0629 <em>E. coli<\/em> Top10 \u00a0\u0641\u064a \u062e\u0637\u0648\u0627\u062a \u062a\u0646\u0633\u064a\u0644 \u0627\u0644\u062c\u064a\u0646\u060c \u0628\u064a\u0646\u0645\u0627 \u0627\u0633\u062a\u064f\u062e\u062f\u0645\u062a \u0627\u0644\u0633\u0644\u0627\u0644\u0629 <em>E. coli<\/em> BL21 (Gold) \u0641\u064a \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u064a. \u0648\u0644\u063a\u0631\u0636 \u062a\u0646\u0633\u064a\u0644 \u062c\u064a\u0646 \u0627\u0644\u062f\u064a\u0643\u0648\u0631\u0627\u0644\u064a\u0646 \u0648\u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0639\u0646\u0647\u060c \u062a\u0645 \u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0628\u0644\u0627\u0633\u0645\u064a\u062f\u0627\u062a \u0645\u064f\u0635\u0645\u0645\u0629 \u0645\u0633\u0628\u0642\u064b\u0627 \u0648\u0647\u064a \u0628\u0644\u0627\u0633\u0645\u064a\u062f \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u064a pRSET (Invitrogen) \u0648\u0627\u0644\u0628\u0644\u0627\u0633\u0645\u064a\u062f \u0627\u0644\u0645\u0634\u062a\u0642 \u0639\u0646\u0647 pRSET-sfGFP\u060c \u062d\u064a\u062b \u062c\u0631\u0649 \u0625\u062f\u062e\u0627\u0644 \u0627\u0644\u062c\u064a\u0646 \u0627\u0644\u0647\u062f\u0641 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0628\u0631\u0646\u0627\u0645\u062c Geneious [11]. \u0648\u0627\u062e\u062a\u064a\u0631 \u0627\u0644\u0628\u0644\u0627\u0633\u0645\u064a\u062f \u0627\u0644\u062a\u062c\u0627\u0631\u064a pRSET (Invitrogen) \u0644\u0627\u062d\u062a\u0648\u0627\u0626\u0647 \u0639\u0644\u0649 \u0645\u062d\u0641\u0632 T7 \u0639\u0627\u0644\u064a \u0627\u0644\u0643\u0641\u0627\u0621\u0629 \u0648\u0648\u0633\u0645\u0629 \u0633\u062f\u0627\u0633\u064a\u0629 \u0627\u0644\u0647\u0633\u062a\u064a\u062f\u064a\u0646 (His\u2086-tag) \u0639\u0646\u062f \u0627\u0644\u0646\u0647\u0627\u064a\u0629 \u0627\u0644\u0623\u0645\u064a\u0646\u064a\u0629\u060c \u0645\u0645\u0627 \u064a\u0633\u0647\u0644 \u0639\u0645\u0644\u064a\u0629 \u062a\u0646\u0642\u064a\u0629 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0644\u0627\u062d\u0642\u064b\u0627. \u0643\u0645\u0627 \u0627\u0633\u062a\u064f\u062e\u062f\u0645 \u0628\u0644\u0627\u0633\u0645\u064a\u062f \u0645\u0634\u062a\u0642 \u0647\u0648 pRSET-sfGFP \u0645\u062a\u0648\u0641\u0631 \u0641\u064a \u0647\u064a\u0626\u0629 \u0627\u0644\u0637\u0627\u0642\u0629 \u0627\u0644\u0630\u0631\u064a\u0629\u060c \u064a\u062a\u0636\u0645\u0646 \u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0641\u0644\u0648\u0631\u064a \u0627\u0644\u0623\u062e\u0636\u0631 \u0627\u0644\u0641\u0627\u0626\u0642 (sfGFP)\u060c \u0643\u0648\u0627\u0633\u0645 \u0644\u062a\u062a\u0628\u0639 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u064a \u0648\u062a\u062d\u0633\u064a\u0646 \u0637\u064a\u0651 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u062f\u0627\u062e\u0644 \u0627\u0644\u062e\u0644\u064a\u0629.\u0646\u064f\u0645\u064a\u062a \u0627\u0644\u0633\u0644\u0627\u0644\u0627\u062a \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0629 \u0641\u064a \u0648\u0633\u0637 Luria-Bertani (LB)\u060c \u0628\u064a\u0646\u0645\u0627 \u0627\u0633\u062a\u062e\u062f\u0645 \u0648\u0633\u0637 \u00a0\u00a0Yeast Tryptone (2YT) \u0623\u062b\u0646\u0627\u0621 \u062a\u0647\u064a\u0626\u0629 \u0627\u0644\u062e\u0644\u0627\u064a\u0627\u060c \u0648\u0627\u0633\u062a\u062e\u062f\u0645 \u0623\u062b\u0646\u0627\u0621 \u062a\u062d\u0648\u064a\u0631 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0648\u0633\u0637 (SOB) Super Optimal Broth \u00a0\u0647\u0648 \u0648\u0633\u0637 \u063a\u0630\u0627\u0626\u064a \u064a\u064f\u0633\u062a\u062e\u062f\u0645 \u0628\u0634\u0643\u0644 \u0623\u0633\u0627\u0633\u064a \u0644\u062a\u062d\u0636\u064a\u0631 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0629 \u0627\u0644\u0643\u0647\u0631\u0648\u0636\u0648\u0626\u064a\u0629\u060c \u0644\u0627\u062d\u062a\u0648\u0627\u0626\u0647 \u0639\u0644\u0649 \u0645\u0643\u0648\u0646\u0627\u062a \u062a\u062f\u0639\u0645 \u0646\u0645\u0648 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0648\u062a\u062d\u0633\u064a\u0646 \u0643\u0641\u0627\u0621\u062a\u0647\u0627 \u0641\u064a \u0627\u0644\u062a\u062d\u0648\u064a\u0631. \u0623\u0636\u064a\u0641 \u0627\u0644\u0645\u0636\u0627\u062f \u0627\u0644\u062d\u064a\u0648\u064aCarbenicillin\u00a0 \u00a0\u0628\u062a\u0631\u0643\u064a\u0632 \u0645\u0646\u0627\u0633\u0628 \u0644\u0636\u0645\u0627\u0646 \u0627\u0646\u062a\u0642\u0627\u0621 \u0627\u0644\u0633\u0644\u0627\u0644\u0627\u062a \u0627\u0644\u0645\u062d\u0648\u0651\u0631\u0629 \u0627\u0644\u0645\u0642\u0627\u0648\u0645\u0629 \u0644\u0647\u060c \u0648\u062a\u0645 \u062a\u062d\u0636\u064a\u0646 \u0627\u0644\u0632\u0631\u0639\u0627\u062a \u0639\u0646\u062f 37\u00b0\u0645 \u0637\u0648\u0627\u0644 \u0627\u0644\u0644\u064a\u0644.<\/p>\n<p>\u0635\u064f\u0645\u0645 \u062c\u064a\u0646 \u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0625\u064a\u0648\u0645\u064a\u0646\u064a\u062a\u064a\u0646 (Eumenitin) \u0628\u0646\u0627\u0621\u064b \u0639\u0644\u0649 \u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u0623\u062d\u0645\u0627\u0636 \u0627\u0644\u0623\u0645\u064a\u0646\u064a\u0629 \u0627\u0644\u0645\u0633\u062a\u062e\u0644\u0635 \u0645\u0646 \u0627\u0644\u0628\u0646\u064a\u0629 \u0627\u0644\u0641\u0631\u0627\u063a\u064a\u0629 \u0644\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0645\u0631\u062c\u0639\u064a \u0627\u0644\u0645\u0633\u062c\u0644 \u0641\u064a \u0642\u0627\u0639\u062f\u0629 \u0628\u064a\u0627\u0646\u0627\u062a PDB (ID: 2N9A, Chain A)\u060c \u0648\u0642\u062f \u062a\u0645 \u062a\u062d\u0633\u064a\u0646 \u0627\u0644\u0643\u0648\u062f\u0648\u0646\u0627\u062a \u0644\u062a\u062a\u0644\u0627\u0621\u0645 \u0645\u0639 \u0646\u0638\u0627\u0645 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0641\u064a E. coli \u0628\u0647\u062f\u0641 \u0631\u0641\u0639 \u0643\u0641\u0627\u0621\u0629 \u0627\u0644\u062a\u0631\u062c\u0645\u0629 [12]. \u062c\u0631\u0649 \u062a\u062c\u0645\u064a\u0639 \u0627\u0644\u062a\u0633\u0644\u0633\u0644 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062a\u0642\u0646\u064a\u0629 One-pot assembly \u00a0\u0645\u0646\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0oligonucleotides \u0645\u0635\u0645\u0645\u0629 \u062e\u0635\u064a\u0635\u064b\u0627 \u0645\u0646 \u0645\u0633\u0628\u0631\u064a\u0646 \u0645\u062a\u062a\u0627\u0645\u064a\u0646 \u0627\u0644\u062c\u062f\u0648\u0644 (1)\u060c \u0645\u0639 \u0625\u062f\u0631\u0627\u062c \u0645\u0648\u0627\u0642\u0639 \u0625\u0646\u0632\u064a\u0645\u0627\u062a \u0627\u0644\u062a\u0642\u064a\u064a\u062fApaI \u00a0\u0648HindIII\u060c \u0628\u0627\u0644\u0625\u0636\u0627\u0641\u0629 \u0625\u0644\u0649 \u0645\u0648\u0642\u0639 \u0625\u0646\u0632\u064a\u0645 TEV protease \u00a0\u0648\u0648\u0633\u0645\u0629- Tag \u00a0\u00a0.His6<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-2131 aligncenter\" src=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/1-300x44.png\" alt=\"\" width=\"607\" height=\"89\" srcset=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/1-300x44.png 300w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/1-768x112.png 768w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/1.png 907w\" sizes=\"auto, (max-width: 607px) 100vw, 607px\" \/><\/p>\n<p>\u062a\u0645 \u062a\u0636\u062e\u064a\u0645 \u0627\u0644\u0634\u062f\u0641\u0629 \u0627\u0644\u062c\u064a\u0646\u064a\u0629 \u0639\u0628\u0631 \u0645\u0631\u062d\u0644\u062a\u064a\u0646 \u0645\u0646 \u062a\u0641\u0627\u0639\u0644 \u0627\u0644\u0628\u0644\u0645\u0631\u0629 \u0627\u0644\u0645\u062a\u0633\u0644\u0633\u0644 (PCR)\u060c \u062d\u064a\u062b \u062d\u064f\u0636\u0651\u0650\u0631 \u062d\u062c\u0645 \u0646\u0647\u0627\u0626\u064a \u0642\u062f\u0631\u0647 \u00b5l 25 \u064a\u062d\u062a\u0648\u064a \u0639\u0644\u0649 \u00b5M 0.5 \u0645\u0646 \u0643\u0644 \u0645\u0631\u0626\u0633\u0629 \u0636\u0645\u0646 \u0627\u0644\u0634\u0631\u0648\u0637 \u0627\u0644\u0622\u062a\u064a\u0629: 4 \u062f\u0642\u0627\u0626\u0642 \u0641\u064a 94\u00b0\u0645\u060c \u062b\u0645 35 \u062f\u0648\u0631\u0629 (30 \u062b\u0627\u0646\u064a\u0629 \u0641\u064a 94\u00b0\u0645\u060c 30 \u062b\u0627\u0646\u064a\u0629 \u0641\u064a 60\u00b0\u0645\u060c \u064830 \u062b\u0627\u0646\u064a\u0629 \u0641\u064a 72\u00b0\u0645)\u060c \u0645\u0639 7 \u062f\u0642\u0627\u0626\u0642 \u0641\u064a 72\u00b0\u0645. \u0634\u0645\u0644\u062a \u062a\u0641\u0627\u0639\u0644\u064b\u0627 \u0623\u0648\u0644\u064a\u064b\u0627 \u0644\u0625\u0646\u062a\u0627\u062c \u0642\u0627\u0644\u0628 \u0623\u0648\u0644\u064a \u064a\u0645\u062b\u0644 \u0634\u062f\u0641\u0629 \u0627\u0644\u0625\u064a\u0648\u0645\u064a\u0646\u064a\u062a\u064a\u0646\u060c \u0623\u0639\u0642\u0628\u0647 \u062a\u0641\u0627\u0639\u0644 \u0628\u0644\u0645\u0631\u0629 \u0645\u062a\u0639\u0627\u0642\u0628 (Nested PCR) \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0628\u0627\u062f\u0626\u064a\u0646 \u0645\u0633\u062a\u0645\u062f\u064a\u0646 \u0645\u0646 \u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u0628\u0644\u0627\u0633\u0645\u064a\u062f \u0627\u0644\u062d\u0627\u0648\u064a \u0639\u0644\u0649 \u0627\u0644\u062c\u064a\u0646. \u0648\u0644\u062a\u062d\u0642\u064a\u0642 \u062f\u0642\u0629 \u0639\u0627\u0644\u064a\u0629\u060c \u0627\u0633\u062a\u064f\u062e\u062f\u0645 \u0625\u0646\u0632\u064a\u0645 \u0628\u0644\u0645\u0631\u0629 \u0639\u0627\u0644\u064a \u0627\u0644\u062f\u0642\u0629 (High-fidelity DNA polymerase) \u0628\u062a\u0631\u0643\u064a\u0632 U 1.25 \u0644\u062a\u0642\u0644\u064a\u0644 \u0646\u0633\u0628\u0629 \u0627\u0644\u0623\u062e\u0637\u0627\u0621 \u0627\u0644\u0646\u0627\u062a\u062c\u0629 \u0639\u0646 \u0627\u0644\u0627\u0633\u062a\u0646\u0633\u0627\u062e \u0627\u0644\u0634\u0643\u0644 (1).<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-2132 aligncenter\" src=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/2-300x78.png\" alt=\"\" width=\"550\" height=\"143\" srcset=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/2-300x78.png 300w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/2-768x201.png 768w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/2.png 850w\" sizes=\"auto, (max-width: 550px) 100vw, 550px\" \/><\/p>\n<p>\u062a\u0645 \u0625\u062c\u0631\u0627\u0621 \u0627\u0644\u062a\u062d\u0648\u064a\u0631 \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a Bacterial transformation \u0628\u0627\u0644\u062a\u062e\u0631\u064a\u0645 \u0627\u0644\u0643\u0647\u0631\u0628\u0627\u0626\u064a \u0644 \u0633\u0644\u0627\u0644\u0629 E. Coli Top 10 \u0641\u064a \u0645\u0631\u062d\u0644\u0629 \u0627\u0644\u062a\u0646\u0633\u064a\u0644. \u0627\u0639\u062a\u0645\u062f\u062a \u0647\u0630\u0647 \u0627\u0644\u0637\u0631\u064a\u0642\u0629 \u0644\u0623\u0646\u0647\u0627 \u0623\u0642\u0644 \u062a\u0639\u0642\u064a\u062f\u0627\u064b \u0648\u0623\u0643\u062b\u0631 \u0641\u0639\u0627\u0644\u064a\u0629 \u0645\u0646 \u0627\u0644\u0637\u0631\u064a\u0642\u0629 \u0627\u0644\u0643\u064a\u0645\u064a\u0627\u0626\u064a\u0629 \u0625\u0630 \u062a\u0639\u062a\u0645\u062f \u0639\u0644\u0649 \u0632\u064a\u0627\u062f\u0629 \u0646\u0641\u0627\u0630\u064a\u0629 \u0627\u0644\u063a\u0634\u0627\u0621 \u0627\u0644\u0633\u064a\u062a\u0648\u0628\u0644\u0627\u0633\u0645\u064a \u0644\u0644\u062e\u0644\u064a\u0629 \u0648\u0625\u0645\u0643\u0627\u0646\u064a\u0629 \u0625\u062d\u062f\u0627\u062b \u062b\u0642\u0648\u0628 \u0641\u064a\u0647 \u0639\u0646 \u0637\u0631\u064a\u0642 \u062a\u0637\u0628\u064a\u0642 \u062d\u0642\u0644 \u0643\u0647\u0631\u0628\u0627\u0626\u064a \u0639\u0627\u0644\u064a \u0627\u0644\u0641\u0648\u0644\u0637\u064a\u0629 \u0648\u0628\u0627\u0644\u062a\u0627\u0644\u064a \u0625\u0645\u0643\u0627\u0646\u064a\u0629\u00a0 \u0639\u0628\u0648\u0631 \u0627\u0644\u062f\u0646\u0627 \u0627\u0644\u0645\u0634\u062d\u0648\u0646 \u0633\u0644\u0628\u0627\u064b \u0644\u062f\u0627\u062e\u0644 \u0627\u0644\u062e\u0644\u064a\u0629. \u0648\u0642\u062f \u062a\u0645 \u062a\u0647\u064a\u0626\u0629 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 \u0642\u0628\u0644 \u0627\u0633\u062a\u062e\u062f\u0627\u0645\u0647\u0627 \u0641\u064a \u0639\u0645\u0644\u064a\u0629 \u0627\u0644\u062a\u062d\u0648\u064a\u0631 \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a \u0644\u0631\u0641\u0639 \u062d\u064a\u0648\u064a\u062a\u0647\u0627 \u0648\u0643\u0641\u0627\u0621\u062a\u0647\u0627\u060c \u0628\u063a\u0633\u0644\u0647\u0627 \u0644\u062a\u062e\u0644\u064a\u0635\u0647\u0627 \u0645\u0646 \u0627\u0644\u0623\u0645\u0644\u0627\u062d \u0648\u0627\u0644\u0641\u0636\u0644\u0627\u062a \u0627\u0644\u0646\u0627\u062a\u062c\u0629 \u0639\u0646 \u062a\u063a\u0630\u064a\u062a\u0647\u0627 \u0648\u062d\u0641\u0638\u0647\u0627 \u0628\u0645\u0648\u0627\u062f \u062d\u0627\u0648\u064a\u0629 \u0639\u0644\u0649 \u0645\u0635\u062f\u0631 \u0643\u0631\u0628\u0648\u0646\u064a [13].<\/p>\n<p>\u0627\u0633\u062a\u0639\u0645\u0644 \u0627\u0644\u0631\u062d\u0644\u0627\u0646 \u0627\u0644\u0643\u0647\u0631\u0628\u0627\u0626\u064a \u0639\u0644\u0649 \u0647\u0644\u0627\u0645\u0629 \u0627\u0644\u0623\u063a\u0627\u0631\u0648\u0632 \u0628\u062a\u0631\u0643\u064a\u0632 1.5% \u0644\u062a\u062d\u062f\u064a\u062f \u0623\u0637\u0648\u0627\u0644 \u0634\u062f\u0641 \u0627\u0644\u062f\u0646\u0627 DNA \u0648\u0642\u062f \u062a\u0645 \u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0648\u0627\u0633\u0645 \u0623\u0637\u0648\u0627\u0644 \u0645\u0639\u064a\u0627\u0631\u064a\u0629 (100 bp DNA ladder\u060c \u00a0Promega) \u064a\u062a\u0631\u0627\u0648\u062d \u0628\u064a\u0646 100\u20131500 \u0632\u0648\u062c \u0642\u0627\u0639\u062f\u064a \u0644\u062a\u0642\u062f\u064a\u0631 \u0623\u0637\u0648\u0627\u0644 \u0634\u062f\u0641 \u0627\u0644\u062f\u0646\u0627 \u0628\u062f\u0642\u0629. \u062a\u0645 \u062a\u0646\u0642\u064a\u0629 \u0646\u0627\u062a\u062c \u062a\u0641\u0627\u0639\u0644 PCR \u0628\u0639\u062f \u0627\u0644\u062a\u0631\u062d\u064a\u0644 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0637\u0627\u0642\u0645 Wizard SV gel and PCR clean \u2013 Up system (promega) kit\u060c \u062b\u0645 \u0623\u064f\u062f\u0631\u062c\u062a \u0627\u0644\u0634\u062f\u0641\u0629 \u0641\u064a \u0628\u0644\u0627\u0633\u0645\u064a\u062f \u0627\u0644\u062a\u0639\u0628\u064a\u0631 pRSET-sfGFP..<\/p>\n<p>&nbsp;<\/p>\n<p>\u062a\u0645\u062a \u0639\u0645\u0644\u064a\u0629 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u064a \u0648\u0641\u0642\u064b\u0627 \u0644\u062f\u0631\u0627\u0633\u0629 Sambrook \u0648\u0622\u062e\u0631\u0648\u0646 [14]\u060c \u0641\u0628\u0639\u062f \u062a\u062d\u0648\u064a\u0631 \u062e\u0644\u0627\u064a\u0627 E. coli BL21 (Gold) \u0628\u0627\u0644\u0628\u0644\u0627\u0633\u0645\u064a\u062f pRSET-sfGFP \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0635\u062f\u0645\u0629 \u0627\u0644\u062d\u0631\u0627\u0631\u064a\u0629\u060c \u0632\u064f\u0631\u0639\u062a \u0627\u0644\u0645\u0633\u062a\u0639\u0645\u0631\u0627\u062a \u0627\u0644\u0645\u062e\u062a\u0627\u0631\u0629 \u0641\u064a \u0648\u0633\u0637 (LB) \u064a\u062d\u0648\u064a \u0639\u0644\u0649 \u0627\u0644\u0645\u0636\u0627\u062f \u0627\u0644\u062d\u064a\u0648\u064a \u0643\u0627\u0631\u0628\u0646\u0633\u064a\u0644\u064a\u0646. \u0639\u0646\u062f \u0648\u0635\u0648\u0644 \u0627\u0644\u0646\u0645\u0648 \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a \u0625\u0644\u0649 \u0643\u062b\u0627\u0641\u0629 \u0636\u0648\u0626\u064a\u0629 (OD600 \u2248 0.6)\u060c \u062a\u0645 \u062a\u062d\u0641\u064a\u0632 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u064a \u0628\u0625\u0636\u0627\u0641\u0629 IPTG \u0628\u062a\u0631\u0643\u064a\u0632 0.3 \u0645\u0644\u064a\u0645\u0648\u0644\/\u0644\u062a\u0631\u060c \u0645\u0639 \u0645\u062a\u0627\u0628\u0639\u0629 \u0627\u0644\u062a\u062d\u0636\u064a\u0646 \u0639\u0646\u062f 28\u00b0\u0645 \u0644\u0645\u062f\u0629 16 \u0633\u0627\u0639\u0629.<\/p>\n<p>\u062c\u064f\u0645\u0639\u062a \u0627\u0644\u0639\u064a\u0646\u0627\u062a \u0641\u064a \u062b\u0644\u0627\u062b \u0646\u0642\u0627\u0637 \u0632\u0645\u0646\u064a\u0629 (T0\u060c T6\u060c T16) \u0644\u062a\u0642\u064a\u064a\u0645 \u0645\u0633\u062a\u0648\u064a\u0627\u062a \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u064a\u060c \u0648\u0630\u0644\u0643 \u0628\u062b\u0644\u0627\u062b \u0645\u0643\u0631\u0631\u0627\u062a \u0645\u0633\u062a\u0642\u0644\u0629 \u0644\u0636\u0645\u0627\u0646 \u0627\u0644\u062f\u0642\u0629 \u0627\u0644\u0625\u062d\u0635\u0627\u0626\u064a\u0629. \u062c\u0631\u0649 \u062a\u062d\u0644\u064a\u0644 \u0645\u0633\u062a\u0648\u064a\u0627\u062a \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0631\u062d\u0644\u0627\u0646 \u0627\u0644\u0643\u0647\u0631\u0628\u0627\u0626\u064a \u0644\u0644\u0647\u0644\u0627\u0645 (SDS-PAGE)\u060c \u0648\u0635\u064f\u0628\u063a\u062a \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0628\u0635\u0628\u063a\u0629 Coomassie Brilliant Blue \u0644\u062a\u0648\u0636\u064a\u062d \u0627\u0644\u0639\u0635\u0627\u0628\u0627\u062a \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u064a\u0629. \u0643\u0645\u0627 \u062c\u0631\u0649 \u0627\u0644\u062a\u062d\u0642\u0642 \u0645\u0646 \u0635\u062d\u0629 \u0627\u0644\u0625\u062f\u062e\u0627\u0644 \u0648\u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062c\u0647\u0627\u0632 Genetic Analyzer System ABI 310 \u0641\u064a \u0647\u064a\u0626\u0629 \u0627\u0644\u0637\u0627\u0642\u0629 \u0627\u0644\u0630\u0631\u064a\u0629 \u0627\u0644\u0633\u0648\u0631\u064a\u0629 \u2013 \u0642\u0633\u0645 \u0627\u0644\u062a\u0642\u0627\u0646\u0629 \u0627\u0644\u062d\u064a\u0648\u064a\u0629\u060c \u0645\u0639 \u0645\u0631\u0626\u0633\u0627\u062a \u0646\u0648\u0639\u064a\u0629 GFP-F\/T7R.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-2133 aligncenter\" src=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/3-300x279.png\" alt=\"\" width=\"355\" height=\"330\" srcset=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/3-300x279.png 300w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/3.png 422w\" sizes=\"auto, (max-width: 355px) 100vw, 355px\" \/><\/p>\n<p><strong>\u062a\u0646\u0642\u064a\u0629 <\/strong><strong>\u0627\u0644\u0625\u064a\u0648\u0645\u064a\u0646\u064a\u062a\u064a\u0646 <\/strong><strong>\u0627\u0644\u0645\u0624\u0634\u0628<\/strong><\/p>\n<p>\u062a\u0645 \u062a\u0646\u0642\u064a\u0629 \u0627\u0644\u0623\u064a\u0648\u0645\u0646\u062a\u064a\u0646 \u0627\u0644\u0645\u0624\u0634\u0628 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062a\u0642\u0646\u064a\u0629 \u0643\u0631\u0648\u0645\u0627\u062a\u0648\u063a\u0631\u0627\u0641\u064a\u0627 \u0627\u0644\u0625\u0644\u0641\u0629 \u00a0\u0627\u0644\u0645\u0639\u062f\u0646\u064a\u0629 (METAL Affinity Chromatography)\u060c \u0628\u0627\u0644\u0627\u0639\u062a\u0645\u0627\u062f \u0639\u0644\u0649 \u0648\u062c\u0648\u062f \u0627\u0644\u0648\u0633\u0645\u0629 \u0633\u062f\u0627\u0633\u064a\u0629 \u0627\u0644\u0647\u0633\u062a\u064a\u062f\u064a\u0646(His-tag6x). \u062a\u0645 \u062a\u0646\u0641\u064a\u0630 \u0639\u0645\u0644\u064a\u0629 \u0627\u0644\u062a\u0646\u0642\u064a\u0629 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062c\u0647\u0627\u0632 <strong>AKTA Prime System<\/strong> \u00a0\u0627\u0644\u0645\u062a\u0648\u0627\u0641\u0631 \u0641\u064a \u0647\u064a\u0626\u0629 \u0627\u0644\u0637\u0627\u0642\u0629 \u0627\u0644\u0630\u0631\u064a\u0629\u060c \u062d\u064a\u062b \u062a\u0645 \u062a\u062d\u0645\u064a\u0644 \u0627\u0644\u062e\u0644\u0627\u0635\u0629 \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0629 \u0627\u0644\u0645\u062d\u062a\u0648\u064a\u0629 \u0639\u0644\u0649 \u0627\u0644\u0625\u064a\u0648\u0645\u0646\u064a\u062a\u064a\u0646 \u0627\u0644\u0645\u0624\u0634\u0628 \u0639\u0644\u0649 \u0639\u0645\u0648\u062f \u00a0\u00a0<strong>Ni-NTA<\/strong> (Qiagen \u0627\u0644\u0645\u0634\u062d\u0648\u0646 \u0628\u0623\u064a\u0648\u0646\u0627\u062a \u0627\u0644\u0646\u064a\u0643\u0644 \u0628\u0633\u0639\u0629 .5 ML<\/p>\n<p>\u0627\u0639\u062a\u0645\u062f\u062a \u0622\u0644\u064a\u0629 \u0627\u0644\u062a\u0646\u0642\u064a\u0629 \u0639\u0644\u0649 \u0627\u0644\u0627\u0631\u062a\u0628\u0627\u0637 \u0627\u0644\u062a\u0641\u0636\u064a\u0644\u064a \u0628\u064a\u0646 \u0627\u0644\u0648\u0633\u0645\u0629 \u0627\u0644\u0647\u064a\u0633\u062a\u064a\u062f\u064a\u0646\u064a\u0629 \u0648\u0623\u064a\u0648\u0646\u0627\u062a \u0627\u0644\u0646\u064a\u0643\u0644 \u0627\u0644\u0645\u0631\u062a\u0628\u0637\u0629 \u0628\u0627\u0644\u0631\u0627\u062a\u0646\u062c\u060c \u0628\u064a\u0646\u0645\u0627 \u0623\u064f\u0632\u064a\u0644\u062a \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u063a\u064a\u0631 \u0627\u0644\u0645\u0631\u062a\u0628\u0637\u0629 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062f\u0627\u0631\u0626\u0629 \u0630\u0627\u062a \u062a\u0631\u0643\u064a\u0632 \u0645\u0646\u062e\u0641\u0636 \u0645\u0646 \u0627\u0644\u0625\u064a\u0645\u064a\u062f\u0627\u0632\u0648\u0644 (\u062f\u0627\u0631\u0626\u0629 \u0627\u0644\u0631\u0628\u0637). \u0648\u062a\u0645 \u0641\u0635\u0644 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0647\u062f\u0641 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062f\u0627\u0631\u0626\u0629 \u0634\u0637\u0641 \u0630\u0627\u062a \u062a\u0631\u0643\u064a\u0632 \u0639\u0627\u0644\u064d \u0645\u0646 \u0627\u0644\u0625\u064a\u0645\u064a\u062f\u0627\u0632\u0648\u0644\u060c \u0628\u0645\u0627 \u064a\u0633\u0645\u062d \u0628\u062a\u062d\u0631\u064a\u0631 \u0627\u0644\u0625\u064a\u0648\u0645\u0646\u064a\u062a\u064a\u0646 \u0645\u0646 \u0627\u0644\u0639\u0645\u0648\u062f.<\/p>\n<p>\u0644\u0627\u062d\u0642\u064b\u0627\u060c \u062a\u0645 \u062a\u062a\u0628\u0639 \u0648\u062c\u0648\u062f \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0641\u0644\u0648\u0631\u064a \u0648\u062a\u0642\u062f\u064a\u0631 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062c\u0647\u0627\u0632 Fluoroskan Ascent (Thermo Scientific)\u060c \u0648\u0630\u0644\u0643 \u0639\u0646\u062f \u0623\u0637\u0648\u0627\u0644 \u0645\u0648\u062c\u064a\u0629 (\u0625\u062b\u0627\u0631\u0629: 485 \u0646\u0627\u0646\u0648\u0645\u062a\u0631 \/ \u0627\u0646\u0628\u0639\u0627\u062b: 538 \u0646\u0627\u0646\u0648\u0645\u062a\u0631)\u060c \u0628\u0645\u0627 \u064a\u062a\u0645\u0627\u0634\u0649 \u0645\u0639 \u0627\u0644\u062e\u0635\u0627\u0626\u0635 \u0627\u0644\u062a\u0623\u0644\u0642\u064a\u0629 \u0644\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0641\u0644\u0648\u0631\u064a \u0627\u0644\u0623\u062e\u0636\u0631. \u062c\u0631\u0649 \u062a\u062d\u062f\u064a\u062f \u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0645\u0646\u0642\u0651\u0649 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0637\u0631\u064a\u0642\u0629 Bradford\u060c \u0648\u0627\u0644\u062a\u064a \u062a\u0639\u062a\u0645\u062f \u0639\u0644\u0649 \u0627\u0631\u062a\u0628\u0627\u0637 \u0635\u0628\u063a\u0629 Coomassie Brilliant Blue \u0628\u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0648\u062d\u062f\u0648\u062b \u0627\u0646\u0632\u064a\u0627\u062d \u0637\u064a\u0641\u064a \u0641\u064a \u0627\u0644\u0627\u0645\u062a\u0635\u0627\u0635 \u064a\u0642\u0627\u0633 \u0639\u0646\u062f \u0637\u0648\u0644 \u0645\u0648\u062c\u064a 595 \u0646\u0627\u0646\u0648\u0645\u062a\u0631. \u0648\u0642\u062f \u062a\u0645 \u0625\u0646\u0634\u0627\u0621 \u0645\u0646\u062d\u0646\u0649 \u0645\u0639\u064a\u0627\u0631\u064a \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062a\u0631\u0627\u0643\u064a\u0632 \u0645\u062e\u062a\u0644\u0641\u0629 \u0645\u0646 \u0623\u0644\u0628\u0648\u0645\u064a\u0646 \u0645\u0635\u0644 \u0627\u0644\u0628\u0642\u0631 (BSA) \u0643\u0645\u0627\u062f\u0629 \u0645\u0631\u062c\u0639\u064a\u0629\u060c \u0648\u0645\u0646 \u062b\u0645 \u062d\u0633\u0627\u0628 \u062a\u0631\u0627\u0643\u064a\u0632 \u0627\u0644\u0639\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u062f\u0631\u0648\u0633\u0629 \u0628\u0627\u0644\u0645\u0642\u0627\u0631\u0646\u0629 \u0645\u0639 \u0627\u0644\u0645\u0646\u062d\u0646\u0649 \u0627\u0644\u0645\u0639\u064a\u0627\u0631\u064a [15]. \u062a\u0645 \u062a\u062d\u0644\u064a\u0644 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0646\u0627\u062a\u062c \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0631\u062d\u0644\u0627\u0646 \u0627\u0644\u0643\u0647\u0631\u0628\u0627\u0626\u064a SDS-PAGE \u0639\u0644\u0649 \u0647\u0644\u0627\u0645\u0629 \u0628\u0648\u0644\u064a \u0623\u0643\u0631\u064a\u0644\u0627\u0645\u064a\u062f 15% \u0645\u0642\u0627\u0631\u0646\u0629\u064b \u0628\u0648\u0627\u0633\u0645 \u0628\u0631\u0648\u062a\u064a\u0646 \u0645\u0639\u064a\u0627\u0631\u064a.<\/p>\n<p><strong>\u0627\u062e\u062a\u0628\u0627\u0631 \u0627\u0644\u0641\u0639\u0627\u0644\u064a\u0629 \u0627\u0644\u0645\u0636\u0627\u062f\u0629 \u0644\u0644\u062c\u0631\u0627\u062b\u064a\u0645<\/strong><\/p>\n<p><strong>\u0627\u0644\u0639\u0632\u0644\u0627\u062a \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0629<\/strong><\/p>\n<p>\u062a\u0645 \u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0623\u0631\u0628\u0639 \u0633\u0644\u0627\u0644\u0627\u062a \u0628\u0643\u062a\u064a\u0631\u064a\u0629 \u0645\u0639\u064a\u0627\u0631\u064a\u0629 \u0644\u062a\u0642\u064a\u064a\u0645 \u0627\u0644\u0641\u0639\u0627\u0644\u064a\u0629 \u0627\u0644\u0645\u0636\u0627\u062f\u0629 \u0644\u0644\u062c\u0631\u0627\u062b\u064a\u0645 \u0644\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0645\u0624\u0634\u0628\u060c \u0648\u0642\u062f \u0634\u0645\u0644\u062a \u0627\u0644\u0633\u0644\u0627\u0644\u0627\u062a \u062c\u0631\u0627\u062b\u064a\u0645 \u0625\u064a\u062c\u0627\u0628\u064a\u0629 \u0627\u0644\u063a\u0631\u0627\u0645 \u0645\u062b\u0644 <em>Staphylococcus aureus<\/em> (ATCC 25923) \u0648<em>Listeria monocytogenes<\/em> (ATCC 19115)\u060c \u0628\u0627\u0644\u0625\u0636\u0627\u0641\u0629 \u0625\u0644\u0649 \u062c\u0631\u0627\u062b\u064a\u0645 \u0633\u0644\u0628\u064a\u0629 \u0627\u0644\u063a\u0631\u0627\u0645 \u0645\u0645\u062b\u0644\u0629 \u0628\u0643\u0644 \u0645\u0646 <em>Salmonella enterica<\/em> (ATCC 14028) \u0648<em>Yersinia enterocolitica<\/em> (ATCC 9610). \u062c\u0631\u0649 \u0627\u0644\u062d\u0635\u0648\u0644 \u0639\u0644\u0649 \u0647\u0630\u0647 \u0627\u0644\u0633\u0644\u0627\u0644\u0627\u062a \u0645\u0646 \u0627\u0644\u0645\u062c\u0645\u0648\u0639\u0629 \u0627\u0644\u0623\u0645\u0631\u064a\u0643\u064a\u0629 \u0644\u0632\u0631\u0627\u0639\u0629 \u0627\u0644\u062e\u0644\u0627\u064a\u0627 (ATCC)\u060c \u0648\u062a\u0645 \u062d\u0641\u0638\u0647\u0627 \u0641\u064a \u062f\u0631\u062c\u0629 \u062d\u0631\u0627\u0631\u0629 \u201380\u00b0C \u0636\u0645\u0646 \u0648\u0633\u0637 \u063a\u0630\u0627\u0626\u064a \u0645\u062f\u0639\u0651\u0645 \u0628\u0646\u0633\u0628\u0629 20% \u0645\u0646 \u0627\u0644\u063a\u0644\u064a\u0633\u0631\u0648\u0644 \u0625\u0644\u0649 \u062d\u064a\u0646 \u0627\u0633\u062a\u062e\u062f\u0627\u0645\u0647\u0627.<\/p>\n<p><strong>\u0627\u062e\u062a\u0628\u0627\u0631 \u0643\u064a\u0631\u0628\u064a-\u0628\u0627\u0648\u0631 \u0644\u0644\u0627\u0646\u062a\u0634\u0627\u0631 \u0628\u0627\u0644\u0642\u0631\u0635<\/strong><strong> (Kirby-Bauer Disk Diffusion Assay)<\/strong><\/p>\n<p>\u062a\u0645 \u062a\u0642\u064a\u064a\u0645 \u0627\u0644\u0641\u0639\u0627\u0644\u064a\u0629 \u0627\u0644\u062d\u064a\u0648\u064a\u0629 \u0644\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0645\u0624\u0634\u0628 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u062e\u062a\u0628\u0627\u0631 \u0643\u064a\u0631\u0628\u064a-\u0628\u0627\u0648\u0631 \u0628\u0637\u0631\u064a\u0642\u0629 \u0627\u0644\u0627\u0646\u062a\u0634\u0627\u0631 \u0628\u0627\u0644\u0642\u0631\u0635\u060c \u0648\u0630\u0644\u0643 \u0648\u0641\u0642\u064b\u0627 \u0644\u0644\u0625\u0631\u0634\u0627\u062f\u0627\u062a \u0627\u0644\u0635\u0627\u062f\u0631\u0629 \u0639\u0646 \u0645\u0639\u0647\u062f \u0627\u0644\u0645\u0639\u0627\u064a\u064a\u0631 \u0627\u0644\u0633\u0631\u064a\u0631\u064a\u0629 \u0648\u0627\u0644\u0645\u062e\u0628\u0631\u064a\u0629 (CLSI) \u062c\u0631\u0649 \u062a\u062d\u0636\u064a\u0631 \u0648\u0633\u0637 \u0645\u0648\u0644\u0631-\u0647\u064a\u0646\u062a\u0648\u0646 \u0627\u0644\u0622\u063a\u0627\u0631 (M\u00fcller-Hinton Agar, MHA) \u0648\u0641\u0642 \u062a\u0639\u0644\u064a\u0645\u0627\u062a \u0627\u0644\u0634\u0631\u0643\u0629 \u0627\u0644\u0645\u0635\u0646\u0651\u0639\u0629\u060c \u062b\u0645 \u0635\u064f\u0628 \u0641\u064a \u0623\u0637\u0628\u0627\u0642 \u0628\u062a\u0631\u064a \u0628\u0633\u0645\u0627\u0643\u0629 \u0645\u062a\u062c\u0627\u0646\u0633\u0629 \u062a\u0628\u0644\u063a \u0646\u062d\u0648 4 \u0645\u0645\u060c \u0628\u0645\u0627 \u064a\u062a\u0648\u0627\u0641\u0642 \u0645\u0639 \u0627\u0644\u0645\u0639\u0627\u064a\u064a\u0631 \u0627\u0644\u0645\u0639\u062a\u0645\u062f\u0629 [16]. \u062a\u0645\u062a \u0632\u0631\u0627\u0639\u0629 \u0645\u0633\u062a\u0639\u0645\u0631\u0627\u062a \u0645\u0641\u0631\u062f\u0629 \u0645\u0646 \u0643\u0644 \u0633\u0644\u0627\u0644\u0629 \u0628\u0643\u062a\u064a\u0631\u064a\u0629 \u0641\u064a \u0623\u0646\u0627\u0628\u064a\u0628 \u062a\u062d\u062a\u0648\u064a \u0639\u0644\u0649 \u0648\u0633\u0637 \u0645\u0648\u0644\u0631-\u0647\u064a\u0646\u062a\u0648\u0646 \u0627\u0644\u0633\u0627\u0626\u0644\u060c \u062b\u0645 \u062d\u064f\u0636\u0646\u062a \u0639\u0646\u062f \u062f\u0631\u062c\u0629 \u062d\u0631\u0627\u0631\u0629 37\u00b0C \u0644\u0645\u062f\u0629 18 \u0633\u0627\u0639\u0629 \u0645\u0639 \u0627\u0644\u062a\u062d\u0631\u064a\u0643 \u0627\u0644\u0645\u0633\u062a\u0645\u0631. \u0628\u0639\u062f \u0627\u0644\u062d\u0636\u0627\u0646\u0629\u060c \u062a\u0645 \u0642\u064a\u0627\u0633 \u0639\u0643\u0627\u0631\u0629 \u0627\u0644\u0645\u0639\u0644\u0642\u0627\u062a \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0629 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062c\u0647\u0627\u0632 \u0627\u0644\u0645\u0637\u064a\u0627\u0641 \u0627\u0644\u0636\u0648\u0626\u064a (spectrophotometer) \u0639\u0644\u0649 \u0637\u0648\u0644 \u0645\u0648\u062c\u0647 600 \u0646\u0627\u0646\u0648\u0645\u062a\u0631\u060c \u0648\u0630\u0644\u0643 \u0644\u0636\u0628\u0637 \u0627\u0644\u0643\u062b\u0627\u0641\u0629 \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0629 \u0628\u0645\u0627 \u064a\u062a\u0648\u0627\u0641\u0642 \u0645\u0639 McFarland standard 0.5\u060c \u0648\u0627\u0644\u0630\u064a \u064a\u0639\u0627\u062f\u0644 \u062a\u0642\u0631\u064a\u0628\u064b\u0627 1.5\u00d710\u2078 \u0648\u062d\u062f\u0629 \u062a\u0634\u0643\u064a\u0644 \u0645\u0633\u062a\u0639\u0645\u0631\u0629 \u0644\u0643\u0644 \u0645\u0644\u064a\u0644\u062a\u0631(CFU\/mL) .<br \/>\n\u062a\u0645 \u062a\u0644\u0642\u064a\u062d \u0633\u0637\u062d \u0648\u0633\u0637 \u0627\u0644\u0622\u063a\u0627\u0631 \u0627\u0644\u0645\u062d\u0636\u0651\u0631 \u0645\u0633\u0628\u0642\u064b\u0627 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0645\u0633\u062d\u0629 \u0645\u0639\u0642\u0651\u0645\u0629 \u0645\u063a\u0645\u0648\u0633\u0629 \u0641\u064a \u0627\u0644\u0645\u064f\u0639\u0644\u0651\u0642 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\u0627\u0644\u062d\u0627\u0636\u0646\u0629 \u0639\u0646\u062f \u062f\u0631\u062c\u0629 \u062d\u0631\u0627\u0631\u0629 37\u00b0\u0645 \u0644\u0644\u0645\u062f\u0629 24 \u0633\u0627\u0639\u0629. \u062b\u0645 \u062a\u0645 \u0642\u064a\u0627\u0633 \u0623\u0642\u0637\u0627\u0631 \u0645\u0646\u0627\u0637\u0642 \u062a\u062b\u0628\u064a\u0637 \u0627\u0644\u0646\u0645\u0648 \u0648\u062a\u0645 \u062a\u062d\u0644\u064a\u0644 \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0644\u062a\u062d\u062f\u064a\u062f \u0627\u0644\u0641\u0639\u0627\u0644\u064a\u0629 \u0627\u0644\u0645\u0636\u0627\u062f\u0629 \u0644\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0627 \u0644\u0644\u0645\u0631\u0643\u0628 \u0627\u0644\u0645\u062f\u0631\u0648\u0633.<\/p>\n<p><strong>\u0627\u0644\u062a\u062d\u0644\u064a\u0644 \u0627\u0644\u0627\u062d\u0635\u0627\u0626\u064a <\/strong><\/p>\n<p>\u062a\u0645 \u062a\u062d\u0644\u064a\u0644 \u0627\u0644\u0628\u064a\u0627\u0646\u0627\u062a \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0628\u0631\u0646\u0627\u0645\u062c SPSS \u00a0\u0627\u0644\u0625\u0635\u062f\u0627\u0631 26\u060c \u0648\u0630\u0644\u0643 \u0628\u0627\u0639\u062a\u0645\u0627\u062f \u0627\u062e\u062a\u0628\u0627\u0631 \u062a\u062d\u0644\u064a\u0644 \u0627\u0644\u062a\u0628\u0627\u064a\u0646 \u0627\u0644\u0623\u062d\u0627\u062f\u064a (One-way \u00a0ANOVA) \u00a0\u0645\u062a\u0628\u0648\u0639\u064b\u0627 \u0628\u0627\u062e\u062a\u0628\u0627\u0631 \u062a\u0648\u0643\u064a \u0644\u0644\u0645\u0642\u0627\u0631\u0646\u0627\u062a \u0627\u0644\u0628\u0639\u062f\u064a\u0629 (Tukey\u2019s post-hoc test) \u00a0\u0644\u062a\u062d\u062f\u064a\u062f \u0627\u0644\u062f\u0644\u0627\u0644\u0629 \u0627\u0644\u0625\u062d\u0635\u0627\u0626\u064a\u0629 \u0628\u064a\u0646 \u0627\u0644\u0645\u062c\u0645\u0648\u0639\u0627\u062a\u060c \u0628\u0627\u0639\u062a\u0628\u0627\u0631 \u0642\u064a\u0645\u0629 <em>P &lt; 0.05<\/em> \u00a0\u0645\u0624\u0634\u0631\u064b\u0627 \u0639\u0644\u0649 \u0641\u0631\u0642 \u0645\u0639\u0646\u0648\u064a \u0625\u062d\u0635\u0627\u0626\u064a.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u0627\u0644\u0646\u062a\u0627\u0626\u062c <\/strong><strong>Results<\/strong><\/p>\n<p><strong>\u0625\u0646\u062a\u0627\u062c \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0645\u0624\u0634\u0628<\/strong><\/p>\n<p>\u062a\u0645 \u0627\u0644\u062a\u062d\u0642\u0642 \u0645\u0646 \u0646\u062c\u0627\u062d \u0625\u062f\u062e\u0627\u0644 \u062c\u064a\u0646 Eumenitin \u0627\u0644\u0645\u0635\u0645\u0645 \u0635\u0646\u0627\u0639\u064a\u064b\u0627 \u0625\u0644\u0649 \u0646\u0638\u0627\u0645 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a E. coli \u0639\u0628\u0631 \u0639\u062f\u0629 \u0645\u0631\u0627\u062d\u0644.\u0623\u0638\u0647\u0631\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0627\u0644\u0623\u0648\u0644\u064a\u0629 \u0644\u062a\u0641\u0627\u0639\u0644 \u0627\u0644\u0628\u0648\u0644\u064a\u0645\u064a\u0631\u0627\u0632 \u0627\u0644\u0645\u062a\u0633\u0644\u0633\u0644 (PCR) \u0644\u0644\u0645\u0633\u062a\u0639\u0645\u0631\u0627\u062a \u0627\u0644\u0645\u062d\u0648\u0651\u0631\u0629 \u0634\u0631\u064a\u0637\u064b\u0627 \u0648\u0627\u0636\u062d\u064b\u0627 \u0639\u0644\u0649 \u0627\u0644\u0647\u0644\u0627\u0645 \u0627\u0644\u0643\u0647\u0631\u0628\u0627\u0626\u064a \u0639\u0646\u062f \u062d\u062c\u0645 \u064a\u0642\u0627\u0631\u0628 286 \u0632\u0648\u062c\u064b\u0627 \u0642\u0627\u0639\u062f\u064a\u064b\u0627\u060c \u0648\u0647\u0648 \u0645\u0627 \u064a\u062a\u0648\u0627\u0641\u0642 \u0645\u0639 \u0627\u0644\u062d\u062c\u0645 \u0627\u0644\u0645\u062a\u0648\u0642\u0639 \u0644\u0644\u0642\u0637\u0639\u0629 \u0627\u0644\u0645\u064f\u0633\u062a\u0647\u062f\u0641\u0629 (\u0627\u0644\u0634\u0643\u0644 3).<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-2134 aligncenter\" src=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/4-300x198.png\" alt=\"\" width=\"359\" height=\"237\" srcset=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/4-300x198.png 300w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/4.png 372w\" sizes=\"auto, (max-width: 359px) 100vw, 359px\" \/><\/p>\n<p style=\"text-align: center;\"><strong>\u0627\u0644\u0634\u0643\u0644 (3): \u062a\u0641\u0627\u0639\u0644 PCR \u0639\u0644\u0649 \u0645\u0633\u062a\u0639\u0645\u0631\u0627\u062a \u0628\u0643\u062a\u064a\u0631\u064a\u0629 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0628\u0627\u062f\u0626\u0627\u062a T7F\/T7R \u0628\u0639\u062f \u062a\u062d\u0648\u064a\u0644 \u062e\u0644\u0627\u064a\u0627<em>E. Coli<\/em> \u0628\u0628\u0644\u0627\u0632\u0645\u064a\u062f pRSET-sfGFP. \u00a0\u062a\u0645 \u062a\u062d\u0644\u064a\u0644 \u0646\u0648\u0627\u062a\u062c PCR \u0639\u0644\u0649 \u0647\u0644\u0627\u0645 \u0623\u063a\u0627\u0631\u0648\u0632 \u0628\u062a\u0631\u0643\u064a\u0632 1.5%. \u0627\u0633\u062a\u064f\u062e\u062f\u0645 \u0648\u0627\u0633\u0645 \u0623\u0637\u0648\u0627\u0644 \u0645\u0639\u064a\u0627\u0631\u064a\u0629 (100 bp DNA ladder\u060c Promega) \u00a0\u0628\u0645\u062f\u0649 100\u20131500 bp. \u064a\u064f\u0638\u0647\u0631 \u0627\u0644\u0628\u0626\u0631 (1) \u0627\u0644\u0634\u0627\u0647\u062f \u0627\u0644\u0633\u0644\u0628\u064a (\u0627\u0644\u0628\u0644\u0627\u0632\u0645\u064a\u062f \u0641\u0642\u0637) \u062f\u0648\u0646 \u0638\u0647\u0648\u0631 \u0634\u0631\u064a\u0637 \u0628\u0627\u0644\u062d\u062c\u0645 \u0627\u0644\u0645\u062a\u0648\u0642\u0639\u060c \u0628\u064a\u0646\u0645\u0627 \u064a\u064f\u0638\u0647\u0631 \u0627\u0644\u0628\u0626\u0631 (2) \u0634\u062f\u0641\u0629 \u0627\u0644\u0625\u064a\u0648\u0645\u064a\u0646\u064a\u062a\u064a\u0646 \u0628\u0627\u0644\u062d\u062c\u0645 \u0627\u0644\u0645\u062a\u0648\u0642\u0639 (bp~286)<\/strong><\/p>\n<p>\u0639\u0632\u0632 \u062a\u062d\u0644\u064a\u0644 \u0627\u0644\u062a\u0642\u0637\u064a\u0639 \u0627\u0644\u0625\u0646\u0632\u064a\u0645\u064a \u0644\u0644\u0628\u0644\u0627\u0632\u0645\u064a\u062f \u0627\u0644\u0645\u0624\u0634\u0628 \u0647\u0630\u0647 \u0627\u0644\u0646\u062a\u064a\u062c\u0629\u060c \u062d\u064a\u062b \u0623\u0638\u0647\u0631\u062a \u0627\u0644\u0639\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u062d\u0644\u0651\u0644\u0629 \u062d\u0632\u0645\u0629 \u0645\u0645\u064a\u0632\u0629 \u0628\u0627\u0644\u062d\u062c\u0645 \u0627\u0644\u0645\u062a\u0648\u0642\u0639 \u0628\u0639\u062f \u0627\u0644\u0647\u0636\u0645 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0625\u0646\u0632\u064a\u0645\u064a ApaI \u0648HindIII\u060c \u0645\u0645\u0627 \u064a\u0634\u064a\u0631 \u0625\u0644\u0649 \u0627\u0643\u062a\u0645\u0627\u0644 \u0639\u0645\u0644\u064a\u0629 \u0627\u0644\u0625\u062f\u062e\u0627\u0644 \u0627\u0644\u062c\u064a\u0646\u064a. \u0627\u0644\u0634\u0643\u0644 (4)<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2135 aligncenter\" src=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/5.png\" alt=\"\" width=\"248\" height=\"276\" \/><\/p>\n<p style=\"text-align: center;\"><strong>\u0627\u0644\u0634\u0643\u0644 (4): \u062d\u0632\u0645\u0629<\/strong> <strong>\u0627\u0644\u0625\u064a\u0648\u0645\u0646\u064a\u062a\u064a\u0646 \u0627\u0644\u0645\u0624\u0634\u0628 <\/strong><strong>\u0628\u0627\u0644\u062d\u062c\u0645 \u0627\u0644\u0645\u062a\u0648\u0642\u0639 <\/strong><strong>\u00a0<\/strong><strong>1085 ~bp <\/strong><strong>\u0628\u0639\u062f \u0627\u0644\u0647\u0636\u0645 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0625\u0646\u0632\u064a\u0645\u064a<\/strong><strong> ApaI <\/strong><strong>\u0648<\/strong><strong>.HindIII<\/strong><\/p>\n<p>\u0643\u0645\u0627 \u0628\u064a\u0651\u0646 \u062a\u062d\u0644\u064a\u0644 \u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u062f\u0646\u0627 (DNA sequencing) \u062a\u0637\u0627\u0628\u0642\u064b\u0627 \u0643\u0627\u0645\u0644\u064b\u0627 \u0628\u0646\u0633\u0628\u0629 100% \u0628\u064a\u0646 \u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u062c\u064a\u0646 \u0627\u0644\u0645\u064f\u062f\u062e\u0644 \u0648\u0627\u0644\u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u0645\u064f\u0635\u0645\u0645 \u0628\u0639\u062f \u062a\u062d\u0633\u064a\u0646 \u0627\u0644\u0643\u0648\u062f\u0648\u0646\u0627\u062a\u060c \u062f\u0648\u0646 \u0648\u062c\u0648\u062f \u0623\u064a \u0637\u0641\u0631\u0627\u062a \u0646\u0642\u0637\u064a\u0629 \u0623\u0648 \u062d\u0630\u0641\u060c \u0645\u0645\u0627 \u064a\u0624\u0643\u062f \u062f\u0642\u0629 \u0627\u0644\u0625\u062f\u062e\u0627\u0644 \u0648\u0645\u0648\u062b\u0648\u0642\u064a\u062a\u0647. \u062a\u0645 \u062a\u062a\u0628\u0651\u0639 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u0645\u0624\u0634\u0628 \u0644\u0628\u0646\u064a\u0629 Eumenitin-sfGFP \u00a0\u0641\u064a \u062e\u0644\u0627\u064a\u0627 E. coli \u00a0\u0645\u0646 \u0646\u0645\u0637 BL21 (Gold) \u0628\u0639\u062f \u0627\u0644\u062a\u062d\u0631\u064a\u0636 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0645\u0627\u062f\u0629 IPTG\u060c \u0648\u0630\u0644\u0643 \u0628\u0627\u0644\u0627\u0639\u062a\u0645\u0627\u062f \u0639\u0644\u0649 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0641\u0644\u0648\u0631\u064a \u0627\u0644\u0623\u062e\u0636\u0631 (GFP) \u0627\u0644\u0645\u064f\u062f\u0645\u062c \u0643\u0645\u064f\u0639\u0644\u0645 \u0628\u0635\u0631\u064a. \u0644\u0648\u062d\u0638 \u062a\u0648\u0647\u0651\u062c \u0641\u0644\u0648\u0631\u064a \u0623\u062e\u0636\u0631 \u0648\u0627\u0636\u062d \u0641\u064a \u0627\u0644\u0645\u0633\u062a\u0639\u0645\u0631\u0627\u062a \u0627\u0644\u0645\u062d\u0631\u0651\u0636\u0629 \u0628\u0639\u062f \u0641\u062a\u0631\u0629 \u062d\u0636\u0646 \u0627\u0633\u062a\u0645\u0631\u062a 16 \u0633\u0627\u0639\u0629 \u0639\u0646\u062f \u062f\u0631\u062c\u0629 \u062d\u0631\u0627\u0631\u0629 28\u202f\u00b0\u0645\u060c \u0645\u0645\u0627 \u064a\u062f\u0644 \u0639\u0644\u0649 \u0646\u062c\u0627\u062d \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u064a \u0627\u0644\u0645\u0633\u062a\u0647\u062f\u0641 \u0648\u064a\u0624\u0643\u062f \u0643\u0641\u0627\u0621\u0629 \u0646\u0638\u0627\u0645 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u0645\u0633\u062a\u062e\u062f\u0645. \u064a\u0628\u064a\u0646 \u0627\u0644\u0634\u0643\u0644 (5) \u0627\u0644\u0631\u062d\u0644\u0627\u0646 \u0627\u0644\u0643\u0647\u0631\u0628\u0627\u0626\u064a (SDS-PAGE) \u0623\u0646\u0647 \u0628\u0639\u062f \u0645\u0631\u0648\u0631 16 \u0633\u0627\u0639\u0629 \u0639\u0644\u0649 \u062a\u062d\u0631\u064a\u0636 IPTG \u0627\u0644\u0628\u0626\u0631 (4)\u060c \u0644\u0648\u062d\u0638 \u062a\u0639\u0628\u064a\u0631 \u0645\u0644\u062d\u0648\u0638 \u0644\u0628\u0631\u0648\u062a\u064a\u0646 sfGFP-Eumenitin \u00a0\u0628\u0648\u0632\u0646 \u062c\u0632\u064a\u0626\u064a 38 \u0643\u064a\u0644\u0648\u062f\u0627\u0644\u062a\u0648\u0646 \u0641\u064a \u062d\u064a\u0646 \u0644\u0645 \u064a\u062a\u0645\u00a0 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0639\u0646\u0647 \u0639\u0646\u062f \u0627\u0644\u0632\u0645\u0646 0 \u0627\u0644\u0628\u0626\u0631 (3) \u0648 \u0646\u0644\u0627\u062d\u0638 \u0628\u062f\u0621 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0628\u0639\u062f 6 \u0633\u0627\u0639\u0627\u062a \u0627\u0644\u0628\u0626\u0631 (2).<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-2136 aligncenter\" src=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/6-300x212.png\" alt=\"\" width=\"300\" height=\"212\" srcset=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/6-300x212.png 300w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/6.png 382w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/p>\n<p style=\"text-align: center;\"><strong>\u0627\u0644\u0634\u0643\u0644 (5): \u0647\u0644\u0627\u0645\u0629 \u0639\u062f\u064a\u062f \u0627\u0644\u0623\u0643\u0631\u064a\u0644\u0627\u0645\u064a\u062f \u0628\u062a\u0631\u0643\u064a\u0632 15%\u060c \u0627\u0644\u0645\u0635\u0628\u0648\u063a\u0629 \u0628\u0635\u0628\u063a\u0629 \u0627\u0644\u0643\u0648\u0645\u0627\u0633\u064a \u0627\u0644\u0632\u0631\u0642\u0627\u0621. \u064a\u0645\u062b\u0644 \u00a0\u0627\u0644\u0628\u0626\u0631 M \u00a0\u0627\u0644\u0648\u0627\u0633\u0645 \u0627\u0644\u0645\u0639\u064a\u0627\u0631\u064a \u0644\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a\u060c \u0627\u0644\u0628\u0626\u06313 (T0) \u064a\u0645\u062b\u0644 \u062e\u0644\u0627\u0635\u0629 \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0627 \u0642\u0628\u0644 \u0627\u0644\u062a\u062d\u0631\u064a\u0636\u060c \u0627\u0644\u0628\u0626\u0631 4 (T16) \u064a\u062d\u062a\u0648\u064a \u0639\u0644\u0649 \u062e\u0644\u0627\u0635\u0629 \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0627 \u0628\u0639\u062f 16 \u0633\u0627\u0639\u0629 \u0645\u0646 \u0627\u0644\u062a\u062d\u0631\u064a\u0636\u060c \u0648\u062a\u064f\u0628\u064a\u0646\u0631 \u0638\u0647\u0648\u0631 \u0639\u062f\u0629 \u0639\u0635\u0627\u0628\u0627\u062a\u060c \u0645\u0646 \u0628\u064a\u0646\u0647\u0627 \u0639\u0635\u0627\u0628\u0629 \u062a\u0642\u0627\u0631\u0628 \u0648\u0632\u0646\u0647\u0627 \u0627\u0644\u062c\u0632\u064a\u0626\u064a \u00a038kDa \u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0645\u0633\u062a\u0647\u062f\u0641 \u0628\u062f\u0648\u0646 \u062a\u0646\u0642\u064a\u0629\u060c \u0646\u0644\u0627\u062d\u0638 \u0641\u064a \u0627\u0644\u0628\u0626\u0631 2(T6) \u0627\u062b\u0631 \u0644\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0628\u0639\u062f 6 \u0633\u0627\u0639\u0627\u062a \u0645\u0646 \u0627\u0644\u062a\u062d\u0631\u064a\u0636 \u062f\u0644\u064a\u0644 \u0628\u062f\u0621 \u0639\u0645\u0644\u064a\u0629 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u0628\u0626\u0631 1 \u0645\u0643\u0631\u0631 \u0644\u0639\u064a\u0646\u0629 T6.<\/strong><\/p>\n<p><strong>\u062a\u0646\u0642\u064a\u0629 \u0627\u0644\u0625\u064a\u0648\u0645\u0646\u064a\u062a\u064a\u0646 \u0627\u0644\u0645\u0623\u0634\u0648\u0628<\/strong><\/p>\n<p>\u062a\u0645 \u0627\u0633\u062a\u062e\u0644\u0627\u0635 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0645\u0646\u062a\u062c \u0641\u064a \u062e\u0644\u0627\u064a\u0627 \u0627\u0644\u0628\u0643\u062a\u064a\u0631\u064a\u0627 \u0645\u0646 \u0627\u0644\u0633\u064a\u062a\u0648\u0628\u0644\u0627\u0632\u0645 \u0628\u0639\u062f 16 \u0633\u0627\u0639\u0629 \u0645\u0646 \u0627\u0644\u062a\u062d\u0631\u064a\u0636\u060c \u062a\u0644\u0627 \u0630\u0644\u0643 \u062a\u0646\u0642\u064a\u0629 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0645\u0624\u0634\u0628 \u00a0\u00a0\u00a0DECO-sfGFP \u00a0\u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0639\u0645\u0648\u062f \u0643\u0631\u0648\u0645\u0627\u062a\u0648\u063a\u0631\u0627\u0641\u064a\u0627 \u0627\u0644\u0625\u0644\u0641\u0629 \u0627\u0644\u0645\u0639\u062f\u0646\u064a\u0629 \u0627\u0644\u0645\u0634\u062d\u0648\u0646 \u0628\u0623\u064a\u0648\u0646\u0627\u062a \u0627\u0644\u0646\u064a\u0643\u0644 \u0639\u0644\u0649 \u062c\u0647\u0627\u0632 AKTA Prime System. \u0623\u062a\u0627\u062d \u0643\u0627\u0634\u0641 \u0627\u0644\u0623\u0634\u0639\u0629 \u0641\u0648\u0642 \u0627\u0644\u0628\u0646\u0641\u0633\u062c\u064a\u0629 (UV) \u0627\u0644\u0645\u062f\u0645\u062c \u0641\u064a \u0647\u0630\u0627 \u0627\u0644\u0646\u0638\u0627\u0645 \u0627\u0644\u0645\u0631\u0627\u0642\u0628\u0629 \u0627\u0644\u0641\u0648\u0631\u064a\u0629 \u0648\u0627\u0644\u0645\u0628\u0627\u0634\u0631\u0629 \u0644\u062c\u0645\u064a\u0639 \u0645\u0631\u0627\u062d\u0644 \u0639\u0645\u0644\u064a\u0629 \u0627\u0644\u062a\u0646\u0642\u064a\u0629 (\u0627\u0644\u0634\u0643\u0644 6) \u0648 \u062c\u0645\u0639 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0645\u0624\u0634\u0628.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-2137 aligncenter\" src=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/7-300x190.png\" alt=\"\" width=\"321\" height=\"203\" srcset=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/7-300x190.png 300w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/7.png 387w\" sizes=\"auto, (max-width: 321px) 100vw, 321px\" \/><\/p>\n<p style=\"text-align: center;\">\u0627<strong>\u0644\u0634\u0643\u0644 (<\/strong><strong>6<\/strong><strong>): \u062a\u0646\u0642\u064a\u0629 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0645\u0623\u0634\u0648\u0628 <\/strong><strong>Eumen-sfGFP<\/strong><strong> \u0628\u0639\u0645\u0648\u062f \u0643\u0631\u0648\u0645\u0627\u062a\u0648\u063a\u0631\u0627\u0641\u064a\u0627 \u0627\u0644\u0625\u0644\u0641\u0629 \u0627\u0644\u0634\u0627\u0631\u062f\u064a\u0629 \u0644\u0634\u0648\u0627\u0631\u062f \u0627\u0644\u0646\u064a\u0643\u0644<\/strong><strong>.<\/strong><\/p>\n<p>\u062a\u0645 \u062a\u062d\u0644\u064a\u0644 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0645\u0646\u0642\u0649 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062a\u0642\u0646\u064a\u0629 SDS-PAGE \u0644\u0648\u062d\u0638 \u0648\u062c\u0648\u062f \u0639\u0635\u0627\u0628\u0629 \u0628\u0631\u0648\u062a\u064a\u0646\u064a\u0629 \u0648\u0627\u0636\u062d\u0629 \u0639\u0646\u062f \u0648\u0632\u0646 \u062c\u0632\u064a\u0626\u064a \u064a\u064f\u0642\u062f\u0651\u0631 \u0628\u062d\u0648\u0627\u0644\u064a 38 \u0643\u064a\u0644\u0648\u062f\u0627\u0644\u062a\u0648\u0646\u060c \u0623\u0638\u0647\u0631 \u062a\u062d\u0644\u064a\u0644 SDS-PAGE \u0639\u0635\u0627\u0628\u0629 \u0628\u0631\u0648\u062a\u064a\u0646\u064a\u0629 \u0628\u0627\u0644\u062d\u062c\u0645 ~38 \u0643\u064a\u0644\u0648\u062f\u0627\u0644\u062a\u0648\u0646.<\/p>\n<p><strong>\u062a\u0642\u064a\u064a\u0645 \u0627\u0644\u0641\u0639\u0627\u0644\u064a\u0629 \u0627\u0644\u0645\u0636\u0627\u062f\u0629 \u0644\u0644\u062c\u0631\u0627\u062b\u064a\u0645 \u0644\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0645\u0624\u0634\u0628<\/strong><\/p>\n<p>\u062a\u0645 \u062a\u0642\u064a\u064a\u0645 \u0627\u0644\u0646\u0634\u0627\u0637 \u0627\u0644\u0645\u0636\u0627\u062f \u0644\u0644\u062c\u0631\u0627\u062b\u064a\u0645 \u0644\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0645\u0624\u0634\u0628 \u0645\u0646 \u0646\u0648\u0639 \u0627\u0644\u0625\u064a\u0648\u0645\u064a\u0646\u062a\u064a\u0646 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u062e\u062a\u0628\u0627\u0631 \u0643\u064a\u0631\u0628\u064a-\u0628\u0627\u0648\u0631 \u0636\u062f \u0623\u0631\u0628\u0639 \u0633\u0644\u0627\u0644\u0627\u062a \u062c\u0631\u062b\u0648\u0645\u064a\u0629 \u0645\u0639\u064a\u0627\u0631\u064a\u0629 \u0627\u0644\u062c\u062f\u0648\u0644 (2). \u0623\u0638\u0647\u0631\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0623\u0646 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0645\u0624\u0634\u0628 \u0623\u062d\u062f\u062b \u0645\u0646\u0627\u0637\u0642 \u062a\u062b\u0628\u064a\u0637 \u0648\u0627\u0636\u062d\u0629 \u0641\u064a \u0627\u0644\u0633\u0644\u0627\u0644\u062a\u064a\u0646 \u0625\u064a\u062c\u0627\u0628\u064a\u062a\u064e\u064a \u0627\u0644\u063a\u0631\u0627\u0645 \u060c \u062d\u064a\u062b \u0628\u0644\u063a \u0645\u062a\u0648\u0633\u0637 \u0642\u0637\u0631 \u0645\u0646\u0637\u0642\u0629 \u0627\u0644\u062a\u062b\u0628\u064a\u0637 \u0636\u062f \u00a0<em>S.aureus<\/em> 17.3 \u00b1 0.5 \u0645\u0645\u060c \u0648\u0643\u0627\u0646 <em>L.monocytogenes<\/em> 15.9 \u00a0\u00b1 0.7 \u0645\u0645 \u0627\u0644\u0634\u0643\u0644 (7). \u0623\u0645\u0627 \u0641\u064a \u0627\u0644\u0633\u0644\u0627\u0644\u0627\u062a \u0633\u0644\u0628\u064a\u0629 \u0627\u0644\u063a\u0631\u0627\u0645\u060c \u0641\u0642\u062f \u0644\u0648\u062d\u0638 \u0646\u0634\u0627\u0637 \u0645\u0636\u0627\u062f \u0644\u0644\u0645\u064a\u0643\u0631\u0648\u0628\u0627\u062a \u0648\u0644\u0643\u0646 \u0628\u062f\u0631\u062c\u0629 \u0623\u0642\u0644\u060c \u062d\u064a\u062b \u0628\u0644\u063a \u0645\u062a\u0648\u0633\u0637 \u0642\u0637\u0631 \u0645\u0646\u0637\u0642\u0629 \u0627\u0644\u062a\u062b\u0628\u064a\u0637 \u0636\u062f <em>S. enterica<\/em> 11.2 \u00b1 0.6 \u0645\u0645\u060c \u0648\u0636\u062f <em>Y. enterocolitica<\/em> 10.5 \u00b1 0.8 \u00a0\u0645\u0645\u060c \u0645\u0639 \u062a\u0633\u062c\u064a\u0644 \u0628\u0639\u0636 \u062d\u0627\u0644\u0627\u062a \u0627\u0644\u0646\u0645\u0648 \u0627\u0644\u062c\u0632\u0626\u064a \u0642\u0631\u0628 \u062d\u062f\u0648\u062f \u0645\u0646\u0627\u0637\u0642 \u0627\u0644\u062a\u062b\u0628\u064a\u0637.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-2139 aligncenter\" src=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/9-289x300.png\" alt=\"\" width=\"308\" height=\"320\" srcset=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/9-289x300.png 289w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/9.png 387w\" sizes=\"auto, (max-width: 308px) 100vw, 308px\" \/><\/p>\n<p style=\"text-align: center;\"><strong>\u0627\u0644\u0634\u0643\u0644 (7): \u062a\u0642\u064a\u064a\u0645 \u0627\u0644\u0641\u0639\u0627\u0644\u064a\u0629 \u0627\u0644\u0645\u0636\u0627\u062f\u0629 \u0644\u0644\u062c\u0631\u0627\u062b\u064a\u0645 \u0644\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0645\u0624\u0634\u0628<\/strong><strong> Eumen-sfGFP<\/strong> <strong>\u00a0\u0636\u062f <\/strong><strong>\u00a0<\/strong><strong>\u00a0<\/strong><strong><em>Listeria monocytogenes<\/em><\/strong><strong>\u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u062e\u062a\u0628\u0627\u0631 \u0643\u064a\u0631\u0628\u064a-\u0628\u0627\u0648\u0631 \u062a\u064f\u0638\u0647\u0631 \u0627\u0644\u0635\u0648\u0631\u0629 \u0645\u0646\u0627\u0637\u0642 \u062a\u062b\u0628\u064a\u0637 \u0648\u0627\u0636\u062d\u0629: <\/strong><strong>(A)<\/strong> <strong>\u00a0<\/strong><strong>\u0628\u0628\u062a\u064a\u062f \u0639\u064a\u0627\u0631\u064a <\/strong><strong>(C) <\/strong><strong>\u00a0\u0627\u0644\u0645\u062d\u0645\u0651\u0644 \u0628\u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0645\u0624\u0634\u0628 <\/strong><strong>\u00a0(B)<\/strong><strong> \u0648\u0627\u0644\u0634\u0627\u0647\u062f \u0627\u0644\u0633\u0644\u0628\u064a<\/strong> <strong>\u0645\u062d\u0644\u0648\u0644 \u062f\u0627\u0631\u0626\u0629 \u0641\u0642\u0637<\/strong><strong>(D) <\/strong><strong>\u0627\u0644\u0645\u0636\u0627\u062f \u0627\u0644\u062d\u064a\u0648\u064a \u0627\u0644\u0645\u0631\u062c\u0639\u064a \u0643\u0627\u0631\u0628\u0646\u0633\u064a\u0644\u064a\u0646<\/strong>.<\/p>\n<p>\u0623\u0638\u0647\u0631\u062a \u0645\u0642\u0627\u0631\u0646\u0629 \u0627\u0644\u0641\u0639\u0627\u0644\u064a\u0629 \u0623\u0646 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0645\u0624\u0634\u0628 \u062d\u0627\u0641\u0638 \u0639\u0644\u0649 \u0645\u0633\u062a\u0648\u0649 \u0645\u0642\u0627\u0631\u0628 \u0644\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0646\u0642\u064a \u0627\u0644\u062a\u062c\u0627\u0631\u064a (Novoprolabs)\u060c \u062d\u064a\u062b \u0643\u0627\u0646\u062a \u0641\u0631\u0648\u0642 \u0623\u0642\u0637\u0627\u0631 \u0645\u0646\u0627\u0637\u0642 \u0627\u0644\u062a\u062b\u0628\u064a\u0637 \u063a\u064a\u0631 \u0645\u0639\u0646\u0648\u064a\u0629 \u0625\u062d\u0635\u0627\u0626\u064a\u064b\u0627 (P &gt; 0.05) \u0641\u064a \u0645\u0639\u0638\u0645 \u0627\u0644\u0633\u0644\u0627\u0644\u0627\u062a. \u0641\u064a \u0627\u0644\u0645\u0642\u0627\u0628\u0644\u060c \u0623\u0639\u0637\u062a \u0627\u0644\u0623\u0642\u0631\u0627\u0635 \u0627\u0644\u0645\u062d\u0645\u0651\u0644\u0629 \u0628\u0627\u0644\u0645\u0636\u0627\u062f\u0627\u062a \u0627\u0644\u062d\u064a\u0648\u064a\u0629 \u0627\u0644\u0645\u0631\u062c\u0639\u064a\u0629 (\u0643\u0627\u0631\u0628\u0646\u0633\u064a\u0644\u064a\u0646 \u0623\u0648 \u0641\u0627\u0646\u0643\u0648\u0645\u0627\u064a\u0633\u064a\u0646) \u0645\u0646\u0627\u0637\u0642 \u062a\u062b\u0628\u064a\u0637 \u0623\u0643\u0628\u0631 \u062a\u0631\u0627\u0648\u062d\u062a \u0628\u064a\u0646 20.1 \u00b1 0.9 \u0645\u0645 \u064823.4 \u00b1 1.1 \u0645\u0645. \u0623\u0645\u0627 \u0623\u0642\u0631\u0627\u0635 \u0627\u0644\u0634\u0627\u0647\u062f \u0627\u0644\u0633\u0644\u0628\u064a (\u0645\u062d\u0644\u0648\u0644 \u0627\u0644\u062f\u0627\u0631\u0626\u0629 \u0641\u0642\u0637) \u0641\u0644\u0645 \u062a\u064f\u0638\u0647\u0631 \u0623\u064a \u0645\u0646\u0637\u0642\u0629 \u062a\u062b\u0628\u064a\u0637\u060c \u0645\u0645\u0627 \u064a\u0634\u064a\u0631 \u0625\u0644\u0649 \u0623\u0646 \u0627\u0644\u0646\u0634\u0627\u0637 \u0627\u0644\u0645\u0636\u0627\u062f \u064a\u0639\u0648\u062f \u062d\u0635\u0631\u064a\u064b\u0627 \u0644\u0644\u0628\u0628\u062a\u064a\u062f. \u062c\u062f\u0648\u0644 (2).<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-2138 aligncenter\" src=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/8-300x106.png\" alt=\"\" width=\"447\" height=\"158\" srcset=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/8-300x106.png 300w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/8-768x271.png 768w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2026\/02\/8.png 843w\" sizes=\"auto, (max-width: 447px) 100vw, 447px\" \/><\/p>\n<p><strong>\u0627\u0644\u0645\u0646\u0627\u0642\u0634\u0629 <\/strong><strong>Discussion<\/strong><\/p>\n<p>\u0623\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u062c\u064a\u0646\u064a \u0648\u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u064a \u0644\u0628\u0628\u062a\u064a\u062f <em>Eumenitin-sfGFP<\/em> \u00a0\u0646\u062c\u0627\u062d\u064b\u0627 \u0643\u0627\u0645\u0644\u0627\u064b \u0641\u064a \u0625\u0646\u062a\u0627\u062c \u0627\u0644\u0646\u0633\u062e\u0629 \u0627\u0644\u0645\u0624\u0634\u0628\u0629 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0646\u0638\u0627\u0645 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0641\u064a <em>Escherichia coli<\/em>. \u00a0\u062a\u0645 \u062a\u0623\u0643\u064a\u062f \u0635\u062d\u0629 \u0627\u0644\u0625\u062f\u062e\u0627\u0644 \u0627\u0644\u062c\u064a\u0646\u064a \u0645\u0646 \u062e\u0644\u0627\u0644 \u062a\u062d\u0644\u064a\u0644 \u062a\u0641\u0627\u0639\u0644 \u0627\u0644\u0628\u0648\u0644\u064a\u0645\u064a\u0631\u0627\u0632 \u0627\u0644\u0645\u062a\u0633\u0644\u0633\u0644 (PCR) \u00a0\u0648\u062a\u0648\u0627\u0641\u0642 \u0627\u0644\u062a\u0633\u0644\u0633\u0644 \u0628\u0646\u0633\u0628\u0629 100% \u0645\u0639 \u0627\u0644\u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u0645\u0635\u0645\u0645\u060c \u0645\u0645\u0627 \u064a\u062f\u0644 \u0639\u0644\u0649 \u062f\u0642\u0629 \u0627\u0644\u062a\u0635\u0645\u064a\u0645 \u0648\u062e\u0644\u0648 \u0627\u0644\u0628\u0646\u064a\u0629 \u0627\u0644\u0628\u0644\u0627\u0632\u0645\u064a\u062f\u064a\u0629 \u0645\u0646 \u0627\u0644\u0637\u0641\u0631\u0627\u062a [14]. \u0648\u064a\u064f\u0639\u062f \u0646\u0638\u0627\u0645 <em>E. coli<\/em> \u0645\u0646 \u0627\u0644\u0623\u0646\u0638\u0645\u0629 \u0627\u0644\u0645\u064f\u0641\u0636\u0644\u0629 \u0641\u064a \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u064a \u0646\u062a\u064a\u062c\u0629 \u0644\u0645\u0639\u062f\u0644 \u0646\u0645\u0648\u0647 \u0627\u0644\u0633\u0631\u064a\u0639\u060c \u0648\u0627\u0646\u062e\u0641\u0627\u0636 \u062a\u0643\u0627\u0644\u064a\u0641 \u0632\u0631\u0627\u0639\u062a\u0647\u060c \u0648\u0633\u0647\u0648\u0644\u0629 \u0627\u0644\u062a\u0644\u0627\u0639\u0628 \u0627\u0644\u062c\u064a\u0646\u064a \u0628\u0647 [17]. \u0639\u064f\u0632\u064a \u0638\u0647\u0648\u0631 \u0627\u0644\u0639\u0635\u0627\u0628\u0629 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u064a\u0629 \u0639\u0646\u062f ~38 \u0643\u064a\u0644\u0648\u062f\u0627\u0644\u062a\u0648\u0646 \u0625\u0644\u0649 \u0627\u0644\u0628\u0646\u064a\u0629 \u0627\u0644\u0645\u0624\u0634\u0628\u0629 \u0627\u0644\u0645\u0635\u0645\u0645\u0629\u060c \u0641\u064a \u062d\u064a\u0646 \u0623\u0646 \u0627\u0644\u0648\u0632\u0646 \u0627\u0644\u062c\u0632\u064a\u0626\u064a \u0627\u0644\u0646\u0638\u0631\u064a \u0644\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0645\u0624\u0634\u0651\u0628 (Eumenitin + sfGFP + His-tag + linker) \u064a\u064f\u0642\u062f\u0651\u064e\u0631 \u0628\u0640 ~31 \u0643\u064a\u0644\u0648\u062f\u0627\u0644\u062a\u0648\u0646 \u0627\u0633\u062a\u0646\u0627\u062f\u064b\u0627 \u0625\u0644\u0649 \u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u0623\u062d\u0645\u0627\u0636 \u0627\u0644\u0623\u0645\u064a\u0646\u064a\u0629 \u0648\u0627\u0644\u062a\u064a \u062a\u062a\u0643\u0648\u0651\u0646 \u0645\u0646 \u0627\u0644\u0628\u0628\u062a\u064a\u062f <em>Eumenitin<\/em> 1645 \u062f\u0627\u0644\u062a\u0648\u0646\u060c \u0645\u0639 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0641\u0644\u0648\u0631\u064a sfGFP 27500 \u062f\u0627\u0644\u062a\u0648\u0646\u060c \u0625\u0636\u0627\u0641\u0629\u064b \u0625\u0644\u0649 \u0648\u0635\u0644\u0629 \u0645\u0631\u0646\u0629 \u0648\u062a\u0633\u0644\u0633\u0644 His-tag.. \u064a\u064f\u0639\u0632\u0649 \u0647\u0630\u0627 \u0627\u0644\u0627\u062e\u062a\u0644\u0627\u0641 \u0628\u064a\u0646 \u0627\u0644\u0648\u0632\u0646 \u0627\u0644\u0645\u062a\u0648\u0642\u0639 \u0648\u0627\u0644\u0645\u0644\u0627\u062d\u0638 \u0625\u0644\u0649 \u0637\u0628\u064a\u0639\u0629 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0645\u0624\u0634\u0651\u0628 \u0648\u0627\u0631\u062a\u0628\u0627\u0637\u0647 \u0628\u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0641\u0644\u0648\u0631\u064a \u0627\u0644\u0623\u062e\u0636\u0631 (sfGFP)\u060c \u0625\u0630 \u064a\u0645\u0643\u0646 \u0623\u0646 \u062a\u0624\u062f\u064a \u0627\u0644\u062a\u063a\u064a\u0631\u0627\u062a \u0641\u064a \u0637\u064a \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0623\u0648 \u0634\u062d\u0646\u062a\u0647 \u0627\u0644\u0643\u0647\u0631\u0628\u064a\u0629 \u0625\u0644\u0649 \u0625\u0628\u0637\u0627\u0621 \u0647\u062c\u0631\u062a\u0647 \u0641\u064a \u0647\u0644\u0627\u0645 SDS-PAGE \u0641\u064a\u0638\u0647\u0631 \u0628\u0648\u0632\u0646 \u0623\u0639\u0644\u0649 \u0645\u0646 \u0627\u0644\u062d\u0633\u0627\u0628\u0627\u062a \u0627\u0644\u0646\u0638\u0631\u064a\u0629. \u0648\u0642\u062f \u0648\u062b\u0651\u0642\u062a \u062f\u0631\u0627\u0633\u0627\u062a \u0633\u0627\u0628\u0642\u0629 \u0647\u0630\u0647 \u0627\u0644\u0638\u0627\u0647\u0631\u0629 \u0639\u0646\u062f \u062f\u0645\u062c \u0628\u0628\u062a\u064a\u062f\u0627\u062a \u0642\u0635\u064a\u0631\u0629 \u0623\u0648 \u0645\u0636\u0627\u062f\u0629 \u0644\u0644\u0645\u064a\u0643\u0631\u0648\u0628\u0627\u062a \u0645\u0639 GFP\u060c \u062d\u064a\u062b \u0628\u0642\u064a\u062a \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0624\u0634\u0651\u0628\u0629 \u0645\u0633\u062a\u0642\u0631\u0629 \u0648\u0641\u0639\u0651\u0627\u0644\u0629 \u062f\u0648\u0646 \u0623\u0646 \u064a\u0624\u062b\u0631 \u0630\u0644\u0643 \u0633\u0644\u0628\u064b\u0627 \u0639\u0644\u0649 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0623\u0648 \u0627\u0644\u0642\u0627\u0628\u0644\u064a\u0629 \u0644\u0644\u0630\u0648\u0628\u0627\u0646 [[18,19 \u062d\u064f\u062f\u062f \u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0645\u0646\u0642\u0649 \u00a0\u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u062e\u062a\u0628\u0627\u0631\u00a0 Bradford138.83 \u0645\u064a\u0643\u0631\u0648\u063a\u0631\u0627\u0645\/\u0645\u0644.<\/p>\n<p>\u062a\u062a\u0641\u0642 \u0647\u0630\u0647 \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0645\u0639 \u062f\u0631\u0627\u0633\u0627\u062a \u0633\u0627\u0628\u0642\u0629 \u0623\u062b\u0628\u062a\u062a \u0625\u0645\u0643\u0627\u0646\u064a\u0629 \u062f\u0645\u062c \u0628\u0628\u062a\u064a\u062f\u0627\u062a \u0641\u0639\u0627\u0644\u0629 \u062d\u064a\u0648\u064a\u064b\u0627 \u0636\u0645\u0646 \u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0645\u0624\u0634\u0651\u0628\u0629 \u062f\u0648\u0646 \u0627\u0644\u062a\u0623\u062b\u064a\u0631 \u0627\u0644\u0633\u0644\u0628\u064a \u0639\u0644\u0649 \u0639\u0645\u0644\u064a\u0629 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\u0639\u062f\u0645 \u0625\u062c\u0631\u0627\u0621 \u062a\u0642\u064a\u064a\u0645\u0627\u062a \u062d\u064a\u0648\u064a\u0629 \u062f\u0627\u062e\u0644 \u0627\u0644\u062c\u0633\u0645 \u0627\u0644\u062d\u064a (in vivo). \u064a\u064f\u0648\u0635\u0649 \u0628\u0645\u0639\u0627\u0644\u062c\u0629 \u0647\u0630\u0647 \u0627\u0644\u062c\u0648\u0627\u0646\u0628 \u0643\u0645\u0627 \u064a\u064f\u0648\u0635\u0649 \u0628\u062a\u0639\u0632\u064a\u0632 \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0639\u064a\u0627\u0631\u0627\u062a \u0625\u0636\u0627\u0641\u064a\u0629 \u0645\u062b\u0644 \u0627\u062e\u062a\u0628\u0627\u0631 \u0628\u0631\u0648\u062a\u064a\u0646 GFP \u0644\u0648\u062d\u062f\u0647 \u0644\u0644\u062a\u0623\u0643\u062f \u0645\u0646 \u062e\u0644\u0648\u0647 \u0645\u0646 \u0627\u0644\u0646\u0634\u0627\u0637 \u0627\u0644\u0645\u0636\u0627\u062f \u0644\u0644\u062c\u0631\u0627\u062b\u064a\u0645\u060c \u0648\u0627\u062e\u062a\u0628\u0627\u0631 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u0645\u0624\u0634\u0651\u064e\u0628 \u0645\u0639 \u0643\u0631\u064a\u0627\u062a \u062f\u0645 \u0628\u0634\u0631\u064a\u0629 \u0641\u064a \u0645\u0633\u062a\u0646\u0628\u062a \u062e\u0644\u0648\u064a \u0644\u0625\u062b\u0628\u0627\u062a \u0639\u062f\u0645 \u0633\u0645\u064a\u062a\u0647. \u0643\u0645\u0627 \u064a\u064f\u0633\u062a\u062d\u0633\u0646 \u0641\u0635\u0644 \u0627\u0644\u0628\u0646\u064a\u0629 \u0627\u0644\u0648\u0638\u064a\u0641\u064a\u0629 \u0644\u0644\u0628\u0628\u062a\u064a\u062f \u0639\u0646 \u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u062a\u062a\u0628\u0639 GFP \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0648\u0635\u0644\u0629 \u0625\u0646\u0632\u064a\u0645\u064a\u0629 \u0642\u0627\u0628\u0644\u0629 \u0644\u0644\u0642\u0637\u0639 \u0645\u062b\u0644 TEV linker\u060c \u0628\u0645\u0627 \u064a\u0633\u0645\u062d \u0628\u062a\u0642\u064a\u064a\u0645 \u0627\u0644\u0646\u0634\u0627\u0637 \u0627\u0644\u062d\u064a\u0648\u064a \u0644\u0644\u0628\u0628\u062a\u064a\u062f \u0628\u0635\u064a\u063a\u062a\u0647 \u0627\u0644\u0646\u0642\u064a\u0629 \u0648\u0627\u0644\u0645\u0633\u062a\u0642\u0644\u0629.<\/p>\n<p>\u0628\u0646\u0627\u0621\u064b \u0639\u0644\u0649 \u0645\u0627 \u0633\u0628\u0642\u060c \u064a\u064f\u0639\u062f \u0627\u0644\u062a\u0635\u0645\u064a\u0645 \u0627\u0644\u0645\u0624\u0634\u0651\u0628 \u00a0<em>Eumenitin-sfGFP<\/em> \u0646\u0645\u0648\u0630\u062c\u064b\u0627 \u0648\u0627\u0639\u062f\u064b\u0627 \u0644\u0625\u0646\u062a\u0627\u062c \u0628\u0628\u062a\u064a\u062f\u0627\u062a \u0641\u0639\u0627\u0644\u0629 \u062d\u064a\u0648\u064a\u064b\u0627 \u0628\u0637\u0631\u064a\u0642\u0629 \u0645\u0633\u062a\u0642\u0631\u0629 \u0648\u0642\u0627\u0628\u0644\u0629 \u0644\u0644\u062a\u062a\u0628\u0639\u060c \u0645\u0639 \u0627\u0644\u062d\u0641\u0627\u0638 \u0639\u0644\u0649 \u0646\u0634\u0627\u0637\u0647\u0627 \u0627\u0644\u0645\u0636\u0627\u062f \u0644\u0644\u0645\u064a\u0643\u0631\u0648\u0628\u0627\u062a. \u0648\u062a\u0634\u064a\u0631 \u0627\u0644\u0623\u062f\u0628\u064a\u0627\u062a \u0625\u0644\u0649 \u0623\u0646 <em>Eumenitin<\/em> \u00a0\u064a\u062a\u0645\u062a\u0639 \u0628\u0645\u0644\u0641 \u0623\u0645\u0627\u0646 \u062d\u064a\u0648\u064a \u062c\u064a\u062f\u060c \u0645\u0645\u0627 \u064a\u0639\u0632\u0632 \u0645\u0646 \u0625\u0645\u0643\u0627\u0646\u064a\u0627\u062a\u0647 \u0643\u0645\u0631\u0634\u062d \u0639\u0644\u0627\u062c\u064a \u0645\u062d\u062a\u0645\u0644 \u0641\u064a \u0638\u0644 \u062a\u0632\u0627\u064a\u062f \u0645\u0642\u0627\u0648\u0645\u0629 \u0627\u0644\u0645\u0636\u0627\u062f\u0627\u062a \u0627\u0644\u062d\u064a\u0648\u064a\u0629.<\/p>\n<p>\u062a\u064f\u0638\u0647\u0631 \u0647\u0630\u0647 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0623\u0646 \u062f\u0645\u062c \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0627\u0644\u062d\u064a\u0648\u064a <em>Eumenitin<\/em> \u00a0\u0628\u0628\u0631\u0648\u062a\u064a\u0646 <em>sfGFP<\/em> \u00a0\u0644\u0645 \u064a\u0624\u062b\u0631 \u0639\u0644\u0649 \u0646\u0634\u0627\u0637\u0647 \u0627\u0644\u0645\u0636\u0627\u062f \u0644\u0644\u0645\u064a\u0643\u0631\u0648\u0628\u0627\u062a\u060c \u0645\u0645\u0627 \u064a\u062f\u0639\u0645 \u062c\u062f\u0648\u0649 \u0647\u0630\u0627 \u0627\u0644\u062a\u0635\u0645\u064a\u0645 \u0644\u062a\u0637\u0648\u064a\u0631 \u0623\u0646\u0638\u0645\u0629 \u062a\u062a\u0628\u0639 \u0628\u0635\u0631\u064a\u0629 \u0623\u0648 \u062f\u0645\u062c\u0647 \u0641\u064a \u0635\u064a\u063a \u062f\u0648\u0627\u0626\u064a\u0629 \u0645\u0633\u062a\u0642\u0628\u0644\u064a\u0629. \u0643\u0645\u0627 \u064a\u064f\u0648\u0635\u0649 \u0641\u064a \u0627\u0644\u0623\u0628\u062d\u0627\u062b \u0627\u0644\u0644\u0627\u062d\u0642\u0629 \u0628\u0641\u0635\u0644 \u0627\u0644\u0628\u0628\u062a\u064a\u062f \u0639\u0646 \u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u062a\u062a\u0628\u0639 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0648\u0635\u0644\u0629 \u0625\u0646\u0632\u064a\u0645\u064a\u0629 \u0642\u0627\u0628\u0644\u0629 \u0644\u0644\u0642\u0637\u0639 \u0645\u062b\u0644 <em>TEV linker<\/em> \u0644\u0644\u062d\u0635\u0648\u0644 \u0639\u0644\u0649 \u0646\u0633\u062e\u0629 \u0646\u0642\u064a\u0629 \u0644\u0644\u062a\u0642\u064a\u064a\u0645 \u0627\u0644\u062d\u064a\u0648\u064a \u0627\u0644\u0645\u0633\u062a\u0642\u0644.<\/p>\n<p>\u0623\u0643\u062f \u0646\u0638\u0627\u0645 \u0627\u0644\u062a\u0639\u0628\u064a\u0631 \u0627\u0644\u0645\u0639\u062a\u0645\u062f \u0641\u064a <em>E. coli<\/em> \u00a0\u0643\u0641\u0627\u0621\u062a\u0647 \u0641\u064a \u0625\u0646\u062a\u0627\u062c \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0645\u0624\u0634\u0628 \u0628\u0643\u0645\u064a\u0627\u062a \u062c\u064a\u062f\u0629 \u0648\u0646\u0642\u0627\u0621 \u0639\u0627\u0644\u064d \u0648\u0628\u062a\u0643\u0644\u0641\u0629 \u0645\u0646\u062e\u0641\u0636\u0629\u060c \u0645\u0645\u0627 \u064a\u062c\u0639\u0644\u0647 \u0645\u0646\u0635\u0629 \u0645\u0646\u0627\u0633\u0628\u0629 \u0644\u0625\u0646\u062a\u0627\u062c \u0627\u0644\u0628\u0628\u062a\u064a\u062f\u0627\u062a \u0627\u0644\u062d\u064a\u0648\u064a\u0629 [26]. \u062a\u0648\u0635\u064a \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0628\u0625\u062c\u0631\u0627\u0621 \u0627\u062e\u062a\u0628\u0627\u0631\u0627\u062a \u0643\u0645\u064a\u0629 \u0623\u0643\u062b\u0631 \u062f\u0642\u0629 (\u0645\u062b\u0644 MIC \u0648MBC) \u0648\u062a\u0648\u0633\u064a\u0639 \u0627\u0644\u062a\u0642\u064a\u064a\u0645 \u0644\u064a\u0634\u0645\u0644 \u062f\u0631\u0627\u0633\u0627\u062a \u062f\u0627\u062e\u0644 \u0627\u0644\u062c\u0633\u0645 \u0627\u0644\u062d\u064a (in vivo)\u060c \u0628\u0647\u062f\u0641 \u0627\u0633\u062a\u0643\u0645\u0627\u0644 \u0627\u0644\u062a\u0648\u0635\u064a\u0641 \u0627\u0644\u062d\u064a\u0648\u064a \u0648\u0627\u0644\u062a\u062d\u0642\u0642 \u0645\u0646 \u0625\u0645\u0643\u0627\u0646\u064a\u0627\u062a \u00a0<em>Eumenitin<\/em> \u00a0\u0643\u0645\u0636\u0627\u062f \u0645\u064a\u0643\u0631\u0648\u0628\u064a \u0648\u0627\u0639\u062f \u0641\u064a \u0645\u0648\u0627\u062c\u0647\u0629 \u0645\u0642\u0627\u0648\u0645\u0629 \u0627\u0644\u0645\u0636\u0627\u062f\u0627\u062a \u0627\u0644\u062d\u064a\u0648\u064a\u0629.<\/p>\n<p><strong>\u0642\u0627\u0626\u0645\u0629 \u0627\u0644\u0627\u062e\u062a\u0635\u0627\u0631\u0627\u062a <\/strong><strong>List of abbreviations<\/strong><\/p>\n<table style=\"width: 73.1036%;\">\n<tbody>\n<tr>\n<td style=\"width: 23.1464%; text-align: center;\" width=\"192\"><strong>\u0627\u0644\u0627\u062e\u062a\u0635\u0627\u0631<\/strong><\/td>\n<td style=\"width: 42.5102%; text-align: center;\" width=\"192\"><strong>\u0627\u0644\u0644\u063a\u0629 \u0627\u0644\u0639\u0631\u0628\u064a\u0629<\/strong><\/td>\n<td style=\"width: 54.8822%; text-align: center;\" width=\"192\"><strong>\u0627\u0644\u0644\u063a\u0629 \u0627\u0644\u0625\u0646\u0643\u0644\u064a\u0632\u064a\u0629<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 23.1464%; text-align: center;\" width=\"192\">AMP<\/td>\n<td style=\"width: 42.5102%; text-align: center;\" width=\"192\">\u0628\u0628\u062a\u064a\u062f \u0645\u0636\u0627\u062f \u0644\u0644\u0645\u064a\u0643\u0631\u0648\u0628\u0627\u062a<\/td>\n<td style=\"width: 54.8822%; text-align: center;\" width=\"192\">Antimicrobial Peptide<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 23.1464%; text-align: center;\" width=\"192\">BSA<\/td>\n<td style=\"width: 42.5102%; text-align: center;\" width=\"192\">\u0623\u0644\u0628\u0648\u0645\u064a\u0646 \u0645\u0635\u0644 \u0627\u0644\u0628\u0642\u0631<\/td>\n<td style=\"width: 54.8822%; text-align: center;\" width=\"192\">Bovine Serum Albumin<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 23.1464%; text-align: center;\" width=\"192\">CFU<\/td>\n<td style=\"width: 42.5102%; text-align: center;\" width=\"192\">\u0648\u062d\u062f\u0629 \u062a\u0634\u0643\u064a\u0644 \u0645\u0633\u062a\u0639\u0645\u0631\u0629<\/td>\n<td style=\"width: 54.8822%; text-align: center;\" width=\"192\">Colony Forming Unit<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 23.1464%; text-align: center;\" width=\"192\">CLSI<\/td>\n<td style=\"width: 42.5102%; text-align: center;\" width=\"192\">\u0645\u0639\u0647\u062f \u0627\u0644\u0645\u0639\u0627\u064a\u064a\u0631 \u0627\u0644\u0633\u0631\u064a\u0631\u064a\u0629 \u0648\u0627\u0644\u0645\u062e\u0628\u0631\u064a\u0629<\/td>\n<td style=\"width: 54.8822%; text-align: center;\" width=\"192\">Clinical and Laboratory Standards Institute<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 23.1464%; text-align: center;\" width=\"192\">DNA<\/td>\n<td style=\"width: 42.5102%; text-align: center;\" width=\"192\">\u0627\u0644\u062d\u0645\u0636 \u0627\u0644\u0646\u0648\u0648\u064a \u0627\u0644\u0631\u064a\u0628\u0648\u0632\u064a \u0645\u0646\u0642\u0648\u0635 \u0627\u0644\u0623\u0643\u0633\u062c\u064a\u0646<\/td>\n<td style=\"width: 54.8822%; text-align: center;\" width=\"192\">Deoxyribonucleic Acid<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 23.1464%; text-align: center;\" width=\"192\">E. coli<\/td>\n<td style=\"width: 42.5102%; text-align: center;\" width=\"192\">\u0627\u0644\u0625\u0634\u0631\u064a\u0643\u064a\u0629 \u0627\u0644\u0642\u0648\u0644\u0648\u0646\u064a\u0629<\/td>\n<td style=\"width: 54.8822%; text-align: center;\" width=\"192\">Escherichia coli<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 23.1464%; text-align: center;\" width=\"192\">GFP<\/td>\n<td style=\"width: 42.5102%; text-align: center;\" width=\"192\">\u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0641\u0644\u0648\u0631\u064a \u0627\u0644\u0623\u062e\u0636\u0631<\/td>\n<td style=\"width: 54.8822%; text-align: center;\" width=\"192\">Green Fluorescent Protein<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 23.1464%; text-align: center;\" width=\"192\">His-tag<\/td>\n<td style=\"width: 42.5102%; text-align: center;\" width=\"192\">\u0648\u0633\u0645\u0629 \u0627\u0644\u0647\u064a\u0633\u062a\u064a\u062f\u064a\u0646<\/td>\n<td style=\"width: 54.8822%; text-align: center;\" width=\"192\">Histidine Tag<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 23.1464%; text-align: center;\" width=\"192\">IMAC<\/td>\n<td style=\"width: 42.5102%; text-align: center;\" width=\"192\">\u0643\u0631\u0648\u0645\u0627\u062a\u0648\u063a\u0631\u0627\u0641\u064a\u0627 \u0627\u0644\u0625\u0644\u0641\u0629 \u0627\u0644\u0645\u0639\u062f\u0646\u064a\u0629<\/td>\n<td style=\"width: 54.8822%; text-align: center;\" width=\"192\">Immobilized Metal Affinity Chromatography<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 23.1464%; text-align: center;\" width=\"192\">IPTG<\/td>\n<td style=\"width: 42.5102%; text-align: center;\" width=\"192\">\u0623\u064a\u0632\u0648\u0628\u0631\u0648\u0628\u064a\u0644 \u062b\u064a\u0648-\u03b2-D-\u062c\u0627\u0644\u0627\u0643\u062a\u0648\u0632\u064a\u062f<\/td>\n<td style=\"width: 54.8822%; text-align: center;\" width=\"192\">Isopropyl \u03b2-D-1-thiogalactopyranoside<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 23.1464%; text-align: center;\" width=\"192\">LB<\/td>\n<td style=\"width: 42.5102%; text-align: center;\" width=\"192\">\u0648\u0633\u0637 \u0644\u0648\u0631\u064a\u0627-\u0628\u064a\u0631\u062a\u0627\u0646\u064a<\/td>\n<td style=\"width: 54.8822%; text-align: center;\" width=\"192\">Luria-Bertani medium<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 23.1464%; text-align: center;\" width=\"192\">MBC<\/td>\n<td style=\"width: 42.5102%; text-align: center;\" width=\"192\">\u0627\u0644\u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0642\u0627\u062a\u0644 \u0627\u0644\u0623\u062f\u0646\u0649<\/td>\n<td style=\"width: 54.8822%; text-align: center;\" width=\"192\">Minimum Bactericidal Concentration<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 23.1464%; text-align: center;\" width=\"192\">MIC<\/td>\n<td style=\"width: 42.5102%; text-align: center;\" width=\"192\">\u0627\u0644\u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0645\u062b\u0628\u0637 \u0627\u0644\u0623\u062f\u0646\u0649<\/td>\n<td style=\"width: 54.8822%; text-align: center;\" width=\"192\">Minimum Inhibitory Concentration<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 23.1464%; text-align: center;\" width=\"192\">Ni-NTA<\/td>\n<td style=\"width: 42.5102%; text-align: center;\" width=\"192\">\u0631\u0627\u062a\u0646\u062c \u0646\u064a\u062a\u0631\u064a\u0644\u0648\u062a\u0631\u064a \u0623\u0633\u064a\u062a\u064a\u0643 \u0627\u0644\u0646\u064a\u0643\u0644<\/td>\n<td style=\"width: 54.8822%; text-align: center;\" width=\"192\">Nickel-Nitrilotriacetic Acid<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 23.1464%; text-align: center;\" width=\"192\">PCR<\/td>\n<td style=\"width: 42.5102%; text-align: center;\" width=\"192\">\u062a\u0641\u0627\u0639\u0644 \u0627\u0644\u0628\u0644\u0645\u0631\u0629 \u0627\u0644\u0645\u062a\u0633\u0644\u0633\u0644<\/td>\n<td style=\"width: 54.8822%; text-align: center;\" width=\"192\">Polymerase Chain Reaction<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 23.1464%; text-align: center;\" width=\"192\">SDS-PAGE<\/td>\n<td style=\"width: 42.5102%; text-align: center;\" width=\"192\">\u0627\u0644\u0631\u062d\u0644\u0627\u0646 \u0627\u0644\u0643\u0647\u0631\u0628\u0627\u0626\u064a \u0644\u0644\u0647\u0644\u0627\u0645 \u0628\u0648\u0644\u064a \u0623\u0643\u0631\u064a\u0644\u0627\u0645\u064a\u062f \u0645\u0639 SDS<\/td>\n<td style=\"width: 54.8822%; text-align: center;\" width=\"192\">Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 23.1464%; text-align: center;\" width=\"192\">sfGFP<\/td>\n<td style=\"width: 42.5102%; text-align: center;\" width=\"192\">\u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0641\u0644\u0648\u0631\u064a \u0627\u0644\u0623\u062e\u0636\u0631 \u0627\u0644\u0641\u0627\u0626\u0642<\/td>\n<td style=\"width: 54.8822%; text-align: center;\" width=\"192\">Superfolder Green Fluorescent Protein<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 23.1464%; text-align: center;\" width=\"192\">SPSS<\/td>\n<td style=\"width: 42.5102%; text-align: center;\" width=\"192\">\u0627\u0644\u062d\u0632\u0645\u0629 \u0627\u0644\u0625\u062d\u0635\u0627\u0626\u064a\u0629 \u0644\u0644\u0639\u0644\u0648\u0645 \u0627\u0644\u0627\u062c\u062a\u0645\u0627\u0639\u064a\u0629<\/td>\n<td style=\"width: 54.8822%; text-align: center;\" width=\"192\">Statistical Package for the Social Sciences<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 23.1464%; text-align: center;\" width=\"192\">WHO<\/td>\n<td style=\"width: 42.5102%; text-align: center;\" width=\"192\">\u0645\u0646\u0638\u0645\u0629 \u0627\u0644\u0635\u062d\u0629 \u0627\u0644\u0639\u0627\u0644\u0645\u064a\u0629<\/td>\n<td style=\"width: 54.8822%; text-align: center;\" width=\"192\">World Health Organization<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>\u062a\u0636\u0627\u0631\u0628 \u0627\u0644\u0645\u0635\u0627\u0644\u062d <\/strong><strong>Conflict of interests<\/strong><\/p>\n<p>\u064a\u064f\u0642\u0631\u0651 \u062c\u0645\u064a\u0639 \u0627\u0644\u0628\u0627\u062d\u062b\u064a\u0646 \u0627\u0644\u0645\u0634\u0627\u0631\u0643\u064a\u0646 \u0641\u064a \u0647\u0630\u0627 \u0627\u0644\u0628\u062d\u062b \u0628\u0639\u062f\u0645 \u0648\u062c\u0648\u062f \u062a\u0636\u0627\u0631\u0628 \u0641\u064a \u0627\u0644\u0645\u0635\u0627\u0644\u062d \u0641\u064a\u0645\u0627 \u0628\u064a\u0646\u0647\u0645.<\/p>\n<p><strong>Funding \u0627\u0644\u062a\u0645\u0648\u064a\u0644<\/strong><\/p>\n<p>\u0645\u064f\u0648\u0651\u0650\u0644 \u0647\u0630\u0627 \u0627\u0644\u0628\u062d\u062b \u062c\u0632\u0626\u064a\u064b\u0627 \u0645\u0646 \u0642\u0628\u0644 \u062c\u0627\u0645\u0639\u0629 \u062f\u0645\u0634\u0642 \u0648\u062a\u062d\u0645\u0651\u0644\u062a \u0627\u0644\u0628\u0627\u062d\u062b\u0629 \u062c\u0645\u064a\u0639 \u0627\u0644\u062a\u0643\u0627\u0644\u064a\u0641 \u0627\u0644\u0623\u062e\u0631\u0649.<\/p>\n<p><strong>\u0627\u0644\u0634\u0643\u0631 \u0648\u0627\u0644\u062a\u0642\u062f\u064a\u0631<\/strong> <strong>Acknowledgements <\/strong><\/p>\n<p>\u0627\u062a\u0642\u062f\u0645 \u0628\u062e\u0627\u0644\u0635 \u0627\u0644\u0634\u0643\u0631 \u0648\u0627\u0644\u0627\u0645\u062a\u0646\u0627\u0646 \u0625\u0644\u0649 \u0647\u064a\u0626\u0629 \u0627\u0644\u0637\u0627\u0642\u0629 \u0627\u0644\u0630\u0631\u064a\u0629 \u0627\u0644\u0633\u0648\u0631\u064a\u0629 \u2013 \u0642\u0633\u0645 \u0627\u0644\u0628\u064a\u0648\u0644\u0648\u062c\u064a\u0627 \u0627\u0644\u062c\u0632\u064a\u0626\u064a\u0629 \u0648\u0627\u0644\u062a\u0642\u0627\u0646\u0629 \u0627\u0644\u062d\u064a\u0648\u064a\u0629\u060c \u0648\u0639\u0644\u0649 \u0631\u0623\u0633\u0647\u0627 \u0627\u0644\u062f\u0643\u062a\u0648\u0631 \u0623\u0646\u0637\u0648\u0646\u064a\u0648\u0633 \u0627\u0644\u062f\u0627\u0648\u062f\u060c \u0631\u0626\u064a\u0633 \u0627\u0644\u0642\u0633\u0645\u060c \u0644\u0645\u0627 \u0642\u062f\u0645\u0648\u0647 \u0645\u0646 \u062f\u0639\u0645 \u0639\u0644\u0645\u064a \u0648\u062a\u0642\u0646\u064a\u060c \u0648\u062a\u0648\u0641\u064a\u0631 \u0627\u0644\u0625\u0645\u0643\u0627\u0646\u0627\u062a \u0648\u0627\u0644\u062a\u0633\u0647\u064a\u0644\u0627\u062a \u0627\u0644\u062a\u064a \u0633\u0627\u0647\u0645\u062a \u0641\u064a \u062a\u0646\u0641\u064a\u0630 \u0647\u0630\u0627 \u0627\u0644\u0628\u062d\u062b. \u0648\u0623\u0639\u0631\u0628 \u0639\u0646 \u0628\u0627\u0644\u063a \u0627\u0644\u062a\u0642\u062f\u064a\u0631 \u0648\u0627\u0644\u0627\u0645\u062a\u0646\u0627\u0646 \u0644\u0640\u0644\u062f\u0643\u062a\u0648\u0631 \u0639\u0628\u062f \u0627\u0644\u0642\u0627\u062f\u0631 \u0639\u0628\u0627\u062f\u064a \u0639\u0644\u0649 \u062a\u0648\u062c\u064a\u0647\u0627\u062a\u0647 \u0627\u0644\u0639\u0644\u0645\u064a\u0629 \u0627\u0644\u0642\u064a\u0651\u0645\u0629\u060c \u0648\u0645\u062a\u0627\u0628\u0639\u062a\u0647 \u0627\u0644\u062f\u0642\u064a\u0642\u0629 \u0644\u0645\u0631\u0627\u062d\u0644 \u0627\u0644\u0639\u0645\u0644\u060c \u0648\u0625\u0644\u0649 \u0627\u0644\u062f\u0643\u062a\u0648\u0631 \u0623\u064a\u0645\u0646 \u0627\u0644\u0645\u0631\u064a\u0631\u064a \u0639\u0644\u0649 \u0645\u0627 \u0642\u062f\u0645\u0647 \u0645\u0646 \u062f\u0639\u0645 \u0648\u0625\u062a\u0627\u062d\u0629 \u0627\u0644\u0641\u0631\u0635\u0629 \u0644\u0644\u0627\u0646\u062e\u0631\u0627\u0637 \u0641\u064a \u0647\u0630\u0627 \u0627\u0644\u0645\u062c\u0627\u0644 \u0627\u0644\u0628\u062d\u062b\u064a.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u0627\u0644\u0645\u0642\u062f\u0645\u0629 Introduction \u062a\u064f\u0639\u062f \u0645\u0642\u0627\u0648\u0645\u0629 \u0627\u0644\u062c\u0631\u0627\u062b\u064a\u0645 \u0644\u0644\u0645\u0636\u0627\u062f\u0627\u062a \u0627\u0644\u062d\u064a\u0648\u064a\u0629 \u0645\u0646 \u0623\u062e\u0637\u0631 \u0627\u0644\u062a\u062d\u062f\u064a\u0627\u062a \u0627\u0644\u0635\u062d\u064a\u0629 \u0627\u0644\u0639\u0627\u0644\u0645\u064a\u0629 \u0641\u064a \u0627\u0644\u0642\u0631\u0646 \u0627\u0644\u062d\u0627\u062f\u064a \u0648\u0627\u0644\u0639\u0634\u0631\u064a\u0646\u060c \u0648\u0635\u0641\u062a\u0647\u0627 \u0645\u0646\u0638\u0645\u0629 \u0627\u0644\u0635\u062d\u0629 \u0627\u0644\u0639\u0627\u0644\u0645\u064a\u0629 \u0628\u0623\u0646\u0647\u0627 \u062a\u0647\u062f\u064a\u062f&#8230; <br \/><a href=\"https:\/\/pharmjournal.asscph.com\/?p=2129\">\u0627\u0642\u0631\u0623 \u0627\u0644\u0645\u0632\u064a\u062f<\/a><\/p>\n","protected":false},"author":2,"featured_media":2140,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[136],"tags":[],"class_list":["post-2129","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-136","issue-158","volume-9","year-159"],"acf":[],"_links":{"self":[{"href":"https:\/\/pharmjournal.asscph.com\/index.php?rest_route=\/wp\/v2\/posts\/2129","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pharmjournal.asscph.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pharmjournal.asscph.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pharmjournal.asscph.com\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/pharmjournal.asscph.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2129"}],"version-history":[{"count":5,"href":"https:\/\/pharmjournal.asscph.com\/index.php?rest_route=\/wp\/v2\/posts\/2129\/revisions"}],"predecessor-version":[{"id":2203,"href":"https:\/\/pharmjournal.asscph.com\/index.php?rest_route=\/wp\/v2\/posts\/2129\/revisions\/2203"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pharmjournal.asscph.com\/index.php?rest_route=\/wp\/v2\/media\/2140"}],"wp:attachment":[{"href":"https:\/\/pharmjournal.asscph.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2129"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pharmjournal.asscph.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2129"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pharmjournal.asscph.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2129"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}