{"id":87,"date":"2023-01-01T08:48:45","date_gmt":"2023-01-01T08:48:45","guid":{"rendered":"https:\/\/pharmjournal.asscph.com\/?p=87"},"modified":"2024-07-21T17:34:03","modified_gmt":"2024-07-21T17:34:03","slug":"%d8%aa%d8%b9%d9%8a%d9%8a%d9%86-%d8%ae%d8%b5%d8%a7%d8%a6%d8%b5-%d8%ab%d8%a8%d8%a7%d8%aa-%d8%a7%d9%84%d8%ba%d9%84%d9%88%d8%a8%d9%88%d9%84%d9%8a%d9%86%d8%a7%d8%aa-%d8%a7%d9%84%d9%85%d9%86%d8%a7%d8%b9","status":"publish","type":"post","link":"https:\/\/pharmjournal.asscph.com\/?p=87","title":{"rendered":"\u062a\u0639\u064a\u064a\u0646 \u062e\u0635\u0627\u0626\u0635 \u062b\u0628\u0627\u062a \u0627\u0644\u063a\u0644\u0648\u0628\u0648\u0644\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 IgGs \u0644\u062c\u0645\u0644 \u0645\u064f\u0645\u064e\u0646\u0651\u064e\u0639 \u062a\u062c\u0627\u0647 \u0633\u0645 \u0627\u0644\u0639\u0642\u0631\u0628 \u0648\u062a\u0646\u0642\u064a\u062a\u0647\u0627 \u0648\u0643\u0634\u0641\u0647\u0627"},"content":{"rendered":"<p 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\u0628\u0634\u0643\u0644\u0647\u0627 \u0627\u0644\u0645\u0637\u0648\u064a \u0627\u0644\u0641\u0639\u0627\u0644 \u0628\u0639\u062f \u0627\u0644\u062a\u062e\u0644\u0635 \u0645\u0646 \u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0635\u0644 \u0627\u0644\u0623\u062e\u0631\u0649\u060c \u0628\u0645\u0627 \u064a\u062e\u062f\u0645 \u0623\u0647\u062f\u0627\u0641 \u0627\u0644\u0635\u064a\u0627\u063a\u0629 \u0627\u0644\u0635\u064a\u062f\u0644\u0627\u0646\u064a\u0629\u060c \u0648\u0645\u0646 \u062b\u0645 \u0641\u0635\u0644 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u062b\u0642\u064a\u0644\u0629 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 \u0639\u0646 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062a\u0642\u0644\u064a\u062f\u064a\u0629 \u0648\u0645\u0642\u0627\u0631\u0646\u0629 \u062b\u0628\u0627\u062a \u0627\u0644\u0623\u0646\u0645\u0627\u0637 \u0627\u0644\u0645\u062e\u062a\u0644\u0641\u0629 \u0645\u0646 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u062a\u062c\u0627\u0647 \u0634\u0631\u0648\u0637 \u0627\u0644\u062a\u062e\u0631\u064a\u0628 \u0627\u0644\u0642\u0633\u0631\u064a.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\"><strong>\u0627\u0644\u0645\u0648\u0627\u062f \u0648\u0627\u0644\u0637\u0651\u0631\u0627\u0626\u0642 <\/strong><strong>\u00a0Materials and Methods<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\"><strong>\u0633\u0645 \u0627\u0644\u0639\u0642\u0631\u0628<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\">\u0627\u0633\u062a\u062e\u062f\u0645 \u0633\u0645 \u0627\u0644\u0639\u0642\u0631\u0628 \u0627\u0644\u0645\u062d\u0636\u0631 \u0628\u0627\u0644\u0637\u0631\u064a\u0642\u0629 \u0627\u0644\u0643\u064a\u0645\u064a\u0627\u0626\u064a\u0629\u060c \u0625\u0630 \u062a\u0637\u0628\u0642 \u0633\u0644\u0633\u0644\u0629 \u0645\u0646 \u0627\u0644\u062a\u064a\u0627\u0631\u0627\u062a \u0627\u0644\u0643\u0647\u0631\u0628\u0627\u0626\u064a\u0629 \u0644\u0635\u062f\u0645 \u0627\u0644\u0639\u0642\u0631\u0628 \u0648\u0625\u062e\u0631\u0627\u062c \u0627\u0644\u0633\u0645. \u064a\u063a\u0645\u0631 \u062c\u0633\u0645 \u0627\u0644\u0639\u0642\u0631\u0628 \u0641\u064a \u0645\u062d\u0644\u0648\u0644 \u0645\u0644\u062d\u064a \u0645\u0646 \u0623\u062c\u0644 \u062a\u0648\u0635\u064a\u0644 \u0643\u0647\u0631\u0628\u0627\u0626\u064a \u0623\u0641\u0636\u0644 \u0648\u0635\u062f\u0645\u0647 \u0628\u0627\u0644\u0642\u0637\u0628\u060c \u0648\u0630\u0644\u0643 \u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u0628\u0637\u0627\u0631\u064a\u0629 \u0628\u0633\u064a\u0637\u0629 12 \u0641\u0648\u0644\u062a [7, 8].<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\"><strong>\u0627\u062e\u062a\u0628\u0627\u0631 \u062a\u0631\u0633\u064a\u0628 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0645\u0646 \u0627\u0644\u0645\u0635\u0644 \u0627\u0644\u062f\u0645\u0648\u064a \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0623\u0645\u062b\u0644 \u0645\u0646 \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645:<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\">\u064a\u0632\u062f\u0627\u062f \u0630\u0648\u0628\u0627\u0646 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0628\u0634\u0643\u0644\u0647 \u0627\u0644\u0643\u0631\u0648\u064a \u0639\u0646\u062f \u0625\u0636\u0627\u0641\u0629 \u0645\u0644\u062d \u0628\u062a\u0631\u0627\u0643\u064a\u0632 \u0623\u0642\u0644 \u0645\u0646 0.15 M\u060c \u0648\u062a\u0633\u0645\u0649 \u0647\u0630\u0647 \u0627\u0644\u0639\u0645\u0644\u064a\u0629 \u0628\u0627\u0644\u062a\u0645\u0644\u064a\u062d Salting-in\u060c \u0641\u064a \u062d\u064a\u0646 \u0623\u0646 \u0627\u0644\u062a\u0631\u0627\u0643\u064a\u0632 \u0627\u0644\u0623\u0639\u0644\u0649 \u0645\u0646 \u0627\u0644\u0623\u0645\u0644\u0627\u062d \u062a\u0624\u062f\u064a \u0625\u0644\u0649 \u062a\u0646\u0627\u0642\u0635 \u0630\u0648\u0628\u0627\u0646 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0628\u0639\u0645\u0644\u064a\u0629 \u062a\u0633\u0645\u0649 \u0627\u0644\u062a\u0631\u0633\u064a\u0628 \u0628\u0627\u0644\u062a\u0645\u0644\u064a\u062d Salting-out \u0648\u062a\u0624\u062f\u064a \u0625\u0644\u0649 \u062a\u0631\u0633\u0628\u0647 [9]. \u062a\u062d\u0641\u0632 \u0627\u0644\u0623\u0645\u0644\u0627\u062d \u0627\u0644\u062a\u064a \u0631\u0633\u0628\u062a \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u062b\u0628\u0627\u062a\u0647 \u0645\u0646 \u0627\u0644\u0646\u0627\u062d\u064a\u0629 \u0627\u0644\u0634\u0643\u0644\u064a\u0629\u060c \u0639\u0644\u0649 \u0639\u0643\u0633 \u0627\u0644\u0623\u0645\u0644\u0627\u062d \u0627\u0644\u062a\u064a \u0623\u0633\u0647\u0645\u062a \u0641\u064a \u0632\u064a\u0627\u062f\u0629 \u0630\u0648\u0628\u0627\u0646\u0647 \u0627\u0644\u0630\u064a \u064a\u0643\u0648\u0646 \u0639\u0627\u062f\u0629 \u0645\u0645\u0633\u062e\u0627\u064b Denaturants \u0644\u0644\u0628\u0631\u0648\u062a\u064a\u0646 [10]. <\/span><span style=\"font-family: 'times new roman', times, serif;\">\u0631\u0633\u0628\u062a \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062a\u0631\u0627\u0643\u064a\u0632 \u0645\u062a\u0632\u0627\u064a\u062f\u0629 \u0645\u0646 \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u062a\u062d\u062a \u062e\u064a\u0645\u0629 \u0623\u0645\u0627\u0646 \u062d\u064a\u0648\u064a \u0646\u0638\u064a\u0641\u0629 \u0648\u0645\u0639\u0642\u0645\u0629\u060c \u0625\u0630 \u0628\u062f\u0626 \u0641\u064a \u0643\u0644 \u062e\u0637\u0648\u0629 \u0628\u062d\u062c\u0645 10 \u0645\u0644 \u0636\u0645\u0646 \u0623\u0646\u0628\u0648\u0628 50 \u0645\u0644 \u0648\u064a\u0636\u0627\u0641 \u0645\u0644\u062d \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0634\u064a\u0626\u0627\u064b \u0641\u0634\u064a\u0626\u0627\u064b \u0645\u0639 \u0627\u0644\u062a\u062d\u0631\u064a\u0643\u060c \u062d\u0633\u0628\u062a \u0643\u0645\u064a\u0629 \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0627\u0644\u0648\u0627\u062c\u0628 \u0625\u0636\u0627\u0641\u062a\u0647\u0627 \u0644\u0644\u062d\u0635\u0648\u0644 \u0639\u0644\u0649 \u062f\u0631\u062c\u0629 \u0627\u0644\u0625\u0634\u0628\u0627\u0639 \u0627\u0644\u0645\u0637\u0644\u0648\u0628\u0629 \u0628\u0627\u0644\u0627\u0639\u062a\u0645\u0627\u062f \u0639\u0644\u0649 \u0628\u0631\u0646\u0627\u0645\u062c Ammonium sulfate calculator by <a href=\"http:\/\/encorbio.com\/\">\u00a9EnCor Biotechnology Inc.<\/a> \u00a0\u0628\u0625\u062f\u062e\u0627\u0644 \u0627\u0644\u062d\u062c\u0645 \u0627\u0644\u0628\u062f\u0626\u064a \u0644\u0644\u0645\u0635\u0644 \u0648\u0646\u0633\u0628\u0629 \u0625\u0634\u0628\u0627\u0639 \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0627\u0644\u0628\u062f\u0626\u064a\u0629\u060c \u0648\u0627\u0644\u0646\u0647\u0627\u0626\u064a\u0629\u060c \u0648\u062f\u0631\u062c\u0629 \u062d\u0631\u0627\u0631\u0629 \u0627\u0644\u062a\u0631\u0633\u064a\u0628 \u0644\u0644\u062d\u0635\u0648\u0644 \u0639\u0644\u0649 \u0643\u0645\u064a\u0629 \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0627\u0644\u0648\u0627\u062c\u0628 \u0625\u0636\u0627\u0641\u062a\u0647\u0627 \u0645\u0642\u062f\u0631\u0629 \u0628\u0627\u0644\u063a\u0631\u0627\u0645\u060c \u0648\u0627\u0644\u062d\u062c\u0645 \u0627\u0644\u0646\u0647\u0627\u0626\u064a \u0627\u0644\u0630\u064a \u0633\u0646\u062d\u0635\u0644 \u0639\u0644\u064a\u0647 \u0628\u0639\u062f \u0630\u0648\u0628\u0627\u0646 \u0643\u0627\u0645\u0644 \u0643\u0645\u064a\u0629 \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0627\u0644\u0645\u0636\u0627\u0641\u0629\u060c \u064a\u0639\u062a\u0645\u062f \u0627\u0644\u0628\u0631\u0646\u0627\u0645\u062c \u0644\u062d\u0633\u0627\u0628 \u0639\u062f\u062f \u063a\u0631\u0627\u0645\u0627\u062a \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0627\u0644\u0648\u0627\u062c\u0628 \u0625\u0636\u0627\u0641\u062a\u0647\u0627 \u0639\u0644\u0649 \u0627\u0644\u0645\u0639\u0627\u062f\u0644\u0629 \u0627\u0644\u062a\u0627\u0644\u064a\u0629:<\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: 'times new roman', times, serif;\"><em>G = Sat(M<sub>2<\/sub>-M<sub>1<\/sub>)\/(SatM-(SpecVol\/1000*132.14*SatM*M<sub>2<\/sub>))<\/em><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: 'times new roman', times, serif;\">G: \u0643\u0645\u064a\u0629 \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0644\u0648\u0627\u062c\u0628 \u0625\u0636\u0627\u0641\u062a\u0647\u0627 \u0644\u0643\u0644 1 \u0644\u062a\u0631<\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: 'times new roman', times, serif;\">Sat: \u0639\u062f\u062f \u0627\u0644\u063a\u0631\u0627\u0645\u0627\u062a \u0644\u0643\u0644 \u0644\u062a\u0631 \u0627\u0644\u0645\u0648\u062c\u0648\u062f\u0629 \u0641\u064a \u0645\u062d\u0644\u0648\u0644 \u0645\u0634\u0628\u0639 \u0644\u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645<\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: 'times new roman', times, serif;\">M2: \u0627\u0644\u0645\u0648\u0644\u0627\u0631\u064a\u0629 \u0627\u0644\u0645\u0631\u0627\u062f \u0627\u0644\u062d\u0635\u0648\u0644 \u0639\u0644\u064a\u0647\u0627<\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: 'times new roman', times, serif;\">M1: \u0627\u0644\u0645\u0648\u0644\u0627\u0631\u064a\u0629 \u0627\u0644\u0628\u062f\u0626\u064a\u0629<\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: 'times new roman', times, serif;\">SatM: \u0627\u0644\u0645\u0648\u0644\u0627\u0631\u064a\u0629 \u0644\u0645\u062d\u0644\u0648\u0644 \u0645\u0634\u0628\u0639 \u0645\u0646 \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645<\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: 'times new roman', times, serif;\">SpecVol: \u062d\u062c\u0645 \u0646\u0648\u0639\u064a \u0627\u0644\u0630\u064a \u062a\u0633\u0628\u0628 \u0625\u0636\u0627\u0641\u0629 1 \u063a \u0645\u0646 \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0632\u064a\u0627\u062f\u062a\u0647 \u0644\u0645\u062d\u0644\u0648\u0644 \u2248 0.54 \u0645\u0644<\/span><\/p>\n<p style=\"text-align: right;\"><span style=\"font-family: 'times new roman', times, serif;\">\u064a\u0623\u062e\u0630 \u0627\u0644\u0628\u0631\u0646\u0627\u0645\u062c \u0636\u0645\u0646 \u062d\u0633\u0627\u0628\u0627\u062a\u0647 \u062a\u063a\u064a\u0631 \u0643\u0644 \u0645\u0646 Sat\u060c SatM \u0648SpecVol \u0628\u062a\u063a\u064a\u0631 \u062f\u0631\u062c\u0629 \u062d\u0631\u0627\u0631\u0629 \u0627\u0644\u062d\u0636\u0646. <\/span><span style=\"font-family: 'times new roman', times, serif;\">\u064a\u062d\u0636\u0646 \u0627\u0644\u0623\u0646\u0628\u0648\u0628 \u0644\u0645\u062f\u0629 \u0633\u0627\u0639\u0629 \u0641\u064a \u0627\u0644\u062f\u0631\u062c\u0629 10 \u00b0 C\u0645\u0639 \u0627\u0644\u0631\u062c \u062b\u0645 \u064a\u0646\u0628\u0630 \u0628\u0633\u0631\u0639\u0629 10000\u00d7g \u0644\u0645\u062f\u0629 20 \u062f\u0642\u064a\u0642\u0629 \u0641\u064a \u062f\u0631\u062c\u0629 \u062d\u0631\u0627\u0631\u0629 4 \u00b0C. \u062a\u0648\u0632\u0646 \u0627\u0644\u0631\u0633\u0627\u0628\u0629 \u0641\u064a \u0643\u0644 \u062e\u0637\u0648\u0629 \u0645\u0646 \u062e\u0644\u0627\u0644 \u0637\u0631\u062d \u0648\u0632\u0646 \u0627\u0644\u0623\u0646\u0628\u0648\u0628 \u0627\u0644\u0641\u0627\u0631\u063a \u0645\u0646 \u0648\u0632\u0646 \u0627\u0644\u0623\u0646\u0628\u0648\u0628 \u0627\u0644\u0645\u062d\u062a\u0648\u064a \u0639\u0644\u0649 \u0627\u0644\u0631\u0633\u0627\u0628\u0629 \u0648\u062a\u062d\u0644 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062f\u0627\u0631\u0626\u0629 PBS pH=7.4 \u0628\u0645\u0642\u062f\u0627\u0631 1-2 \u0645\u0644 \u0644\u0643\u0644 1\u063a \u0631\u0627\u0633\u0628\u060c \u0648\u064a\u0633\u062d\u0628 \u0627\u0644\u0637\u0627\u0641\u064a \u0644\u064a\u0646\u0642\u0644 10 \u0645\u0644 \u0645\u0646\u0647 \u0625\u0644\u0649 \u0623\u0646\u0628\u0648\u0628 \u062c\u062f\u064a\u062f \u0648\u064a\u0639\u0627\u062f \u062a\u0631\u0633\u064a\u0628 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u062a\u0628\u0642\u064a\u0629 \u0641\u064a \u0627\u0644\u0645\u0635\u0644 \u0648\u0641\u0642 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\u062a\u0639\u0644\u064a\u0645\u0627\u062a \u0627\u0644\u0634\u0631\u0643\u0629 \u0627\u0644\u0645\u0646\u062a\u062c\u0629 \u062d\u062a\u0649 \u0646\u0647\u0627\u064a\u0629 \u0627\u0644\u0631\u062d\u0644\u0627\u0646. <\/span><span style=\"font-family: 'times new roman', times, serif;\">\u0628\u0639\u062f \u0627\u0644\u0627\u0646\u062a\u0647\u0627\u0621 \u0645\u0646 \u0627\u0644\u0631\u062d\u0644\u0627\u0646 \u0627\u0644\u0643\u0647\u0631\u0628\u0627\u0626\u064a \u0635\u0628\u063a\u062a \u0627\u0644\u0647\u0644\u0627\u0645\u0629 staining \u0628\u0627\u0644\u0637\u0631\u064a\u0642\u0629 \u0627\u0644\u0633\u0631\u064a\u0639\u0629\u060c \u0628\u0632\u064a\u0627\u062f\u0629 \u0627\u0644\u062d\u0633\u0627\u0633\u064a\u0629\u060c \u0628\u0631\u0641\u0639 \u062f\u0631\u062c\u0629 \u0627\u0644\u062d\u0631\u0627\u0631\u0629 \u0641\u064a \u0623\u062b\u0646\u0627\u0621 \u0627\u0644\u0635\u0628\u063a \u0648\u0625\u0632\u0627\u0644\u0629 \u0627\u0644\u0635\u0628\u0627\u063a [13]. \u063a\u0645\u0631\u062a \u0627\u0644\u0647\u0644\u0627\u0645\u0629 \u0641\u064a \u0627\u0644\u0635\u0628\u0627\u063a \u0645\u062f\u0629 5 \u062f\u0642\u0627\u0626\u0642 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 50 \u0645\u0644 \u0623\u0632\u0631\u0642 \u0627\u0644\u0643\u0648\u0645\u0627\u0632\u064a coomassie blue \u0628\u0639\u062f \u0627\u0644\u062a\u0633\u062e\u064a\u0646 \u0644\u0645\u062f\u0629 30 \u062b\u0627\u0646\u064a\u0629 \u0641\u064a \u0627\u0644\u0645\u0627\u064a\u0643\u0631\u0648\u064a\u0641 \u0648\u0645\u0646 \u062b\u0645 \u0623\u0632\u064a\u0644 \u0627\u0644\u0635\u0628\u0627\u063a destaining \u0628\u063a\u0645\u0631 \u0627\u0644\u0647\u0644\u0627\u0645\u0629 \u0641\u064a \u062d\u0648\u0636 \u064a\u062d\u0648\u064a 100 \u0645\u0644 \u0645\u062d\u0644\u0648\u0644 \u0625\u0632\u0627\u0644\u0629 \u0627\u0644\u0635\u0628\u0627\u063a \u0627\u0644\u062d\u0627\u0648\u064a \u0639\u0644\u0649 \u062d\u0645\u0636 \u0627\u0644\u062e\u0644 5% \u0648\u0645\u064a\u062a\u0627\u0646\u0648\u0644 10% \u0648\u0627\u0644\u062a\u0633\u062e\u064a\u0646 \u0641\u064a \u0627\u0644\u0645\u0627\u064a\u0643\u0631\u0648\u064a\u0641 \u0644\u0645\u062f\u0629 \u062f\u0642\u064a\u0642\u0629\u060c \u062b\u0645 \u062a\u062a\u0631\u0643 \u0633\u0627\u0639\u0629 \u0645\u0639 \u0627\u0644\u0631\u062c \u0625\u0644\u0649 \u062d\u064a\u0646 \u0638\u0647\u0648\u0631 \u0627\u0644\u0639\u0635\u0627\u0628\u0627\u062a \u0628\u0639\u062f \u0632\u0648\u0627\u0644 \u0627\u0644\u0644\u0648\u0646 \u0639\u0646 \u0628\u0627\u0642\u064a \u0627\u0644\u0647\u0644\u0627\u0645\u0629.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\"><strong>\u0627\u0644\u0643\u0634\u0641 \u0639\u0646 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0628\u062a\u0642\u0646\u064a\u0629 \u0644\u0637\u062e\u0629 \u0648\u064a\u0633\u062a\u0631\u0646 <\/strong><strong>western blot<\/strong><strong> \u0644\u0644\u0639\u064a\u0646\u0627\u062a \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u064a\u0629 \u0628\u0639\u062f \u062a\u0631\u062d\u064a\u0644\u0647\u0627<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\">\u062a\u0639\u062f \u0644\u0637\u062e\u0629 \u0648\u064a\u0633\u062a\u0631\u0646 \u0637\u0631\u064a\u0642\u0629 \u0645\u0641\u064a\u062f\u0629 \u062c\u062f\u0627\u064b \u0644\u0644\u0643\u0634\u0641 \u0627\u0644\u0646\u0648\u0639\u064a \u0639\u0646 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0628\u0639\u062f \u0641\u0635\u0644\u0647\u0627 \u0639\u0644\u0649 \u0647\u0644\u0627\u0645\u0629 \u0627\u0644\u0623\u0643\u0631\u064a\u0644\u0627\u0645\u064a\u062f \u062a\u0628\u0639\u0627\u064b \u0644\u0623\u0648\u0632\u0627\u0646\u0647\u0627 \u0627\u0644\u062c\u0632\u064a\u0626\u064a\u0629\u060c \u0641\u0636\u0644\u0627\u064b \u0639\u0646 \u0625\u0645\u0643\u0627\u0646\u064a\u0629 \u0627\u0644\u0643\u0634\u0641 \u0627\u0644\u0643\u0645\u064a \u0627\u0639\u062a\u0645\u0627\u062f\u0623 \u0639\u0644\u0649 \u0633\u0645\u0627\u0643\u0629 \u0627\u0644\u0639\u0635\u0627\u0628\u0629 \u0627\u0644\u062a\u064a \u062a\u0639\u0628\u0631 \u0639\u0646 \u0643\u0645\u064a\u0629 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 [14]. \u0631\u064c\u062d\u0644\u062a \u0627\u0644\u0639\u064a\u0646\u0627\u062a \u0641\u064a \u0627\u062e\u062a\u0628\u0627\u0631 \u0644\u0637\u062e\u0629 \u0648\u064a\u0633\u062a\u0631\u0646 \u0639\u0644\u0649 \u0647\u0644\u0627\u0645\u0629 \u0627\u0644\u0623\u0643\u0631\u064a\u0644\u0627\u0645\u064a\u062f\u060c \u0648\u0642\u0635\u062a \u0623\u0637\u0631\u0627\u0641 \u0627\u0644\u0647\u0644\u0627\u0645\u0629 \u0628\u0639\u062f \u0646\u0647\u0627\u064a\u0629 \u0627\u0644\u062a\u0631\u062d\u064a\u0644\u060c \u0648\u0645\u0646 \u062b\u0645 \u0648\u0636\u0639\u062a \u0639\u0644\u0649 \u063a\u0634\u0627\u0621 \u0644\u0637\u062e\u0629 \u0648\u064a\u0633\u062a\u0631\u0646 \u0628\u064a\u0646 \u0637\u0628\u0642\u0627\u062a \u0645\u0646 \u0623\u0648\u0631\u0627\u0642 \u0627\u0644\u062a\u0631\u0634\u064a\u062d \u0627\u0644\u0645\u0634\u0628\u0639\u0629 \u0628\u062f\u0627\u0631\u0626\u0629 \u0627\u0644\u0646\u0642\u0644 \u00a0transfer buffer (25 mM tris \u2212 base, 200 mM glycine, 0.1% SDS and 20% methanol)\u060c \u0648\u0636\u0639 \u0627\u0644\u0642\u0627\u0644\u0628 \u0628\u064a\u0646 \u0635\u0641\u064a\u062d\u062a\u064a \u062c\u0647\u0627\u0632 \u0627\u0644\u0646\u0642\u0644 \u0627\u0644\u0643\u0647\u0631\u0628\u0627\u0626\u064a TE 77 PWR semi-dry transter unit (GE lifescience) \u0628\u0647\u062f\u0641 \u0646\u0642\u0644 \u0627\u0644\u0639\u0635\u0627\u0628\u0627\u062a \u0645\u0646 \u0627\u0644\u0647\u0644\u0627\u0645\u0629 \u0625\u0644\u0649 \u063a\u0634\u0627\u0621 \u0646\u062a\u0631\u0648\u0633\u0644\u0648\u0644\u0648\u0632 0.45 \u0645\u0643\u0645 (Bio-Rad) \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062a\u064a\u0627\u0631 \u0643\u0647\u0631\u0628\u0627\u0626\u064a\u060c \u0637\u0628\u0642 \u062d\u0642\u0644 \u0643\u0647\u0631\u0628\u0627\u0626\u064a 100\u0623\u0645\u0628\u064a\u0631 \u0644\u0645\u062f\u0629 \u0633\u0627\u0639\u0629 \u0644\u0636\u0645\u0627\u0646 \u062a\u0645\u0627\u0645 \u0627\u0644\u0627\u0646\u062a\u0642\u0627\u0644\u060c \u0628\u0639\u062f \u0627\u0646\u062a\u0647\u0627\u0621 \u0627\u0644\u0648\u0642\u062a \u062d\u0631\u0631 \u0627\u0644\u063a\u0634\u0627\u0621 \u0645\u0646 \u0627\u0644\u0642\u0627\u0644\u0628 \u0648\u063a\u0633\u0644 \u0628\u062f\u0627\u0631\u0626\u0629 \u0627\u0644\u063a\u0633\u0644. <\/span><span style=\"font-family: 'times new roman', times, serif;\">\u062d\u0636\u0646 \u0627\u0644\u063a\u0634\u0627\u0621 \u0641\u064a \u0645\u062d\u0644\u0648\u0644 \u0627\u0644\u0625\u062d\u0635\u0627\u0631 Blocking (\u062d\u0644\u064a\u0628 \u0645\u0646\u0632\u0648\u0639 \u0627\u0644\u062f\u0633\u0645 5%) \u0644\u0645\u062f\u0629 \u0633\u0627\u0639\u062a\u064a\u0646 \u062b\u0645 \u063a\u0633\u0644 \u0645\u0631\u0627\u062a \u0639\u062f\u0629 \u0628\u0645\u062d\u0644\u0648\u0644 \u0627\u0644\u063a\u0633\u0644 (PBS pH=7.4 \u0627\u0644\u0645\u062d\u062a\u0648\u064a \u0639\u0644\u0649 \u062a\u0648\u064a\u0646 20 \u0628\u062a\u0631\u0643\u064a\u0632 0.05%). \u0627\u062e\u062a\u0628\u0631\u062a \u062b\u0644\u0627\u062b\u0629 \u0623\u0636\u062f\u0627\u062f \u0645\u062e\u062a\u0644\u0641\u0629 \u0628\u0627\u0644\u062a\u0645\u062f\u064a\u062f\u0627\u062a \u0627\u0644\u0645\u0646\u0627\u0633\u0628\u0629 \u0644\u0644\u0643\u0634\u0641 \u0639\u0646 \u0627\u0644\u063a\u0644\u0648\u0628\u0648\u0644\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 \u0639\u0644\u0649 \u0627\u0644\u063a\u0634\u0627\u0621 \u0648\u0647\u064a \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u0623\u0631\u0646\u0628 \u0627\u0644\u0645\u0645\u0646\u0639 \u0628\u0645\u0635\u0644 \u0627\u0644\u062c\u0645\u0644 R-a-camel (Bethyl Laboratories Inc.)\u060c \u0648\u0623\u0636\u062f\u0627\u062f \u0627\u0644\u0623\u0631\u0646\u0628 \u0627\u0644\u0645\u0645\u0646\u0639 \u0628\u0645\u0632\u064a\u062c \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 \u0645\u062d\u0636\u0631 \u0641\u064a \u0627\u0644\u0645\u062e\u0628\u0631 Home-made R-a-Nb \u0648\u0623\u0636\u062f\u0627\u062f \u0627\u0644\u0623\u0631\u0646\u0628 \u0627\u0644\u0645\u0645\u0646\u0639 \u0628\u0627\u0644\u063a\u0644\u0648\u0628\u0648\u0644\u064a\u0646\u0627\u062a \u062b\u0642\u064a\u0644\u0629 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 \u0645\u0646 \u0645\u0635\u0644 \u0627\u0644\u062c\u0645\u0644 \u0645\u062d\u0636\u0631 \u0641\u064a \u0627\u0644\u0645\u062e\u0628\u0631 (R-a-IgG2,3)\u060c \u0625\u0630 \u062d\u064c\u0636\u0646 \u063a\u0634\u0627\u0621 \u0627\u0644\u0646\u062a\u0631\u0648\u0633\u0644\u0648\u0644\u0648\u0632 \u0641\u064a \u062e\u0637\u0648\u0629 \u0644\u0627\u062d\u0642\u0629 \u0628\u0623\u0636\u062f\u0627\u062f \u0627\u0644\u0645\u0627\u0639\u0632 \u0627\u0644\u0645\u0645\u0646\u0639 \u0628\u0645\u0635\u0644 \u0627\u0644\u0623\u0631\u0646\u0628 \u0648\u0627\u0644\u0645\u062f\u0645\u062c\u0629 \u0645\u0639 \u0625\u0646\u0632\u064a\u0645 \u0627\u0644\u0628\u064a\u0631\u0648\u0643\u0633\u064a\u062f\u0627\u0632 G-a-R-HRP (Bethyl Laboratories Inc.) \u0648\u0645\u0646 \u062b\u0645 \u0623\u0636\u064a\u0641\u062a \u0631\u0643\u0627\u0632\u0629 \u0627\u0644\u0628\u064a\u0631\u0648\u0643\u0633\u064a\u062f\u0627\u0632 3-\u0623\u0645\u064a\u0646\u0648-9-\u0625\u064a\u062a\u064a\u0644 \u0643\u0627\u0631\u0628\u0627\u0632\u0648\u0644 (AEC) \u0627\u0644\u0645\u062d\u0636\u0631\u0629 \u0636\u0645\u0646 \u062f\u0627\u0631\u0626\u0629 \u0633\u064a\u062a\u0631\u0627\u062a \u0627\u0644\u0641\u0633\u0641\u0627\u062a pH=5 \u0645\u0639 \u0627\u0644\u0645\u0627\u0621 \u0627\u0644\u0623\u0643\u0633\u062c\u064a\u0646\u064a \u0628\u062a\u0631\u0643\u064a\u0632 \u0646\u0647\u0627\u0626\u064a 0.015% \u0644\u0625\u0638\u0647\u0627\u0631 \u0627\u0644\u0639\u0635\u0627\u0628\u0627\u062a.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\"><strong>\u0627\u0644\u0645\u0628\u0627\u062f\u0644\u0629 \u0627\u0644\u062f\u0627\u0631\u0626\u064a\u0629 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0643\u0631\u0648\u0645\u0627\u062a\u0648\u063a\u0631\u0627\u0641\u064a\u0627 \u0645\u0628\u0627\u062f\u0644\u0629 \u062f\u0627\u0631\u0626\u064a\u0629 <\/strong><strong>Buffer exchange chromatography<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\">\u062a\u0633\u062a\u062e\u062f\u0645 \u0627\u0644\u0645\u0628\u0627\u062f\u0644\u0629 \u0627\u0644\u062f\u0627\u0631\u0626\u064a\u0629 \u0628\u0647\u062f\u0641 \u0627\u0644\u062a\u062e\u0644\u0635 \u0645\u0646 \u0628\u0642\u0627\u064a\u0627 \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0641\u064a \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0627\u0644\u0630\u064a \u0631\u0633\u0628 \u0648\u063a\u064a\u0631\u062a \u0627\u0644\u062f\u0627\u0631\u0626\u0629 \u0627\u0644\u0645\u062d\u062a\u0648\u064a\u0629 \u0639\u0644\u0649 \u0627\u0644\u063a\u0644\u0648\u0628\u0648\u0644\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 [10]\u060c \u0627\u0633\u062a\u062e\u062f\u0645 \u062c\u0647\u0627\u0632 AKTA<sup>TM<\/sup> Pure 150 \u0628\u0639\u062f \u062a\u0631\u0643\u064a\u0628 \u0639\u0645\u0648\u062f HiPrep 26\/10 Desalting \u0645\u062d\u0634\u0648 \u0628\u062d\u0628\u064a\u0627\u062a \u0627\u0644\u0633\u064a\u0641\u0627\u062f\u0643\u0633 sephadex G-25 fine \u0630\u0627\u062a \u0627\u0644\u0642\u062f\u0631\u0629 \u0639\u0644\u0649 \u0641\u0635\u0644 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0628\u062e\u0627\u0635\u064a\u0629 \u0627\u0644\u0627\u0633\u062a\u0628\u0639\u0627\u062f \u0627\u0644\u062d\u062c\u0645\u064a \u0639\u0646 \u0627\u0644\u0634\u0648\u0627\u0631\u062f \u0648\u0627\u0644\u0645\u0643\u0648\u0646\u0627\u062a \u063a\u064a\u0631 \u0627\u0644\u0645\u0631\u063a\u0648\u0628 \u0628\u0647\u0627 \u0627\u0644\u062a\u064a \u0642\u062f \u062a\u0624\u062b\u0631 \u0633\u0644\u0628\u0627\u064b \u0639\u0641\u064a \u0637\u064a \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0648\u062b\u0628\u0627\u062a\u0647 \u0648\u0643\u0641\u0627\u0621\u0629 \u0627\u0644\u0641\u0635\u0644 \u0641\u064a \u0627\u0644\u062e\u0637\u0648\u0627\u062a \u0627\u0644\u062a\u0627\u0644\u064a\u0629. \u062d\u064a\u062b \u063a\u0633\u0644 \u0627\u0644\u0639\u0645\u0648\u062f \u0628\u0627\u0644\u0645\u0627\u0621 \u0627\u0644\u0645\u0642\u0637\u0631 \u0648\u0627\u0644\u0645\u0641\u0644\u062a\u0631 \u0643\u0645\u0627 \u0647\u0648 \u0627\u0644\u062d\u0627\u0644 \u0628\u0627\u0644\u0646\u0633\u0628\u0629 \u0625\u0644\u0649 \u062c\u0645\u064a\u0639 \u0627\u0644\u062f\u0648\u0627\u0631\u0626 \u0627\u0644\u0645\u0633\u062a\u062e\u062f\u0645\u0629 \u0641\u064a \u062a\u0642\u0646\u064a\u0627\u062a \u0643\u0631\u0648\u0645\u0627\u062a\u0648\u063a\u0631\u0627\u0641\u064a\u0627 \u0627\u0644\u062a\u0646\u0642\u064a\u0629 \u0644\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a\u060c \u0641\u0644\u062a\u0631\u062a \u0627\u0644\u062f\u0648\u0627\u0631\u0626 \u0648\u0627\u0644\u0645\u062d\u0627\u0644\u064a\u0644 \u0627\u0644\u0645\u0633\u062a\u062e\u062f\u0645\u0629 \u0628\u0645\u0627 \u0641\u064a \u0630\u0644\u0643 \u0627\u0644\u0639\u064a\u0646\u0627\u062a \u0627\u0644\u062d\u0627\u0648\u064a\u0629 \u0639\u0644\u0649 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0639\u0628\u0631 \u063a\u0634\u0627\u0621 \u0646\u062a\u0631\u0648\u0633\u0644\u0648\u0644\u0648\u0632 0.45 \u0645\u0643\u0631\u0648\u0645\u062a\u0631. \u0648\u0647\u064a\u0626 \u0627\u0644\u0639\u0645\u0648\u062f \u0628\u062f\u0627\u0631\u0626\u0629 \u0627\u0644\u0645\u0628\u0627\u062f\u0644\u0629 (\u062f\u0627\u0631\u0626\u0629 \u0641\u0633\u0641\u0627\u062a 20mM pH=7.4 + \u0643\u0644\u0648\u0631\u064a\u062f \u0627\u0644\u0635\u0648\u062f\u064a\u0648\u0645 (150 mM\u060c \u0645\u0631\u0631\u062a \u0627\u0644\u0639\u064a\u0646\u0629 \u0628\u062d\u062c\u0645 5 \u0645\u0644 \u0639\u0628\u0631 \u0627\u0644\u0639\u0645\u0648\u062f \u0628\u0648\u0627\u0633\u0637\u0629 \u0627\u0644\u062c\u0647\u0627\u0632 \u0628\u0633\u0631\u0639\u0629 \u062a\u062f\u0641\u0642 20 \u0645\u0644\\\u062f\u0642\u064a\u0642\u0629 \u0648\u062a\u064f\u062a\u0628\u0639 \u0645\u0631\u0648\u0631 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0639\u0628\u0631 \u0627\u0644\u0646\u0638\u0627\u0645 \u0648\u0639\u0645\u0648\u062f \u0627\u0644\u0645\u0628\u0627\u062f\u0644\u0629 \u0645\u0646 \u0642\u064a\u0627\u0633 \u0627\u0644\u0627\u0645\u062a\u0635\u0627\u0635\u064a\u0629 \u0641\u064a \u0645\u0648\u062c\u0629 \u0637\u0648\u0644\u0647\u0627 280 \u0646\u0645\u060c \u062c\u0645\u0639\u062a \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0628\u0639\u062f \u0639\u0645\u0644\u064a\u0629 \u0627\u0644\u0645\u0628\u0627\u062f\u0644\u0629 \u0648\u0631\u0643\u0632\u062a \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0623\u0646\u0627\u0628\u064a\u0628 \u0627\u0644\u062a\u0631\u0643\u064a\u0632 vivaspin 10000 kDa.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\"><strong>\u062a\u0646\u0642\u064a\u0629 \u0627\u0644\u063a\u0644\u0648\u0628\u0648\u0644\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0643\u0631\u0648\u0645\u0627\u062a\u0648\u063a\u0631\u0627\u0641\u064a\u0627 \u0627\u0644\u0623\u0644\u0641\u0629<\/strong> <strong>Affinity chromatography<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\">\u0627\u0633\u062a\u062e\u062f\u0645 \u0639\u0645\u0648\u062f \u0627\u0644\u0641\u0635\u0644 5 ml HiTrap Protein A column (Pharmacia) \u0628\u063a\u064a\u0629 \u0627\u0644\u062a\u062e\u0644\u0635 \u0645\u0646 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0627\u0644\u0623\u062e\u0631\u0649 \u0627\u0644\u062a\u064a \u0631\u064f\u0633\u0651\u0650\u0628\u062a \u0628\u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0645\u0639 \u0627\u0644\u063a\u0644\u0648\u0628\u0648\u0644\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 \u0644\u0643\u0648\u0646 \u0627\u0644\u0639\u0645\u0648\u062f \u0645\u0645\u064a\u0632\u0627\u064b \u0628\u0625\u0644\u0641\u062a\u0647 \u0644\u0644\u063a\u0648\u0628\u0648\u0644\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 IgG\u060c \u0625\u0630 \u0625\u0646\u0647\u0627 \u062a\u0631\u062a\u0628\u0637 \u0645\u0639 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 A \u0645\u0646 \u062e\u0644\u0627\u0644 \u062b\u0644\u0645 \u0643\u0627\u0631\u0647 \u0644\u0644\u0645\u0627\u0621 \u0628\u064a\u0646 \u0627\u0644\u0645\u0646\u0637\u0642\u062a\u064a\u0646 \u0627\u0644\u062b\u0627\u0646\u064a\u0629 CH2 \u0648\u0627\u0644\u062b\u0627\u0644\u062b\u0629 CH3 \u0639\u0644\u0649 \u0627\u0644\u062c\u0632\u0621 \u0627\u0644\u062b\u0627\u0628\u062a \u0645\u0646 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 \u0627\u0644\u062b\u0642\u064a\u0644\u0629 [15]\u060c \u063a\u0633\u0644 \u0627\u0644\u0639\u0645\u0648\u062f \u0628\u062f\u0627\u064a\u0629 \u0628\u0627\u0644\u0645\u0627\u0621 \u0627\u0644\u0645\u0642\u0637\u0631 \u0648\u0627\u0644\u0645\u0639\u0642\u0645\u060c \u0648\u0645\u0646 \u062b\u0645 \u0645\u0631\u0631 25 \u0645\u0644 \u0645\u0646 \u062f\u0627\u0631\u0626\u0629 \u0627\u0644\u0627\u0631\u062a\u0628\u0627\u0637 \u0648\u062d\u0642\u0646 5 \u0645\u0644 \u0645\u0646 \u0627\u0644\u0639\u064a\u0646\u0629 \u0628\u0648\u062c\u0648\u062f \u062f\u0627\u0631\u0626\u0629 \u0627\u0644\u0627\u0631\u062a\u0628\u0627\u0637 Binding buffer (20 mM sodium phosphate pH = 7.0)\u00a0 \u0628\u062d\u064a\u062b \u0623\u062a\u0627\u062d \u0627\u0631\u062a\u0628\u0627\u0637 \u0627\u0644\u063a\u0644\u0648\u0628\u0648\u0644\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 \u0630\u0627\u062a \u0627\u0644\u0623\u0644\u0641\u0629 \u0644\u0644\u0628\u0631\u0648\u062a\u064a\u0646 A \u0628\u0627\u0644\u0639\u0645\u0648\u062f \u0648\u0633\u0645\u062d \u0644\u0628\u0627\u0642\u064a \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0628\u0627\u0644\u062a\u062f\u0641\u0642 \u0644\u062a\u062c\u0645\u0639 \u0641\u064a \u0623\u0646\u0628\u0648\u0628 \u0644\u0644\u062a\u0631\u062d\u064a\u0644 \u0641\u064a \u062e\u0637\u0648\u0629 \u0644\u0627\u062d\u0642\u0629\u060c \u0648\u0645\u0646 \u062b\u0645 \u0627\u0633\u062a\u062e\u062f\u0645\u062a \u062f\u0627\u0631\u0626\u0629 \u0627\u0644\u0634\u0637\u0641 elution buffer (0.15 M NaCl, 0.58 % acetic acid pH = 4) \u0627\u0644\u062a\u064a \u062a\u0636\u0645\u0646 \u0641\u0643 \u0627\u0631\u062a\u0628\u0627\u0637 \u0627\u0644\u063a\u0644\u0648\u0628\u0648\u0644\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 \u0628\u0627\u0644\u0639\u0645\u0648\u062f. \u0623\u062e\u0630\u062a \u0639\u064a\u0646\u0627\u062a \u0644\u0644\u062a\u0631\u062d\u064a\u0644 \u0645\u0646 \u0643\u0644 \u0639\u064a\u0646\u0629 \u062c\u064c\u0645\u0639\u062a \u0639\u0646\u062f \u0638\u0647\u0648\u0631 \u0642\u0645\u0629 \u0639\u0644\u0649 \u0643\u0631\u0648\u0645\u0627\u062a\u0648\u063a\u0631\u0627\u0645 \u0627\u0644\u0627\u0645\u062a\u0635\u0627\u0635\u064a\u0629 (280 \u0646\u0645) \u0628\u0647\u062f\u0641 \u0643\u0634\u0641 \u0645\u062d\u062a\u0648\u0649 \u0647\u0630\u0647 \u0627\u0644\u0639\u064a\u0646\u0627\u062a \u0645\u0646 \u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0645\u0635\u0644 \u0627\u0644\u062c\u0645\u0644 \u0648\u0643\u0634\u0641 \u0643\u0641\u0627\u0621\u0629 \u0627\u0644\u062a\u0646\u0642\u064a\u0629 \u0644\u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\"><strong>\u0641\u0635\u0644 \u0623\u0646\u0645\u0627\u0637 <\/strong><strong>\u0627\u0644\u063a\u0644\u0648\u0628\u0648\u0644\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 \u0644\u0644\u062c\u0645\u0644<\/strong> <strong>\u0628<\/strong><strong>\u062a\u0642\u0646\u064a\u0629 \u0643\u0631\u0648\u0645\u0627\u062a\u0648\u063a\u0631\u0627\u0641\u064a\u0627 \u0627\u0644\u0627\u0633\u062a\u0628\u0639\u0627\u062f \u0627\u0644\u062d\u062c\u0645\u064a <\/strong><strong>Size exclusion chromatography (SEC)<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\">\u0627\u0633\u062a\u062e\u062f\u0645 \u0639\u0645\u0648\u062f Superdex 200 \u0644\u0641\u0635\u0644 \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u0630\u0627\u062a \u0627\u0644\u0633\u0644\u0633\u0644\u0629 \u0627\u0644\u062b\u0642\u064a\u0644\u0629 \u0639\u0646 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062a\u0642\u0644\u064a\u062f\u064a\u0629\u060c \u0625\u0630 \u062c\u0631\u0649 \u0627\u0639\u062a\u0645\u0627\u062f \u062f\u0627\u0631\u0626\u0629 \u063a\u0633\u0644\u00a0 washing buffer (20 mM sodium phosphate + 150 mM sodium chloride pH = 7.4) \u0628\u0639\u062f \u0623\u0646 \u063a\u064f\u0633\u0644 \u0627\u0644\u0639\u0645\u0648\u062f \u0628\u0636\u0639\u0641\u064a \u062d\u062c\u0645\u0647 \u0628\u0627\u0644\u0645\u0627\u0621 \u0627\u0644\u0645\u0642\u0637\u0631 \u0628\u0645\u0639\u062f\u0644 \u062a\u062f\u0641\u0642 1 \u0645\u0644\/\u062f\u0642\u064a\u0642\u0629 \u0648\u0645\u0646 \u062b\u0645 \u0645\u0631\u0631\u062a \u062f\u0627\u0631\u0626\u0629 \u0627\u0644\u063a\u0633\u0644 \u0644\u0645\u062f\u0629 \u0633\u0627\u0639\u062a\u064a\u0646 \u0628\u0645\u0639\u062f\u0644 \u0627\u0644\u062a\u062f\u0641\u0642 \u0630\u0627\u062a\u0647\u060c \u062b\u0645 \u0645\u064f\u0631\u0631\u062a \u0639\u064a\u0646\u0629 \u0627\u0644\u063a\u0644\u0648\u0628\u0648\u0644\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 \u0627\u0644\u0646\u0642\u064a\u0629 \u0627\u0644\u0646\u0627\u062a\u062c\u0629 \u0639\u0646 \u0639\u0645\u0644\u064a\u0629 \u0627\u0644\u062a\u0646\u0642\u064a\u0629 \u0627\u0644\u0633\u0627\u0628\u0642\u0629 \u0628\u0639\u062f \u0623\u0646 \u0631\u0643\u0632\u062a \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0623\u0646\u0628\u0648\u0628 vivaspin 10000 kDa \u0625\u0644\u0649 \u062d\u064a\u0646 \u0648\u0635\u0648\u0644 \u0627\u0644\u0639\u064a\u0646\u0629 \u0625\u0644\u0649 \u062d\u062c\u0645 500 \u0645\u0643\u0644 \u0648\u0628\u062a\u0631\u0643\u064a\u0632 \u0644\u0627 \u064a\u0632\u064a\u062f \u0639\u0646 10 \u0645\u063a\/500 \u0645\u0643\u0644\u060c \u062d\u0642\u0646\u062a \u0627\u0644\u0639\u064a\u0646\u0629 \u0628\u0639\u062f \u062a\u0645\u0631\u064a\u0631\u0647\u0627 \u0639\u0644\u0649 \u0641\u0644\u062a\u0631 0.22 \u0645\u0643\u0631\u0648\u0645\u062a\u0631 \u0648\u062c\u0645\u0639\u062a \u0639\u064a\u0646\u0627\u062a \u0639\u0646\u062f \u0643\u0644 \u0642\u0645\u0629 \u0627\u0645\u062a\u0635\u0627\u0635\u064a\u0629 \u062a\u0638\u0647\u0631 \u0639\u0644\u0649 \u0643\u0631\u0648\u0645\u0627\u062a\u0648\u063a\u0631\u0627\u0645 \u0627\u0644\u0627\u0645\u062a\u0635\u0627\u0635\u064a\u0629 \u0644\u062a\u0631\u062d\u064a\u0644\u0647\u0627 \u0641\u064a \u062e\u0637\u0648\u0629 \u0644\u0627\u062d\u0642\u0629. \u0627\u0633\u062a\u062e\u062f\u0645 \u0644\u0647\u0630\u0647 \u0627\u0644\u062a\u0642\u0627\u0646\u0629 AKTA explorer FPLC system. \u0631\u064f\u062d\u0651\u0650\u0644 5 \u0645\u0643\u0631\u0648\u063a\u0631\u0627\u0645 \u0645\u0646 \u0643\u0644 \u0639\u064a\u0646\u0629 \u062c\u0645\u0639\u062a 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Scientific, Wellwash \u0648\u0643\u0631\u0631\u062a \u0639\u0645\u0644\u064a\u0629 \u0627\u0644\u063a\u0633\u0644 \u0628\u0639\u062f \u0643\u0644 \u062e\u0637\u0648\u0629 \u062d\u0636\u0646. \u0641\u064a \u062d\u064a\u0646 \u063a\u0637\u064a\u062a \u0645\u0648\u0627\u0642\u0639 \u0627\u0631\u062a\u0628\u0627\u0637 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0627\u0644\u0623\u062e\u0631\u0649 \u0639\u0644\u0649 \u0633\u0637\u062d \u0627\u0644\u0622\u0628\u0627\u0631 \u0628\u0645\u062d\u0644\u0648\u0644 \u0627\u0644\u0625\u062d\u0635\u0627\u0631 \u0627\u0644\u0645\u0643\u0648\u0646 \u0645\u0646 \u062d\u0644\u064a\u0628 \u0645\u0642\u0634\u0648\u062f 5% \u0641\u064a \u062f\u0627\u0631\u0626\u0629 \u0641\u0633\u0641\u0627\u062a \u0645\u0644\u062d\u064a\u0629 (5\u00a0% skimmed milk in PBS pH=7.4) \u0645\u0639 \u0627\u0644\u062d\u0636\u0646 \u0644\u0645\u062f\u0629 \u0633\u0627\u0639\u0629 \u0628\u062f\u0631\u062c\u0629 \u062d\u0631\u0627\u0631\u0629 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\u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0627\u0644\u0645\u0635\u0644 \u0627\u0644\u0645\u0648\u0627\u0641\u0642 \u0648\u0647\u0648 \u0645\u0635\u0644 \u0627\u0644\u0623\u0631\u0646\u0628 \u0627\u0644\u062d\u0627\u0648\u064a \u0639\u0644\u0649 \u0623\u0636\u062f\u0627\u062f \u0646\u0648\u0639\u064a\u0629 \u0644\u0644\u0623\u0636\u062f\u0627\u062f \u062b\u0642\u064a\u0644\u0629 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 Rabbit anti-IgG2,3 \u0628\u062a\u0631\u0643\u064a\u0632 (1:200) \u0627\u0644\u062a\u064a \u0623\u062b\u0628\u062a\u062a \u0646\u0648\u0639\u064a\u062a\u0647\u0627 \u0641\u064a \u0627\u062e\u062a\u0628\u0627\u0631 \u0627\u0644\u062a\u0628\u0635\u064a\u0645 \u0627\u0644\u0645\u0646\u0627\u0639\u064a. \u0623\u0636\u064a\u0641\u062a \u0623\u0645\u0635\u0627\u0644 \u0645\u062d\u062a\u0648\u064a\u0629 \u0639\u0644\u0649 \u0623\u0636\u062f\u0627\u062f \u0645\u0627\u0639\u0632 \u0645\u0645\u0646\u0639 \u0628\u0645\u0635\u0644 \u0627\u0644\u0623\u0631\u0646\u0628 \u0645\u0648\u0633\u0648\u0645\u0629 \u0628\u0627\u0644\u0628\u064a\u0631\u0648\u0643\u0633\u064a\u062f\u0627\u0632 \u0628\u062a\u0645\u062f\u064a\u062f (1:3000) Gout anti rabbit-HRB (Bethyl Laboratories Inc.) \u00a0\u0645\u0639 \u0627\u0644\u062d\u0636\u0646 \u0645\u062f\u0629 \u0633\u0627\u0639\u0629 \u0641\u064a \u062f\u0631\u062c\u0629 \u062d\u0631\u0627\u0631\u0629 \u0627\u0644\u0645\u062e\u062a\u0628\u0631. \u0641\u064a \u0627\u0644\u062e\u0637\u0648\u0629 \u0627\u0644\u0623\u062e\u064a\u0631\u0629 \u0623\u0636\u064a\u0641 \u0627\u0644\u0645\u062f\u0627\u062f \u0627\u0644\u062e\u0627\u0635 \u0628\u0627\u0644\u0628\u064a\u0631\u0648\u0643\u0633\u064a\u062f\u0627\u0632 3,3\u2032,5,5\u2032-Tetramethylbenzidine (TMB) (Sigma) \u0628\u0639\u062f\u0647\u0627 \u0623\u0648\u0642\u0641 \u0627\u0644\u062a\u0641\u0627\u0639\u0644 \u0628\u0627\u0633\u062a\u0639\u0645\u0627\u0644 \u062d\u0645\u0636 \u0627\u0644\u0643\u0628\u0631\u064a\u062a 0.5 M \u062b\u0645 \u0642\u064a\u0633\u062a \u0627\u0644\u0645\u0637\u064a\u0627\u0641\u064a\u0629 \u0627\u0644\u0646\u0627\u062a\u062c\u0629 \u0648\u0630\u0644\u0643 \u0641\u064a \u0645\u0648\u062c\u0629 \u0637\u0648\u0644\u0647\u0627 450 \u0646\u0627\u0646\u0648\u0645\u062a\u0631 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0642\u0627\u0631\u0626 \u0635\u0641\u0627\u0626\u062d Thermo Scientific, Multiskan Go. \u0627\u0639\u062a\u0645\u062f \u0627\u062e\u062a\u0628\u0627\u0631 one-way ANOVA \u0644\u0645\u0642\u0627\u0631\u0646\u0629 \u062b\u0628\u0627\u062a \u0623\u0646\u0645\u0627\u0637 \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 IgG \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 Prism v7.0 software (GraphPad Statistics, USA)\u060c *p&lt;0.05.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\"><strong>\u0627\u0644\u0646\u062a\u0627\u0626\u062c<\/strong> <strong>Results<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\"><strong>\u062a\u0631\u0633\u064a\u0628 \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u0628\u0645\u0644\u062d \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\">\u0627\u0646\u0637\u0644\u0642\u0646\u0627 \u0641\u064a \u0647\u0630\u0647 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0645\u0646 \u0639\u064a\u0646\u0629 \u0645\u0635\u0644 \u062f\u0645 \u0645\u0646 \u062c\u0645\u0644 \u0645\u0645\u0646\u0639 \u0628\u0633\u0645 \u0627\u0644\u0639\u0642\u0631\u0628\u060c \u0625\u0630 \u0627\u062e\u062a\u0628\u0631\u062a \u0627\u0644\u0634\u0631\u0648\u0637 \u0627\u0644\u0645\u062b\u0644\u0649 \u0645\u0646 \u062f\u0631\u062c\u0629 \u0625\u0634\u0628\u0627\u0639 \u0628\u0645\u0644\u062d \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0627\u0644\u0636\u0631\u0648\u0631\u064a\u0629 \u0644\u062a\u0631\u0633\u064a\u0628 \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u0628\u0646\u0648\u0639\u064a\u0647\u0627 \u0627\u0644\u062a\u0642\u0644\u064a\u062f\u064a\u0629 \u0648\u0627\u0644\u062b\u0642\u064a\u0644\u0629 \u0627\u0644\u0633\u0644\u0633\u0644\u0629\u060c \u0648\u0642\u062f \u0627\u0633\u062a\u062e\u062f\u0645\u062a \u0644\u0647\u0630\u0647 \u0627\u0644\u063a\u0627\u064a\u0629 \u0639\u062f\u0629 \u0646\u0633\u0628 \u0645\u0646 \u0627\u0644\u0625\u0634\u0628\u0627\u0639. \u0631\u064f\u0633\u0645 \u0627\u0644\u062e\u0637 \u0627\u0644\u0645\u0633\u062a\u0642\u064a\u0645 \u0644\u0642\u0631\u0627\u0621\u0627\u062a \u0627\u0644\u0633\u0644\u0633\u0644\u0629 \u0627\u0644\u0645\u0639\u064a\u0627\u0631\u064a\u0629 \u0644\u0623\u0644\u0628\u0648\u0645\u064a\u0646 \u0645\u0635\u0644 \u0627\u0644\u0628\u0642\u0631 BSA \u0636\u0645\u0646 \u0645\u062c\u0627\u0644 \u062e\u0637\u064a \u0645\u0646 \u0627\u0644\u062a\u0631\u0627\u0643\u064a\u0632 \u0628\u0648\u0627\u062d\u062f\u0629 \u0645\u063a\/\u0645\u0644 \u0627\u0644\u0630\u064a \u0639\u0628\u0631 \u0639\u0646\u0647 \u0628\u0645\u0639\u0627\u062f\u0644\u0629 \u064a\u0645\u0643\u0646 \u0645\u0646\u0647\u0627 \u0645\u0639\u0631\u0641\u0629 \u0627\u0644\u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0645\u062c\u0647\u0648\u0644 \u0644\u0644\u0623\u0636\u062f\u0627\u062f \u0644\u0643\u0644 \u0639\u064a\u0646\u0629 \u0628\u0639\u062f \u0645\u0639\u0631\u0641\u0629 \u0648\u0633\u0637\u064a \u0642\u0631\u0627\u0621\u0627\u062a \u0627\u0644\u0627\u0645\u062a\u0635\u0627\u0635\u064a\u0629 \u0644\u0644\u0645\u0643\u0631\u0631\u064a\u0646 \u0648\u0636\u0631\u0628 \u0627\u0644\u0646\u0627\u062a\u062c \u0628\u0645\u0639\u0627\u0645\u0644 \u0627\u0644\u062a\u0645\u062f\u064a\u062f \u0627\u0644\u062e\u0627\u0635 \u0628\u0627\u0644\u0639\u064a\u0646\u0629 (\u0643\u0645\u0627 \u0647\u0648 \u0645\u0648\u0636\u062d \u0641\u064a \u0627\u0644\u0634\u0643\u0644 1\u060c a)\u060c \u0643\u0645\u0627 \u062d\u0633\u0628 \u0648\u0632\u0646 \u0627\u0644\u0631\u0627\u0633\u0628 \u0627\u0644\u0646\u0627\u062a\u062c \u0641\u064a \u0643\u0644 \u0645\u0631\u062d\u0644\u0629 \u0648\u062d\u0644\u0647 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 PBS\u060c \u0648\u0645\u0646 \u062b\u0645 \u062d\u0633\u0628 \u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0639\u0646 \u0637\u0631\u064a\u0642 \u0627\u0644\u0645\u0639\u0627\u062f\u0644\u0629 \u0622\u0646\u0641\u0629 \u0627\u0644\u0630\u0643\u0631 (\u0643\u0645\u0627 \u0647\u0648 \u0645\u0648\u0636\u062d \u0641\u064a \u0627\u0644\u0634\u0643\u0644 1\u060c b)<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-453\" src=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2022\/10\/1-1-300x155.jpg\" alt=\"\" width=\"623\" height=\"322\" srcset=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2022\/10\/1-1-300x155.jpg 300w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2022\/10\/1-1-1024x531.jpg 1024w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2022\/10\/1-1-768x398.jpg 768w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2022\/10\/1-1.jpg 1428w\" sizes=\"auto, (max-width: 623px) 100vw, 623px\" \/><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\"><em><strong><span style=\"font-size: 10pt;\">(a) \u064a\u0638\u0647\u0631 \u0627\u0644\u0634\u0643\u0644 \u062e\u0637\u064a\u0629 \u0636\u0645\u0646 \u0645\u062c\u0627\u0644 \u062a\u0631\u0627\u0643\u064a\u0632 \u0645\u0646 \u0623\u0644\u0628\u0648\u0645\u064a\u0646 \u0627\u0644\u0645\u0635\u0644 \u0627\u0644\u0628\u0642\u0631\u064a BSA \u0645\u0639 \u0645\u0639\u0627\u0645\u0644 \u062a\u0635\u062d\u064a\u062d R2= 0.996\u060c \u062d\u064a\u062b \u0627\u0633\u062a\u062e\u062f\u0645\u062a \u0635\u0641\u064a\u062d\u0629 \u0632\u0631\u0627\u0639\u0629 \u062e\u0644\u0648\u064a\u0629 96 \u0628\u0626\u0631\u0627\u064b \u0648\u0648\u0636\u0639 5 \u0645\u0643\u0644 \u0636\u0645\u0646 \u0628\u0626\u0631\u064a\u0646 \u0645\u062a\u062c\u0627\u0648\u0631\u064a\u0646 \u0645\u0646 \u0643\u0644 \u0645\u0646 \u062a\u0631\u0627\u0643\u064a\u0632 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 (0\u060c 0.2\u060c 0.4\u060c 0.6\u060c 0.8 \u0645\u063a\/\u0645\u0644) \u0645\u0639 \u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062f\u0627\u0631\u0626\u0629 \u0627\u0644\u0641\u0648\u0633\u0641\u0627\u062a \u0627\u0644\u0645\u0644\u062d\u064a PBS \u0627\u0644\u0645\u0633\u062a\u062e\u062f\u0645 \u0644\u062a\u0645\u062f\u064a\u062f \u0627\u0644\u0639\u064a\u0646\u0627\u062a \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u064a\u0629 \u0643\u0634\u0627\u0647\u062f\u060c \u0623\u0636\u064a\u0641 160\u0645\u0643\u0644 \u0645\u0646 \u0643\u0627\u0634\u0641 \u0628\u0631\u0627\u062f\u0641\u0648\u0631\u062f \u0648\u0642\u0631\u0626\u062a \u0627\u0644\u0627\u0645\u062a\u0635\u0627\u0635\u064a\u0629 \u0628\u062c\u0647\u0627\u0632 thermos-scientific multiskan go \u0641\u064a \u0645\u0648\u062c\u0629 \u0637\u0648\u0644\u0647\u0627 595 \u0646\u0645 \u0648\u0631\u064f\u0633\u0645 \u0627\u0644\u062e\u0637 \u0627\u0644\u0645\u0633\u062a\u0642\u064a\u0645 \u0627\u0644\u0645\u0639\u0628\u0631 \u0639\u0646 \u062e\u0637\u064a\u0629 \u0627\u0644\u0637\u0631\u064a\u0642\u0629 \u0628\u0648\u0627\u0633\u0637\u0629 \u064a\u0631\u0646\u0627\u0645\u062c Skanlt software 4.1 for microplate readers\u060c (b) \u062c\u062f\u0648\u0644 \u0645\u0631\u0627\u062d\u0644 \u0625\u0636\u0627\u0641\u0629 \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0644\u0644\u0645\u0635\u0644 \u0644\u0644\u0648\u0635\u0648\u0644 \u0625\u0644\u0649 \u062f\u0631\u062c\u0627\u062a \u0627\u0644\u0625\u0634\u0628\u0627\u0639 \u0627\u0644\u0645\u0631\u0627\u062f \u0627\u062e\u062a\u0628\u0627\u0631\u0647\u0627 \u0628\u0648\u0635\u0641\u0647\u0627 \u0645\u0631\u0627\u062d\u0644 \u062a\u0631\u0633\u064a\u0628 \u0644\u0645\u0635\u0644 \u0627\u0644\u062c\u0645\u0644 \u0641\u064a \u062f\u0631\u062c\u0629 \u062d\u0631\u0627\u0631\u0629 10 \u00b0C\u060c \u0648\u0648\u0632\u0646 \u0627\u0644\u0631\u0627\u0633\u0628 \u0627\u0644\u0646\u0627\u062a\u062c \u0641\u064a \u0643\u0644 \u0645\u0631\u062d\u0644\u0629\u060c \u0641\u0636\u0644\u0627\u064b \u0639\u0646 \u0627\u0644\u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0646\u0647\u0627\u0626\u064a \u0628\u0639\u062f \u062d\u0644 \u0627\u0644\u0631\u0633\u0627\u0628\u0629 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 PBS \u0648\u0627\u0644\u0645\u062d\u0633\u0648\u0628 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0645\u0639\u0627\u062f\u0644\u0629 \u0627\u0644\u062e\u0637\u064a\u0629 \u0644\u0633\u0644\u0633\u0644\u0629 \u062a\u0631\u0627\u0643\u064a\u0632 \u0627\u0644\u0623\u0644\u0628\u0648\u0645\u064a\u0646 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0643\u0627\u0634\u0641 \u0628\u0631\u0627\u062f\u0641\u0648\u0631\u062f.<\/span><\/strong><\/em><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\"><strong>\u0627\u0644\u0631\u062d\u0644\u0627\u0646 \u0627\u0644\u0643\u0647\u0631\u0628\u0627\u0626\u064a \u0644\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0648\u0627\u0644\u062a\u0628\u0635\u064a\u0645 \u0627\u0644\u0645\u0646\u0627\u0639\u064a<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\">\u0638\u0647\u0631\u062a \u0639\u0644\u0649 \u0647\u0644\u0627\u0645\u0629 \u0627\u0644\u0623\u0643\u0631\u064a\u0644\u0627\u0645\u064a\u062f \u0628\u0639\u062f \u0635\u0628\u063a\u0647\u0627 \u0628\u0623\u0632\u0631\u0642 \u0627\u0644\u0643\u0648\u0645\u0627\u0632\u064a \u0627\u0644\u0639\u0635\u0627\u0628\u0627\u062a \u0627\u0644\u062e\u0627\u0635\u0629 \u0628\u0643\u0644 \u0645\u0646 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062a\u0642\u0644\u064a\u062f\u064a\u0629 \u0648\u062b\u0642\u064a\u0644\u0629 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 \u0628\u0645\u0627 \u064a\u062a\u0648\u0627\u0641\u0642 \u0623\u0643\u062b\u0631 \u0645\u0639 \u0627\u0644\u0634\u0627\u0647\u062f \u0627\u0644\u0625\u064a\u062c\u0627\u0628\u064a \u0627\u0644\u0623\u0648\u0644 \u0627\u0644\u0645\u062d\u062a\u0648\u064a \u0639\u0644\u0649 \u0645\u0632\u064a\u062c \u0645\u0646 \u0627\u0644\u063a\u0644\u0648\u0628\u0648\u0644\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 \u0627\u0644\u0645\u0646\u0642\u0627\u0629 \u0645\u0646 \u0645\u0635\u0644 \u0627\u0644\u062c\u0645\u0644 \u0639\u0646\u062f \u0627\u0644\u062a\u0631\u0633\u064a\u0628 \u0628\u062a\u0631\u0643\u064a\u0632 \u0625\u0634\u0628\u0627\u0639\u064a 20% \u0645\u0646 \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645\u060c \u0648\u0639\u0635\u0627\u0628\u0627\u062a \u062e\u0627\u0635\u0629 \u0628\u0627\u0644\u0623\u0636\u062f\u0627\u062f \u062b\u0642\u064a\u0644\u0629 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 \u0641\u0642\u0637 \u0644\u0644\u0639\u064a\u0646\u0629 \u0627\u0644\u062a\u064a \u062e\u0636\u0639\u062a \u0644\u0631\u0641\u0639 \u0627\u0644\u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0625\u0634\u0628\u0627\u0639\u064a \u0645\u0646 \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0645\u0646 20% \u0625\u0644\u0649 40%\u060c \u0628\u0645\u0627 \u064a\u062a\u0648\u0627\u0641\u0642 \u0645\u0639 \u0627\u0644\u0634\u0627\u0647\u062f \u0627\u0644\u0625\u064a\u062c\u0627\u0628\u064a \u0627\u0644\u062b\u0627\u0646\u064a \u0627\u0644\u0645\u062d\u062a\u0648\u064a \u0639\u0644\u0649 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u062b\u0642\u064a\u0644\u0629 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 IgG2,3 \u0627\u0644\u0646\u0642\u064a\u0629 \u0627\u0644\u0645\u062d\u0636\u0631\u0629 \u0641\u064a \u062f\u0631\u0627\u0633\u0627\u062a \u0633\u0627\u0628\u0642\u0629[17]\u060c \u0643\u0645\u0627 \u0644\u0648\u062d\u0638 \u0638\u0647\u0648\u0631 \u0627\u0644\u0639\u0635\u0627\u0628\u0629 \u0627\u0644\u062e\u0627\u0635\u0629 \u0628\u0623\u0644\u0628\u0648\u0645\u064a\u0646 \u0645\u0635\u0644 \u0627\u0644\u062c\u0645\u0644 \u0628\u0637\u0648\u0644 \u2248 90 \u0643\u064a\u0644\u0648 \u062f\u0627\u0644\u062a\u0648\u0646 \u0639\u0646\u062f \u0643\u0644 \u0627\u0644\u062a\u0631\u0627\u0643\u064a\u0632 \u0627\u0644\u0625\u0634\u0628\u0627\u0639\u064a\u0629 \u0627\u0644\u0645\u062c\u0631\u0628\u0629 \u0645\u0639 \u0645\u0644\u0627\u062d\u0638\u0629 \u0632\u064a\u0627\u062f\u0629 \u0643\u062b\u0627\u0641\u0629 \u0627\u0644\u0639\u0635\u0627\u0628\u0629 \u0628\u0627\u0644\u062a\u0631\u0627\u0643\u064a\u0632 \u0627\u0644\u0625\u0634\u0628\u0627\u0639\u064a\u0629 \u0627\u0644\u0639\u0627\u0644\u064a\u0629 (60-80-100%) \u0645\u0642\u0627\u0631\u0646\u0629 \u0628\u0627\u0644\u062a\u0631\u0643\u064a\u0632\u064a\u0646 (20-40%) (\u0627\u0644\u0634\u0643\u0644 2\u060c a). <\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\">\u0628\u064a\u0646\u062a \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062b\u0627\u0646\u0648\u064a\u0629 \u0627\u0644\u0645\u062e\u062a\u0644\u0641\u0629 \u0648\u0627\u0644\u062a\u064a \u0627\u0633\u062a\u062e\u062f\u0645\u062a \u0641\u064a \u0627\u062e\u062a\u0628\u0627\u0631 \u0627\u0644\u062a\u0628\u0635\u064a\u0645 \u0627\u0644\u0645\u0646\u0627\u0639\u064a \u0642\u062f\u0631\u0627\u062a \u0645\u062a\u0641\u0627\u0648\u062a\u0629 \u0639\u0644\u0649 \u0643\u0634\u0641 \u0627\u0644\u0639\u0635\u0627\u0628\u0627\u062a \u0627\u0644\u062e\u0627\u0635\u0629 \u0628\u0643\u0644 \u0645\u0643\u0648\u0646 \u0644\u0645\u0635\u0644 \u0627\u0644\u062c\u0645\u0644 \u0627\u0644\u0645\u0645\u0646\u0639 \u0628\u0633\u0645 \u0627\u0644\u0639\u0642\u0631\u0628\u060c \u0625\u0630 \u0623\u0628\u062f\u062a \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u0623\u0631\u0646\u0628 \u0627\u0644\u0645\u0645\u0646\u0639 \u0628\u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u062b\u0642\u064a\u0644\u0629 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 R-a-IgG 2,3 \u0646\u0648\u0639\u064a\u0629 \u0644\u0643\u0634\u0641 \u0627\u0644\u0633\u0644\u0627\u0633\u0644 \u0627\u0644\u062b\u0642\u064a\u0644\u0629 \u0645\u0646 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062a\u0642\u0644\u064a\u062f\u064a\u0629 \u0623\u0648 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u062b\u0642\u064a\u0644\u0629 \u0627\u0644\u0633\u0644\u0633\u0644\u0629\u060c \u062f\u0648\u0646 \u0623\u0646 \u062a\u0643\u0634\u0641 \u0639\u0646 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u062e\u0641\u064a\u0641\u0629 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 \u0641\u064a \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062a\u0642\u0644\u064a\u062f\u064a\u0629\u060c \u0643\u0630\u0644\u0643 \u0627\u0644\u0623\u0645\u0631 \u0623\u0628\u062f\u062a \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u0623\u0631\u0646\u0628 \u0627\u0644\u0645\u0645\u0646\u0639 \u0628\u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0627\u0644\u0646\u0627\u0646\u0648\u064a\u0629 \u0627\u0644\u062a\u064a \u062a\u0645\u062b\u0644 \u0627\u0644\u0645\u0646\u0637\u0642\u0629 \u0627\u0644\u0645\u062a\u063a\u0627\u064a\u0631\u0629 \u0645\u0646 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u062b\u0642\u064a\u0644\u0629 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 \u0646\u0648\u0639\u064a\u0629 \u0644\u0644\u0643\u0634\u0641 \u0639\u0646 \u0630\u0627\u062a \u0627\u0644\u0633\u0644\u0627\u0633\u0644 \u0627\u0644\u062b\u0642\u064a\u0644\u0629 \u0645\u0639 \u062d\u0633\u0627\u0633\u064a\u0629 \u0623\u0642\u0644. \u0645\u0646 \u0646\u0627\u062d\u064a\u0629 \u0623\u062e\u0631\u0649 \u0623\u0628\u062f\u062a \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u0623\u0631\u0646\u0628 \u0627\u0644\u0645\u0645\u0646\u0639 \u0628\u0645\u0635\u0644 \u0627\u0644\u062c\u0645\u0644 \u0627\u0644\u0643\u0627\u0645\u0644 \u0646\u0648\u0639\u064a\u0629 \u0648\u062d\u0633\u0627\u0633\u064a\u0629 \u0639\u0627\u0644\u064a\u0629 \u0644\u0623\u0644\u0628\u0648\u0645\u064a\u0646 \u0627\u0644\u062c\u0645\u0644 \u0627\u0644\u0645\u0635\u0644\u064a \u062f\u0648\u0646 \u0623\u0646 \u062a\u0628\u062f\u064a \u0623\u064a\u0629 \u0627\u0644\u0641\u0629 \u0644\u063a\u0644\u0648\u0628\u0648\u0644\u064a\u0646\u0627\u062a \u0627\u0644\u062c\u0645\u0644 \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629. \u062a\u0624\u0643\u062f \u0647\u0630\u0647 \u0627\u0644\u0646\u062a\u064a\u062c\u0629 \u0646\u0648\u0639\u064a\u0629 \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u0623\u0631\u0646\u0628 \u0627\u0644\u0645\u0645\u0646\u0639 \u0628\u0627\u0644\u0623\u0636\u062f\u0627\u062f \u062b\u0642\u064a\u0644\u0629 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 R-a-IgG 2,3 \u062a\u062c\u0627\u0647 \u063a\u0644\u0648\u0628\u0648\u0644\u064a\u0646\u0627\u062a \u0627\u0644\u062c\u0645\u0644 IgG (\u0627\u0644\u0634\u0643\u0644 2 b\u060c c\u060c d)\u060c \u0645\u0627 \u064a\u0648\u062c\u0647 \u0646\u062d\u0648 \u0627\u0633\u062a\u062e\u062f\u0627\u0645\u0647\u0627 \u0641\u064a \u0627\u062e\u062a\u0628\u0627\u0631\u0627\u062a \u0627\u0644\u0645\u0642\u0627\u064a\u0633\u0629 \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 \u0627\u0644\u0625\u0646\u0632\u064a\u0645\u064a\u0629 \u0644\u0644\u0645\u0642\u0627\u064a\u0633\u0629 \u0627\u0644\u0646\u0648\u0639\u064a\u0629 \u0644\u0647\u0630\u0647 \u0627\u0644\u0623\u0636\u062f\u0627\u062f.<\/span><\/p>\n<figure id=\"attachment_455\" aria-describedby=\"caption-attachment-455\" style=\"width: 468px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-455\" src=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2022\/10\/22-300x197.jpg\" alt=\"\u0627\u0644\u0634\u0643\u0644 (2) \u0646\u062a\u0627\u0626\u062c \u062a\u0631\u062d\u064a\u0644 \u0627\u0644\u0639\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0631\u0633\u0628\u0629 \u0628\u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0628\u0639\u062f \u0627\u0644\u0635\u0628\u063a \u0628\u0643\u0648\u0645\u0627\u0632\u064a \u0648\u0644\u0637\u062e\u0629 \u0648\u064a\u0633\u062a\u0631\u0646\" width=\"468\" height=\"307\" srcset=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2022\/10\/22-300x197.jpg 300w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2022\/10\/22-768x503.jpg 768w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2022\/10\/22.jpg 873w\" sizes=\"auto, (max-width: 468px) 100vw, 468px\" \/><figcaption id=\"caption-attachment-455\" class=\"wp-caption-text\"><strong><span style=\"font-family: 'times new roman', times, serif;\">\u0627\u0644\u0634\u0643\u0644 (2) \u0646\u062a\u0627\u0626\u062c \u062a\u0631\u062d\u064a\u0644 \u0627\u0644\u0639\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0631\u0633\u0628\u0629 \u0628\u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0628\u0639\u062f \u0627\u0644\u0635\u0628\u063a \u0628\u0643\u0648\u0645\u0627\u0632\u064a \u0648\u0644\u0637\u062e\u0629 \u0648\u064a\u0633\u062a\u0631\u0646<\/span><\/strong><\/figcaption><\/figure>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\"><strong><em><span style=\"font-size: 10pt;\">(a) \u0635\u0628\u063a\u0629 \u0643\u0648\u0645\u0627\u0632\u064a \u0628\u0639\u062f \u062a\u0631\u062d\u064a\u0644 \u0627\u0644\u0639\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0631\u0633\u0628\u0629 \u0628\u0646\u0633\u0628 \u0625\u0634\u0628\u0627\u0639 \u0645\u062a\u0632\u0627\u064a\u062f\u0629 \u0645\u0646 \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0628\u0639\u062f \u0627\u0644\u0648\u0627\u0633\u0645 \u0627\u0644\u0639\u064a\u0627\u0631\u064a (M) \u0645\u0639 \u0634\u0627\u0647\u062f \u0625\u064a\u062c\u0627\u0628\u064a \u064a\u062d\u0648\u064a \u0645\u0632\u064a\u062c \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u0643\u0627\u0645\u0644\u0629 (C1) \u0648 \u0634\u0627\u0647\u062f \u0625\u064a\u062c\u0627\u0628\u064a \u062b\u0627\u0646 \u064a\u062d\u0648\u064a \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u062b\u0642\u064a\u0644\u0629 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 IgG2,3 (C2). \u064a\u0645\u0643\u0646 \u062a\u0645\u064a\u064a\u0632 3 \u0639\u0635\u0627\u0628\u0627\u062a \u0644\u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u0641\u064a \u0627\u0644\u0639\u064a\u0646\u0629 \u0627\u0644\u0645\u0631\u0633\u0628\u0629 \u0628\u062a\u0631\u0643\u064a\u0632 \u0625\u0634\u0628\u0627\u0639\u064a 20% \u0648\u062a\u0645\u062b\u0644 \u0643\u0644 \u0645\u0646 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 \u0627\u0644\u062b\u0642\u064a\u0644\u0629 \u0644\u0644\u0636\u062f \u0627\u0644\u062a\u0642\u0644\u064a\u062f\u064a (HC) \u0648\u0627\u0644\u0633\u0644\u0633\u0644\u0629 \u0627\u0644\u062b\u0642\u064a\u0644\u0629 \u0644\u0644\u0636\u062f \u062b\u0642\u064a\u0644 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 (HHC) \u0648\u0627\u0644\u0633\u0644\u0633\u0629 \u0627\u0644\u062e\u0641\u064a\u0641\u0629 \u0644\u0644\u0636\u062f \u0627\u0644\u062a\u0642\u0644\u064a\u062f\u064a (LC) \u0628\u0634\u0643\u0644 \u0645\u062a\u0648\u0627\u0641\u0642 \u0645\u0639 \u0627\u0644\u0634\u0627\u0647\u062f C1. \u0639\u0646\u062f \u0631\u0641\u0639 \u0627\u0644\u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0625\u0634\u0628\u0627\u0639\u064a \u0625\u0644\u0649 40% \u062a\u0638\u0647\u0631 \u0639\u0635\u0627\u0628\u0629 \u062f\u0627\u0644\u0629 \u0639\u0644\u0649 \u062a\u0631\u0633\u0628 \u0627\u0644\u0645\u0632\u064a\u062f \u0645\u0646 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u062b\u0642\u064a\u0644\u0629 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 \u0628\u0634\u0643\u0644 \u0645\u062a\u0648\u0627\u0641\u0642 \u0645\u0639 \u0627\u0644\u0634\u0627\u0647\u062f \u0627\u0644\u062b\u0627\u0646\u064a C2. (b), (c) \u0648(d) \u0635\u0648\u0631 \u063a\u0634\u0627\u0621 \u0646\u062a\u0631\u0627\u062a \u0627\u0644\u0633\u0644\u064a\u0644\u0648\u0632 \u0628\u0639\u062f \u0627\u0644\u062a\u0628\u0635\u064a\u0645 \u0627\u0644\u0645\u0646\u0627\u0639\u064a \u0627\u0644\u0639\u064a\u0646\u0627\u062a \u0627\u0644\u062e\u0627\u0636\u0639\u0629 \u0644\u0631\u062d\u0644\u0627\u0646 \u0643\u0647\u0631\u0628\u0627\u0626\u064a \u0646\u0641\u0633\u0647\u0627 \u0643\u0645\u0627 \u0641\u064a a \u0648\u0628\u062f\u0644\u0627\u064b \u0639\u0646 \u0627\u0644\u0635\u0628\u063a \u0628\u0643\u0648\u0645\u0627\u0632\u064a \u062a\u0645 \u0646\u0642\u0644 \u0627\u0644\u0639\u064a\u0646\u0627\u062a \u0625\u0644\u0649 \u0627\u0644\u063a\u0634\u0627\u0621 \u0648\u0627\u0644\u0643\u0634\u0641 \u0639\u0646 \u0627\u0644\u0639\u0635\u0627\u0628\u0627\u062a \u0628\u0640\u0625\u0636\u0627\u0641\u0629 3-amino-9-ethylcarbazole (AEC) \u0628\u0648\u0635\u0641\u0647\u0627 \u0631\u0643\u0627\u0632\u0629 \u0644\u0625\u0646\u0632\u064a\u0645 \u0627\u0644\u0628\u064a\u0631\u0648\u0643\u0633\u064a\u062f\u0632 \u0627\u0644\u0645\u062f\u0645\u062c \u0645\u0639 \u0627\u0644\u0636\u062f \u0627\u0644\u0645\u0636\u0627\u0641\u064a \u0641\u064a \u0627\u0644\u062e\u0637\u0648\u0629 \u0627\u0644\u0623\u062e\u064a\u0631\u0629 (1\/3000) G-a-R-HRP. \u0625\u0630 \u0627\u062e\u062a\u0628\u0631 \u0641\u064a (b) R-a-Camel (1\/3000) \u0644\u0644\u0643\u0634\u0641 \u0639\u0646 \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u063a\u064a\u0631 \u0623\u0646 \u0627\u0644\u0639\u0635\u0627\u0628\u0627\u062a \u0627\u0644\u062a\u064a \u0638\u0647\u0631\u062a \u062a\u0645\u062b\u0644 \u0623\u0644\u0623\u0628\u0648\u0645\u064a\u0646 \u0645\u0635\u0644 \u0627\u0644\u062c\u0645\u0644 \u0641\u064a \u062c\u0645\u064a\u0639 \u0645\u0631\u0627\u062d\u0644 \u0627\u0644\u062a\u0631\u0633\u064a\u0628 \u062f\u0648\u0646 \u0638\u0647\u0648\u0631 \u0623\u064a \u0639\u0635\u0627\u0628\u0629 \u0639\u0644\u0649 \u0627\u0644\u0634\u0648\u0627\u0647\u062f \u0627\u0644\u0625\u064a\u062c\u0627\u0628\u064a\u0629\u060c \u0641\u064a (c) \u0627\u062e\u062a\u0628\u0631 R-a-Nb (1\/500) \u0627\u0644\u0630\u064a \u0623\u0638\u0647\u0631 \u0627\u0644\u0639\u0635\u0627\u0628\u0627\u062a \u0627\u0644\u062e\u0627\u0635\u0629 \u0628\u0627\u0644\u0633\u0644\u0627\u0633\u0644 \u0627\u0644\u062b\u0642\u064a\u0644\u0629 \u0644\u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u0627\u0644\u062a\u0642\u0644\u064a\u062f\u064a\u0629 \u0648\u062b\u0642\u064a\u0644\u0629 \u0627\u0644\u0633\u0644\u0633\u0644\u0629. \u0641\u064a (d) R-a-IgG 2,3 (1\/200) \u0623\u0638\u0647\u0631 \u0627\u0644\u0639\u0635\u0627\u0628\u0627\u062a \u0627\u0644\u062e\u0627\u0635\u0629 \u0628\u0627\u0644\u0633\u0644\u0627\u0633\u0644 \u0627\u0644\u062b\u0642\u064a\u0644\u0629 \u0644\u0643\u0627\u0645\u0644 \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644.<\/span><\/em><\/strong><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\"><strong>\u0627\u0644\u0645\u0628\u0627\u062f\u0644\u0629 \u0627\u0644\u062f\u0627\u0631\u0626\u064a\u0629 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0643\u0631\u0648\u0645\u0627\u062a\u0648\u063a\u0631\u0627\u0641\u064a\u0627 \u0627\u0644\u0645\u0628\u0627\u062f\u0644\u0629 \u0627\u0644\u062f\u0627\u0631\u0626\u064a\u0629 <\/strong><strong>\u00a0Buffer exchange chromatography<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\">\u0627\u0639\u062a\u064f\u0645\u062f \u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0625\u0634\u0628\u0627\u0639 40% \u0644\u0644\u062d\u0635\u0648\u0644 \u0639\u0644\u0649 \u0623\u0643\u0628\u0631 \u0645\u0631\u062f\u0648\u062f \u0645\u0645\u0643\u0646 \u0645\u0646 \u0623\u0636\u062f\u0627\u062f IgG \u0644\u0644\u062c\u0645\u0644 \u062f\u0648\u0646 \u062a\u0631\u0633\u064a\u0628 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0627\u0644\u0623\u062e\u0631\u0649 \u0627\u0644\u0645\u0631\u0627\u0641\u0642\u0629 \u0643\u0627\u0644\u0623\u0644\u0628\u0648\u0645\u064a\u0646 \u0628\u0643\u0645\u064a\u0627\u062a \u0643\u0628\u064a\u0631\u0629. \u0628\u0639\u062f \u062a\u0631\u0633\u064a\u0628 \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u0643\u0627\u0646 \u0644\u0627 \u0628\u062f \u0645\u0646 \u0627\u0644\u062a\u062e\u0644\u0635 \u0645\u0646 \u0628\u0642\u0627\u064a\u0627 \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0648\u0627\u0644\u0634\u0648\u0627\u0631\u062f \u0648\u0627\u0644\u0623\u0645\u0644\u0627\u062d \u0627\u0644\u062a\u064a \u0642\u062f \u062a\u0624\u062b\u0631 \u0641\u064a \u0637\u064a \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0648\u0630\u0648\u0628\u0627\u0646\u0647\u060c \u0643\u0645\u0627 \u0623\u0646\u0647\u0627 \u0642\u062f \u062a\u0624\u062b\u0631 \u062a\u0623\u062b\u064a\u0631\u0627\u064b \u0633\u0644\u0628\u064a\u0627\u064b \u0641\u064a \u0623\u0639\u0645\u062f\u0629 \u0627\u0644\u062a\u0646\u0642\u064a\u0629 \u0627\u0644\u0645\u0631\u0627\u062f \u0627\u0633\u062a\u062e\u062f\u0627\u0645\u0647\u0627 \u0641\u064a \u062e\u0637\u0648\u0627\u062a \u062a\u0627\u0644\u064a\u0629. \u0627\u0633\u062a\u062e\u062f\u0645 \u0639\u0645\u0648\u062f HiPrep 26\/10 Desalting\u060c \u062d\u064a\u062b \u062c\u0645\u0639\u062a \u0627\u0644\u0639\u064a\u0646\u0629 \u0627\u0644\u0645\u062d\u062a\u0648\u064a\u0629 \u0639\u0644\u0649 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0648\u0627\u0644\u0645\u0631\u0627\u0641\u0642\u0629 \u0644\u0638\u0647\u0648\u0631 \u0627\u0644\u0642\u0645\u0629 \u0627\u0644\u0623\u0648\u0644\u0649 \u0627\u0644\u0645\u062a\u0639\u0644\u0642\u0629 \u0628\u0627\u0644\u0627\u0645\u062a\u0635\u0627\u0635\u064a\u0629 absorbance \u0641\u064a \u0645\u0648\u062c\u0629 \u0637\u0648\u0644\u0647\u0627 280 \u0646\u0645 \u0648\u0647\u0648 \u0637\u0648\u0644 \u0645\u0648\u062c\u0629 \u0627\u0644\u0642\u064a\u0627\u0633 \u0627\u0644\u062e\u0627\u0635 \u0628\u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0645\u0639 \u0627\u0644\u062f\u0627\u0631\u0626\u0629 \u0627\u0644\u062c\u062f\u064a\u062f\u0629 \u0627\u0644\u0645\u0633\u062a\u062e\u062f\u0645\u0629 \u0644\u0644\u0645\u0628\u0627\u062f\u0644\u0629\u060c \u0641\u064a \u062d\u064a\u0646 \u0644\u0648\u062d\u0638 \u0638\u0647\u0648\u0631 \u0627\u0644\u0642\u0645\u0629 \u0627\u0644\u062e\u0627\u0635\u0629 \u0628\u0627\u0644\u0645\u0643\u0648\u0646\u0627\u062a \u0627\u0644\u0645\u0631\u0627\u0641\u0642\u0629 \u0644\u0644\u0639\u064a\u0646\u0629 \u0645\u0646 \u0634\u0648\u0627\u0631\u062f \u0648\u0623\u0645\u0644\u0627\u062d \u0627\u0633\u062a\u062f\u0644 \u0639\u0644\u064a\u0647\u0627 \u0628\u062a\u0628\u062f\u0644 \u0641\u064a \u0627\u0644\u0646\u0627\u0642\u0644\u064a\u0629 \u0627\u0644\u0643\u0647\u0631\u0628\u0627\u0626\u064a\u0629 \u0645\u0627 \u064a\u0624\u0643\u062f \u0627\u0644\u062a\u062e\u0644\u0635 \u0645\u0646 \u0647\u0630\u0647 \u0627\u0644\u0645\u0643\u0648\u0646\u0627\u062a \u0627\u0644\u062d\u0627\u0648\u064a\u0629 \u0639\u0644\u0649 \u0627\u0644\u0639\u062f\u064a\u062f \u0645\u0646 \u0627\u0644\u0634\u0648\u0627\u0631\u062f \u0627\u0644\u062e\u0627\u0635\u0629 \u0628\u062f\u0627\u0631\u0626\u0629 PBS \u0627\u0644\u0645\u0633\u062a\u062e\u062f\u0645 \u0641\u064a \u062d\u0644 \u0627\u0644\u0639\u064a\u0646\u0627\u062a \u0641\u0636\u0644\u0627\u064b \u0639\u0646 \u0628\u0642\u0627\u064a\u0627 \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0627\u0644\u0634\u0643\u0644 (3\u060c a).<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\"><strong>\u062a\u0646\u0642\u064a\u0629 \u063a\u0644\u0648\u0628\u0648\u0644\u064a\u0646\u0627\u062a \u0627\u0644\u062c\u0645\u0644 \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0643\u0631\u0648\u0645\u0627\u062a\u0648\u063a\u0631\u0627\u0641\u064a\u0627 \u0627\u0644\u0627\u0644\u0641\u0629<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\">\u0623\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u0631\u062d\u0644\u0627\u0646 \u0627\u0644\u0643\u0647\u0631\u0628\u0627\u0626\u064a \u0648\u062c\u0648\u062f \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 \u0639\u0646\u062f \u0627\u0644\u062a\u0631\u0633\u064a\u0628 \u0628\u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0628\u062a\u0631\u0643\u064a\u0632 \u0625\u0634\u0628\u0627\u0639\u064a \u062d\u062a\u0649 40% \u0643\u0645\u0627 \u0623\u0638\u0647\u0631\u062a \u0648\u062c\u0648\u062f \u0623\u0644\u0628\u0648\u0645\u064a\u0646 \u0645\u0635\u0644 \u0627\u0644\u062c\u0645\u0644 \u0641\u064a \u0627\u0644\u0639\u064a\u0646\u0627\u062a \u0627\u0644\u062a\u064a \u0631\u064f\u0633\u0651\u0650\u0628\u062a \u0628\u0647\u0630\u0627 \u0627\u0644\u062a\u0631\u0643\u064a\u0632. \u0648\u0628\u0647\u062f\u0641 \u0627\u0644\u062d\u0635\u0648\u0644 \u0639\u0644\u0649 \u0627\u0644\u063a\u0644\u0648\u0628\u0648\u0644\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 IgG \u0646\u0642\u064a\u0627\u064b \u0627\u0633\u062a\u062e\u062f\u0645 \u0639\u0645\u0648\u062f Protein A \u0630\u0648 \u0627\u0644\u0623\u0644\u0641\u0629 \u0644\u0647\u0630\u0627 \u0627\u0644\u0646\u0648\u0639 \u0645\u0646 \u0627\u0644\u0623\u0636\u062f\u0627\u062f\u060c \u0625\u0630 \u0627\u0631\u062a\u0628\u0637 \u0627\u0644\u062c\u0632\u0621 \u0627\u0644\u0623\u0643\u0628\u0631 \u0645\u0646 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0628\u0627\u0644\u0639\u0645\u0648\u062f \u0641\u064a \u0623\u062b\u0646\u0627\u0621 \u062a\u0645\u0631\u064a\u0631 \u0645\u062d\u0644\u0648\u0644 \u0627\u0644\u0627\u0631\u062a\u0628\u0627\u0637 \u0641\u064a \u062d\u064a\u0646 \u062a\u062f\u0641\u0642\u062a \u0628\u0627\u0642\u064a \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0648\u0638\u0647\u0631\u062a \u0627\u0644\u0642\u0645\u0629 \u0627\u0644\u0623\u0648\u0644\u0649 \u0627\u0644\u0645\u0639\u0628\u0631\u0629 \u0639\u0646 \u0647\u0630\u0627 \u0627\u0644\u062a\u062f\u0641\u0642 Flow throw (Ft)\u060c \u0628\u0639\u062f \u0645\u0631\u0627\u062d\u0644 \u0645\u0633\u062a\u0641\u064a\u0636\u0629 \u0645\u0646 \u0627\u0644\u063a\u0633\u0644 \u0634\u064f\u0637\u0641 \u0627\u0644\u0623\u0636\u062f\u0627\u062f Pure \u0627\u0644\u0645\u0631\u062a\u0628\u0637\u0629 \u0628\u0627\u0644\u0639\u0645\u0648\u062f \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0645\u062d\u0644\u0648\u0644 \u0627\u0644\u0634\u0637\u0641 \u0648\u062c\u0645\u0639\u062a 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\u062a\u0627\u0645 \u0644\u0644\u0639\u0635\u0627\u0628\u0629 \u0627\u0644\u062e\u0627\u0635\u0629 \u0628\u0623\u0644\u0628\u0648\u0645\u064a\u0646 \u0645\u0635\u0644 \u0627\u0644\u062c\u0645\u0644 Alb\u060c \u0641\u064a \u062d\u064a\u0646 \u0644\u0648\u062d\u0638 \u0647\u0631\u0648\u0628 \u062c\u0632\u0621 \u0643\u0628\u064a\u0631 \u0645\u0646 \u0645\u0631\u062f\u0648\u062f \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0625\u0644\u0649 \u062c\u0627\u0646\u0628 \u0627\u0644\u0623\u0644\u0628\u0648\u0645\u064a\u0646 \u0645\u0639 \u0627\u0644\u062a\u062f\u0641\u0642 \u0627\u0644\u062e\u0627\u0635 \u0628\u0627\u0644\u0642\u0645\u0629 \u0627\u0644\u0623\u0648\u0644\u0649 FT \u0645\u0627 \u0642\u062f \u064a\u0641\u0633\u0631 \u0628\u0627\u0644\u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0639\u0627\u0644\u064a \u0644\u0644\u0623\u0636\u062f\u0627\u062f \u0641\u064a \u0627\u0644\u0639\u064a\u0646\u0629 \u0627\u0644\u0645\u0631\u0627\u062f \u062a\u0646\u0642\u064a\u062a\u0647\u0627 \u0627\u0644\u0634\u0643\u0644 (3\u060c b).<\/span><\/p>\n<figure id=\"attachment_456\" aria-describedby=\"caption-attachment-456\" style=\"width: 573px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-456\" src=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2022\/10\/3-1-300x134.jpg\" alt=\"\u0627\u0644\u0634\u0643\u0644 (3) \u062a\u0646\u0642\u064a\u0629 \u063a\u0644\u0648\u0628\u0648\u0644\u064a\u0646\u0627\u062a \u0627\u0644\u062c\u0645\u0644 \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0639\u0645\u0648\u062f \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 A \u0628\u0639\u062f \u0627\u0644\u062a\u062e\u0644\u0635 \u0645\u0646 \u0628\u0642\u0627\u064a\u0627 \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0628\u0627\u0644\u0645\u0628\u0627\u062f\u0644\u0629 \u0627\u0644\u062f\u0627\u0631\u0626\u064a\u0629\" width=\"573\" height=\"256\" srcset=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2022\/10\/3-1-300x134.jpg 300w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2022\/10\/3-1-1024x459.jpg 1024w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2022\/10\/3-1-768x344.jpg 768w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2022\/10\/3-1.jpg 1326w\" sizes=\"auto, (max-width: 573px) 100vw, 573px\" \/><figcaption id=\"caption-attachment-456\" class=\"wp-caption-text\"><strong><span style=\"font-family: 'times new roman', times, serif;\">\u0627\u0644\u0634\u0643\u0644 (3) \u062a\u0646\u0642\u064a\u0629 \u063a\u0644\u0648\u0628\u0648\u0644\u064a\u0646\u0627\u062a \u0627\u0644\u062c\u0645\u0644 \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0639\u0645\u0648\u062f \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 A \u0628\u0639\u062f \u0627\u0644\u062a\u062e\u0644\u0635 \u0645\u0646 \u0628\u0642\u0627\u064a\u0627 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\u0627\u0644\u0623\u0639\u0644\u0649 \u0644\u0644\u0636\u063a\u0637 \u0642\u0628\u0644 \u0648\u0628\u0639\u062f \u0627\u0644\u0639\u0645\u0648\u062f \u0639\u0646\u062f 0.5 \u06480.15 \u0645\u064a\u0644\u064a \u0628\u0627\u0633\u0643\u0627\u0644 \u0639\u0644\u0649 \u0627\u0644\u062a\u0631\u062a\u064a\u0628\u060c \u062d\u0642\u0646\u062a \u0639\u064a\u0646\u0629 \u0628\u062d\u062c\u0645 5 \u0645\u0644 \u0648\u0636\u0628\u0637 \u0645\u0639\u062f\u0644 \u0627\u0644\u062a\u062f\u0641\u0642 \u0641\u064a \u0623\u062b\u0646\u0627\u0621 \u0639\u0645\u0644\u064a\u0629 \u0627\u0644\u062a\u0646\u0642\u064a\u0629 \u0639\u0646\u062f 10 \u0645\u0644\/\u062f. \u062a\u0645\u062b\u0644 \u0627\u0644\u0642\u0645\u0629 \u0627\u0644\u0623\u0648\u0644\u0649 \u0627\u0644\u0627\u0645\u062a\u0635\u0627\u0635\u064a\u0629 \u0639\u0646\u062f 280 \u0646\u0645 \u0645\u0634\u064a\u0631\u0629 \u0625\u0644\u0649 \u062e\u0631\u0648\u062c \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0645\u0639 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\u0628\u0642\u0627\u064a\u0627 \u0627\u0644\u0645\u0643\u0648\u0646\u0627\u062a \u0627\u0644\u0645\u0644\u062d\u064a\u0629 \u0627\u0644\u0645\u0631\u0627\u0641\u0642\u0629 \u0639\u0646\u062f \u0646\u0627\u0642\u0644\u064a\u0629 54 \u0645\u064a\u0644\u064a \u0633\u064a\u0645\u064a\u0646\u0632\/\u0633\u0645\u060c (b) \u064a\u0638\u0647\u0631 \u0627\u0644\u0645\u0633\u062d \u0627\u0644\u0637\u064a\u0641\u064a \u0644\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0639\u0646\u062f \u0637\u0648\u0644 \u0645\u0648\u062c\u0629 280 \u0646\u0645 \u062e\u0644\u0627\u0644 \u0627\u0644\u062a\u0646\u0642\u064a\u0629 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062c\u0647\u0627\u0632 AKTA Pure 150 protein purification system \u0627\u0644\u0645\u0632\u0648\u062f \u0628\u0639\u0645\u0648\u062f protein A. \u062d\u064a\u062b \u0627\u0633\u062a\u062e\u062f\u0645 \u062f\u0627\u0631\u0626\u0629 \u0627\u0644\u0641\u0648\u0633\u0641\u0627\u062a \u0643\u062f\u0627\u0631\u0626\u0629 \u0627\u0631\u062a\u0628\u0627\u0637 binding \u0648\u0627\u0633\u062a\u062e\u062f\u0645 \u0645\u062d\u0644\u0648\u0644 \u062d\u0645\u0636 \u0627\u0644\u0644\u064a\u0645\u0648\u0646 0.1 M\u060c pH= 3 \u0628\u0648\u0635\u0641\u0647 \u0645\u062d\u0644\u0648\u0644 \u0634\u0637\u0641 elution\u060c \u0648\u0636\u0628\u0637 \u0627\u0644\u062d\u062f \u0627\u0644\u0623\u0639\u0644\u0649 \u0644\u0644\u0636\u063a\u0637 \u0642\u0628\u0644 \u0627\u0644\u0639\u0645\u0648\u062f \u0648\u0628\u0639\u062f\u0647 \u0639\u0644\u0649 0.7 \u06480.5 \u0639\u0644\u0649 \u0627\u0644\u062a\u0631\u062a\u064a\u0628\u060c \u0648\u0645\u0639\u062f\u0644 \u0627\u0644\u062a\u062f\u0641\u0642 \u062e\u0644\u0627\u0644 \u0627\u0644\u062a\u062f\u0641\u0642 \u0639\u0628\u0631 \u0627\u0644\u0639\u0645\u0648\u062f \u0639\u0644\u0649 10 \u0645\u0644\/\u062f. \u062d\u0642\u0646\u062a \u0639\u064a\u0646\u0629 \u0628\u062d\u062c\u0645 5 \u0645\u0644 \u0641\u064a \u0623\u062b\u0646\u0627\u0621 \u062a\u0645\u0631\u064a\u0631 \u062f\u0627\u0631\u0626\u0629 \u0627\u0644\u0627\u0631\u062a\u0628\u0627\u0637 \u062d\u064a\u062b \u0638\u0647\u0631\u062a \u0627\u0644\u0642\u0645\u0629 \u0627\u0644\u0623\u0648\u0644\u0649 \u0627\u0644\u062a\u064a \u062a\u0645\u062b\u0644 \u062e\u0631\u0648\u062c \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u063a\u064a\u0631 \u0627\u0644\u0645\u0631\u062a\u0628\u0637\u0629\u060c \u0648\u0645\u0646 \u062b\u0645 \u0645\u0631\u0631 \u0645\u062d\u0644\u0648\u0644 \u0627\u0644\u0634\u0637\u0641 \u0644\u062a\u0638\u0647\u0631 \u0627\u0644\u0642\u0645\u0629 \u0627\u0644\u062b\u0627\u0646\u064a\u0629 \u0627\u0644\u062a\u064a \u062a\u0645\u062b\u0644 \u062e\u0631\u0648\u062c \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0630\u0627\u062a \u0627\u0644\u0627\u0644\u0641\u0629 \u0644\u0644\u0639\u0645\u0648\u062f. (inset) \u062a\u0648\u0636\u062d \u0627\u0644\u0635\u0648\u0631\u0629 \u0627\u0644\u0645\u062f\u062e\u0644\u0629 \u0647\u0644\u0627\u0645\u0629 \u0627\u0644\u0623\u0643\u0631\u064a\u0644 \u0623\u0645\u064a\u062f \u0646\u062a\u064a\u062c\u0629 \u0627\u0644\u0635\u0628\u063a \u0628\u0643\u0648\u0645\u0627\u0632\u064a \u0628\u0639\u062f \u062a\u0631\u062d\u064a\u0644 \u0643\u0644 \u0645\u0646 \u0627\u0644\u0639\u064a\u0646\u0629 \u0627\u0644\u0623\u0633\u0627\u0633\u064a\u0629 \u0627\u0644\u0645\u062d\u0642\u0648\u0646\u0629 \u0642\u0628\u0644 \u0627\u0644\u062a\u0646\u0642\u064a\u0629 (S)\u060c \u0648\u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u062a\u062f\u0641\u0642\u0629 \u062f\u0648\u0646 \u0623\u0646 \u062a\u0631\u062a\u0628\u0637 \u0628\u0627\u0644\u0639\u0645\u0648\u062f flow throw (FT) \u062e\u0644\u0627\u0644 \u062a\u0645\u0631\u064a\u0631 \u062f\u0627\u0631\u0626\u0629 \u0627\u0644\u0627\u0631\u062a\u0628\u0627\u0637 \u062d\u064a\u062b \u062a\u0638\u0647\u0631 \u0639\u0635\u0627\u0628\u0629 \u0627\u0644\u0623\u0644\u0628\u0648\u0645\u064a\u0646 Alb \u0628\u0634\u0643\u0644 \u0623\u0633\u0627\u0633\u064a\u060c \u0648\u0627\u0644\u063a\u0644\u0648\u0628\u0648\u0644\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 \u0627\u0644\u0646\u0642\u064a\u0629 \u0627\u0644\u062a\u064a \u062e\u0631\u062c\u062a \u0645\u0639 \u062f\u0627\u0631\u0626\u0629 \u0627\u0644\u0634\u0637\u0641 (Pure) \u062d\u064a\u062b \u0638\u0647\u0631\u062a \u0627\u0644\u0639\u0635\u0627\u0628\u0627\u062a \u0627\u0644\u062f\u0627\u0644\u0629 \u0639\u0644\u0649 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 \u0627\u0644\u062b\u0642\u064a\u0644\u0629 \u0644\u0644\u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062a\u0642\u0644\u064a\u062f\u064a\u0629 HC \u0648\u0627\u0644\u0639\u0635\u0627\u0628\u0629 \u0627\u0644\u062f\u0627\u0644\u0629 \u0639\u0644\u0649 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 \u0627\u0644\u062b\u0642\u064a\u0644\u0629 \u0644\u0644\u0623\u0636\u062f\u0627\u062f \u062b\u0642\u064a\u0644\u0629 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 HHC\u060c \u0648\u0627\u0644\u0639\u0635\u0627\u0628\u0629 \u0627\u0644\u062f\u0627\u0644\u0629 \u0639\u0644\u0649 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 \u0627\u0644\u062e\u0641\u064a\u0641\u0629 \u0644\u0644\u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062a\u0642\u0644\u064a\u062f\u064a\u0629 LC \u062f\u0648\u0646 \u0623\u064a \u0623\u062b\u0631 \u0644\u0639\u0635\u0627\u0628\u0629 \u0627\u0644\u0623\u0644\u0628\u0648\u0645\u064a\u0646. \u064a\u0638\u0647\u0631 \u0641\u064a \u0627\u0644\u062e\u0637 \u0627\u0644\u0623\u0648\u0644 \u0627\u0644\u0648\u0627\u0633\u0645 \u0627\u0644\u0639\u064a\u0627\u0631\u064a\u00a0 (M) \u0645\u0646 (Vivantis).<\/span><\/em><\/strong><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\"><strong>\u0641\u0635\u0644 \u0623\u0646\u0645\u0627\u0637 \u0627\u0644\u063a\u0644\u0648\u0628\u0648\u0644\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 <\/strong><strong>IgG<\/strong><strong> \u0644\u0644\u062c\u0645\u0644 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062a\u0642\u0646\u064a\u0629 \u0627\u0644\u0627\u0633\u062a\u0628\u0639\u0627\u062f \u0627\u0644\u062d\u062c\u0645\u064a <\/strong><strong>Size exclusion chromatography (SEC)<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\">\u0627\u0633\u062a\u062e\u062f\u0645 \u0639\u0645\u0648\u062f Superdex 200 \u0644\u0641\u0635\u0644 \u0645\u0643\u0648\u0646\u0627\u062a \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u0630\u0627\u062a \u0627\u0644\u0623\u0648\u0632\u0627\u0646 \u0627\u0644\u062c\u0632\u064a\u0626\u064a\u0629 \u0627\u0644\u0645\u062e\u062a\u0644\u0641\u0629 \u0628\u062a\u0642\u0646\u064a\u0629 \u0627\u0644\u0627\u0633\u062a\u0628\u0639\u0627\u062f \u0627\u0644\u062d\u062c\u0645\u064a\u060c \u0644\u0648\u062d\u0638 \u0641\u064a \u0627\u0644\u0643\u0631\u0648\u0645\u0627\u062a\u0648\u063a\u0631\u0627\u0645 \u0638\u0647\u0648\u0631 \u062b\u0644\u0627\u062b \u0642\u0645\u0645 \u0645\u062a\u062a\u0627\u0644\u064a\u0629\u060c \u0628\u0639\u062f \u062a\u0631\u062d\u064a\u0644 5 \u0645\u0643\u063a \u0645\u0646 \u0643\u0644 \u0645\u0646 \u0627\u0644\u0639\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u062c\u0645\u0648\u0639\u0629 \u0639\u0646\u062f \u0643\u0644 \u0642\u0645\u0629 \u0648\u0635\u0628\u063a \u0647\u0644\u0627\u0645\u0629 \u0627\u0644\u0623\u0643\u0631\u064a\u0644\u0627\u0645\u064a\u062f \u0628\u0623\u0632\u0631\u0642 \u0627\u0644\u0643\u0648\u0645\u0627\u0632\u064a \u062a\u0628\u064a\u0646 \u0623\u0646 \u0627\u0644\u0642\u0645\u0629 \u0627\u0644\u0623\u0648\u0644\u0649 \u062a\u0645\u062b\u0644 \u0641\u0627\u0626\u0636 \u0645\u0646 \u0627\u0644\u0639\u064a\u0646\u0629 \u064a\u062d\u0648\u064a \u0645\u0632\u064a\u062c \u0645\u0643\u0648\u0646\u0627\u062a \u0645\u0645\u0627\u062b\u0644\u0629 \u0644\u0644\u0639\u064a\u0646\u0629 \u0627\u0644\u0623\u0633\u0627\u0633\u064a\u0629 \u0627\u0644\u0645\u062d\u0642\u0648\u0646\u0629 \u0636\u0645\u0646 \u0627\u0644\u0639\u0645\u0648\u062f\u060c \u0641\u064a \u062d\u064a\u0646 \u0643\u0627\u0646 \u0645\u0631\u062f\u0648\u062f \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062a\u0642\u0644\u064a\u062f\u064a\u0629 \u0623\u0643\u0628\u0631 \u0645\u0646 \u0645\u0631\u062f\u0648\u062f \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u062b\u0642\u064a\u0644\u0629 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 \u0641\u064a \u0627\u0644\u0642\u0645\u0629 \u0627\u0644\u062b\u0627\u0646\u064a\u0629\u060c \u0648\u0638\u0647\u0631\u062a \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u062b\u0642\u064a\u0644\u0629 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 \u0646\u0642\u064a\u0629\u064b \u0641\u064a \u0627\u0644\u0642\u0645\u0629 \u0627\u0644\u062b\u0627\u0644\u062b\u0629 \u0645\u0627 \u064a\u0624\u0643\u062f \u0643\u0641\u0627\u0621\u0629 \u0639\u0645\u0644\u064a\u0629 \u0627\u0644\u0641\u0635\u0644 \u0628\u062a\u0642\u0646\u064a\u0629 \u0627\u0644\u0627\u0633\u062a\u0628\u0639\u0627\u062f \u0627\u0644\u062d\u062c\u0645\u064a \u0627\u0644\u062a\u064a \u0623\u0633\u0647\u0645\u062a \u0641\u064a \u062a\u0646\u0642\u064a\u0629 \u0647\u0630\u0627 \u0627\u0644\u0646\u0648\u0639 \u0627\u0644\u0645\u0645\u064a\u0632 \u0645\u0646 \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 (\u0627\u0644\u0634\u0643\u0644 4).<\/span><\/p>\n<figure id=\"attachment_457\" aria-describedby=\"caption-attachment-457\" style=\"width: 429px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-457\" src=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2022\/10\/4-1-300x182.jpg\" alt=\"\u0627\u0644\u0634\u0643\u0644 (4) \u0641\u0635\u0644 \u0623\u0646\u0645\u0627\u0637 \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0639\u0645\u0648\u062f superdex 200\" width=\"429\" height=\"260\" srcset=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2022\/10\/4-1-300x182.jpg 300w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2022\/10\/4-1.jpg 713w\" sizes=\"auto, (max-width: 429px) 100vw, 429px\" \/><figcaption id=\"caption-attachment-457\" class=\"wp-caption-text\"><strong><span style=\"font-family: 'times new roman', times, serif;\">\u0627\u0644\u0634\u0643\u0644 (4) \u0641\u0635\u0644 \u0623\u0646\u0645\u0627\u0637 \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0639\u0645\u0648\u062f superdex 200<\/span><\/strong><\/figcaption><\/figure>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\"><em><strong><span style=\"font-size: 10pt;\">\u062c\u0631\u0649 \u062d\u0642\u0646 0.5 \u0645\u0644 \u0645\u0646 \u0627\u0644\u0639\u064a\u0646\u0629 \u0627\u0644\u0646\u0627\u062a\u062c\u0629 \u0639\u0646 \u0627\u0644\u062a\u0646\u0642\u064a\u0629 \u0628\u0639\u0645\u0648\u062f \u0628\u0631\u0648\u062a\u064a\u0646 A \u0628\u0639\u062f \u0623\u0646 \u062c\u0631\u0649 \u062a\u0631\u0643\u064a\u0632\u0647\u0627 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 vivaspin 10000 kDa \u0644\u064a\u0635\u0628\u062d \u062a\u0631\u0643\u064a\u0632\u0647\u0627 \u2248 20 \u0645\u063a\/\u0645\u0644\u060c \u062d\u064a\u062b \u0645\u0631\u0631\u062a \u0642\u0628\u0644 \u0627\u0644\u062d\u0642\u0646 \u0639\u0644\u0649 \u0641\u0644\u062a\u0631 0.22 \u0645\u0643\u0645\u060c \u0645\u0631\u0631 \u0627\u0644\u0645\u0627\u0621 \u0628\u0645\u062f\u0629 \u0633\u0627\u0639\u062a\u064a\u0646 \u0628\u062a\u062f\u0641\u0642 1 \u0645\u0644\/\u062f\u060c \u0648\u0645\u0646 \u062b\u0645 \u0645\u0631\u0631\u062a \u062f\u0627\u0631\u0626\u0629 \u0627\u0644\u063a\u0633\u0644 washing buffer \u0623\u064a\u0636\u0627 \u0644\u0645\u062f\u0629 \u0633\u0627\u0639\u062a\u064a\u0646 \u0648\u0645\u0646 \u062b\u0645 \u062d\u0642\u0646\u062a \u0627\u0644\u0639\u064a\u0646\u0629 \u0648\u062c\u0645\u0639\u062a \u0627\u0644\u0639\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0631\u0627\u0641\u0642\u0629 \u0644\u0638\u0647\u0648\u0631 \u0643\u0644 \u0642\u0645\u0629. (inset) \u062a\u0628\u064a\u0646 \u0627\u0644\u0635\u0648\u0631\u0629 \u0627\u0644\u0645\u062f\u062e\u0644\u0629 \u0646\u062a\u064a\u062c\u0629 \u0627\u0644\u0635\u0628\u063a \u0628\u0643\u0648\u0645\u0627\u0632\u064a \u0628\u0639\u062f \u062a\u0631\u062d\u064a\u0644 \u0645\u0627 \u064a\u0639\u0627\u062f\u0644 5 \u0645\u0643\u063a \u0645\u0646 \u0643\u0644 \u0639\u064a\u0646\u0629 \u0646\u0627\u062a\u062c\u0629 \u0639\u0646 \u0627\u0644\u0642\u0645\u0645 (1\u060c 2\u060c 3) \u0625\u0644\u0649 \u062c\u0627\u0646\u0628 \u0627\u0644\u0648\u0627\u0633\u0645 \u0627\u0644\u0639\u064a\u0627\u0631\u064a\u060c \u0625\u0630 \u062a\u0645\u062b\u0644 \u0627\u0644\u0642\u0645\u0629 \u0627\u0644\u0623\u0648\u0644\u0649 (1) \u0645\u0643\u0648\u0646\u0627\u062a \u0627\u0644\u0639\u064a\u0646\u0629 \u0627\u0644\u0623\u0633\u0627\u0633\u064a\u0629 \u0627\u0644\u062a\u064a \u0638\u0647\u0631\u062a \u0645\u0639\u0628\u0631\u0629 \u0639\u0646 \u0641\u0627\u0626\u0636 \u0641\u064a \u062a\u0631\u0643\u064a\u0632 \u0627\u0644\u0639\u064a\u0646\u0629\u060c \u0625\u0630 \u062a\u062d\u062a\u0648\u064a \u0627\u0644\u0639\u0635\u0627\u0628\u0627\u062a \u0627\u0644\u062f\u0627\u0644\u0629 \u0639\u0644\u0649 \u0643\u0627\u0641\u0629 \u0623\u0646\u0645\u0627\u0637 \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644\u060c \u0641\u064a \u062d\u064a\u0646 \u064a\u0644\u0627\u062d\u0638 \u0641\u064a \u0627\u0644\u0642\u0645\u0629 \u0627\u0644\u062b\u0627\u0646\u064a\u0629 (2) \u0638\u0647\u0648\u0631 \u0627\u0644\u0639\u0635\u0627\u0628\u062a\u064a\u0646 \u0627\u0644\u062f\u0627\u0644\u062a\u064a\u0646 \u0639\u0644\u0649 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 \u0627\u0644\u062b\u0642\u064a\u0644\u0629 \u0648\u0627\u0644\u062e\u0641\u064a\u0641\u0629 \u0644\u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u0627\u0644\u062a\u0642\u0644\u064a\u062f\u064a\u0629 \u062a\u0642\u0631\u064a\u0628\u0627\u064b \u0639\u0646\u062f 60 \u064827 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\u0643\u064a\u0644\u0648\u062f\u0627\u0644\u062a\u0648\u0646.<\/span><\/strong><\/em><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\"><strong>\u0627\u062e\u062a\u0628\u0627\u0631 \u062b\u0628\u0627\u062a\u064a\u0629 \u0623\u0646\u0645\u0627\u0637 \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u062a\u062c\u0627\u0647 \u0627\u0644\u062d\u0631\u0627\u0631\u0629 \u0648\u0627\u0644\u0623\u0643\u0633\u062f\u0629 <\/strong><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\">\u0623\u0628\u062f\u062a \u0623\u0646\u0645\u0627\u0637 \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u0627\u0644\u0645\u062e\u062a\u0644\u0641\u0629 \u0642\u062f\u0631\u0629 \u0643\u0644 \u0645\u0646\u0647\u0627 \u0639\u0644\u0649 \u062a\u0639\u0631\u0641 \u0627\u0644\u0645\u0633\u062a\u0636\u062f \u0627\u0644\u0646\u0648\u0639\u064a (\u0633\u0645 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id=\"attachment_458\" aria-describedby=\"caption-attachment-458\" style=\"width: 447px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-458\" src=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2022\/10\/555-300x239.jpg\" alt=\"\u0627\u0644\u0634\u0643\u0644 (5) \u0627\u0644\u0645\u0642\u0627\u064a\u0633\u0629 \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 \u0627\u0644\u0625\u0646\u0632\u064a\u0645\u064a\u0629 \u0644\u0639\u064a\u0646\u0627\u062a \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u0627\u0644\u0645\u0639\u0631\u0636\u0629 \u0644\u0634\u0631\u0648\u0637 \u062a\u062e\u0631\u064a\u0628 \u0642\u0633\u0631\u064a\" width=\"447\" height=\"356\" srcset=\"https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2022\/10\/555-300x239.jpg 300w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2022\/10\/555-768x613.jpg 768w, https:\/\/pharmjournal.asscph.com\/wp-content\/uploads\/2022\/10\/555.jpg 846w\" sizes=\"auto, (max-width: 447px) 100vw, 447px\" \/><figcaption id=\"caption-attachment-458\" class=\"wp-caption-text\"><strong><span style=\"font-family: 'times new roman', times, serif;\">\u0627\u0644\u0634\u0643\u0644 (5) \u0627\u0644\u0645\u0642\u0627\u064a\u0633\u0629 \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 \u0627\u0644\u0625\u0646\u0632\u064a\u0645\u064a\u0629 \u0644\u0639\u064a\u0646\u0627\u062a \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u0627\u0644\u0645\u0639\u0631\u0636\u0629 \u0644\u0634\u0631\u0648\u0637 \u062a\u062e\u0631\u064a\u0628 \u0642\u0633\u0631\u064a<\/span><\/strong><\/figcaption><\/figure>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\">\u064a\u0648\u0636\u062d \u0627\u0644\u0634\u0643\u0644 \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u0645\u0642\u0627\u064a\u0633\u0629 \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 \u0627\u0644\u0625\u0646\u0632\u064a\u0645\u064a\u0629 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\u0627\u0644\u0628\u064a\u0631\u0648\u0643\u0633\u064a\u062f\u0627\u0632G-a-R-HRP \u060c \u0648\u0627\u0633\u062a\u062e\u062f\u0645\u062a \u0631\u0643\u0627\u0632\u0629 TMP \u0627\u0644\u062e\u0627\u0635\u0629 \u0628\u0625\u0646\u0632\u064a\u0645 \u0627\u0644\u0628\u064a\u0631\u0648\u0643\u0633\u064a\u062f\u0627\u0632 \u0641\u064a \u0627\u0644\u062e\u0637\u0648\u0629 \u0627\u0644\u0623\u062e\u064a\u0631\u0629 \u062b\u0645 \u0623\u0636\u064a\u0641 \u062d\u0645\u0636 \u0627\u0644\u0643\u0628\u0631\u064a\u062a 0.5 \u0645\u0648\u0644\/\u0644\u062a\u0631 \u0644\u0625\u0646\u0647\u0627\u0621 \u0627\u0644\u062a\u0641\u0627\u0639\u0644 \u0648\u0642\u0631\u0626\u062a \u0627\u0644\u0627\u0645\u062a\u0635\u0627\u0635\u064a\u0629 \u0641\u064a \u0645\u0648\u062c\u0629 \u0637\u0648\u0644\u0647\u0627 450 \u0646\u0645.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\"><strong>\u0627\u0644\u0645\u0646\u0627\u0642\u0634\u0629 <\/strong><strong>Discussion<\/strong><strong>:<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\">\u062a\u062e\u062a\u0644\u0641 \u0646\u0633\u0628\u0629 \u0625\u0634\u0628\u0627\u0639 \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0627\u0644\u0645\u0633\u062a\u062e\u062f\u0645\u0629 \u0641\u064a \u0623\u062b\u0646\u0627\u0621 \u0641\u0635\u0644 \u0627\u0644\u0645\u0635\u0644 \u0625\u0644\u0649 \u0645\u0643\u0648\u0646\u0627\u062a\u0647 Fractionation \u0644\u062a\u0631\u0633\u064a\u0628 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0628\u064a\u0646 \u0646\u0648\u0639 \u062d\u064a\u0648\u0627\u0646 \u062a\u062c\u0631\u0628\u0629 \u0648\u0622\u062e\u0631\u060c \u0648\u0639\u0627\u062f\u0629 \u0645\u0627 \u064a\u062a\u0645 \u062a\u062d\u062f\u064a\u062f \u0646\u0633\u0628\u0629 \u0627\u0644\u0625\u0634\u0628\u0627\u0639 \u0627\u0644\u0623\u0645\u062b\u0644 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\u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u062a\u064a \u0647\u062f\u0641\u062a \u0625\u0644\u0649 \u0639\u0632\u0644 \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u0627\u0644\u0645\u0645\u0646\u0639 \u0628\u0633\u0645 \u0623\u0641\u0639\u0649 \u0645\u0646 \u0646\u0648\u0639 Naja Naja Oxiana \u0644\u063a\u0627\u064a\u0627\u062a \u0639\u0644\u0627\u062c\u064a\u0629 \u0623\u064a\u0636\u0627\u064b\u060c \u062d\u064a\u062b \u0648\u062c\u062f \u0627\u0644\u0628\u0627\u062d\u062b\u0648\u0646 \u0641\u064a \u0647\u0630\u0647 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u0623\u0646 \u0646\u0633\u0628\u0629 \u0625\u0634\u0628\u0627\u0639 \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 55% \u0647\u064a \u0627\u0644\u0623\u0645\u062b\u0644 \u0644\u0644\u062d\u0635\u0648\u0644 \u0639\u0644\u0649 \u0623\u0639\u0644\u0649 \u0645\u0631\u062f\u0648\u062f \u0645\u0646 \u0627\u0644\u0623\u0636\u062f\u0627\u062f IgG \u0641\u064a \u0645\u0635\u0644 \u0627\u0644\u062c\u0645\u0644. \u062d\u064a\u062b \u062a\u0627\u0628\u0639\u062a \u0647\u0630\u0647 \u0627\u0644\u062f\u0631\u0627\u0633\u0629 \u062a\u0646\u0642\u064a\u0629 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0643\u0631\u0648\u0645\u0627\u062a\u0648\u063a\u0631\u0627\u0641\u064a\u0627 \u0627\u0644\u0645\u0628\u0627\u062f\u0644\u0629 \u0627\u0644\u0634\u0627\u0631\u062f\u064a\u0629 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0639\u0645\u0648\u062f DEAE-Sepharose CL-6B \u0648\u0641\u0642\u0627 \u0644\u0646\u0642\u0637\u0629 \u0627\u0644\u062a\u0639\u0627\u062f\u0644 \u0627\u0644\u0643\u0647\u0631\u0628\u0627\u0626\u064a \u0644\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0644\u0647\u0630\u0647 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0648\u0623\u062b\u0628\u062a\u062a \u0628\u0639\u062f \u0627\u0644\u0645\u0642\u0627\u0631\u0646\u0629 \u0628\u0623\u0636\u062f\u0627\u062f 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\u0646\u0648\u0639\u064a\u062a\u0647 \u0648\u0644\u0623\u0644\u0641\u0629 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0644\u0647 \u0648\u0633\u0647\u0648\u0644\u0629 \u0634\u0637\u0641\u0647\u0627 \u0628\u0639\u062f \u0627\u0631\u062a\u0628\u0627\u0637\u0647\u0627 \u0628\u0627\u0644\u0639\u0645\u0648\u062f. \u0641\u0635\u0644\u062a \u0646\u064f\u0645\u064a\u0637\u0627\u062a \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 IgG \u0641\u064a \u062f\u0631\u0627\u0633\u0627\u062a \u0633\u0627\u0628\u0642\u0629 \u0628\u0648\u0627\u0633\u0637\u0629 \u0627\u0644\u062a\u0646\u0642\u064a\u0629 \u0627\u0644\u062a\u0641\u0627\u0636\u0644\u064a\u0629 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0639\u0645\u0648\u062f\u064a \u0628\u0631\u0648\u062a\u064a\u0646 A \u0648\u0628\u0631\u0648\u062a\u064a\u0646 G \u0628\u0639\u062f\u0629 \u062e\u0637\u0648\u0627\u062a [6, 17]. \u0628\u0639\u062f \u0627\u0644\u062d\u0635\u0648\u0644 \u0639\u0644\u0649 \u0645\u0631\u062f\u0648\u062f 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\u0641\u064a \u0627\u0644\u0635\u064a\u0627\u063a\u0629 \u0627\u0644\u0635\u064a\u062f\u0644\u0627\u0646\u064a\u0629 \u0644\u0647\u0630\u0647 \u0627\u0644\u0623\u0636\u062f\u0627\u062f\u060c \u0644\u0627 \u0633\u064a\u0645\u0627 \u0623\u0646 \u0641\u0639\u0627\u0644\u064a\u0629 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0639\u0645\u0648\u0645\u0627\u064b \u0645\u062a\u0639\u0644\u0642\u0629 \u0628\u0648\u062c\u0648\u062f\u0647\u0627 \u0628\u0634\u0643\u0644\u0647\u0627 \u0627\u0644\u0645\u0637\u0648\u064a \u0645\u062d\u0627\u0641\u0638\u0629 \u0639\u0644\u0649 \u0627\u0644\u0645\u0633\u062a\u0648\u0649 \u0627\u0644\u0631\u0627\u0628\u0639 \u0645\u0646 \u0628\u0646\u064a\u062a\u0647\u0627\u060c \u0648\u0631\u063a\u0645 \u0623\u0646 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u062a\u0639\u062f \u0645\u0646 \u0623\u0643\u062b\u0631 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u062b\u0628\u0627\u062a\u0627\u064b \u0625\u0644\u0627 \u0623\u0646 \u0641\u0639\u0627\u0644\u064a\u062a\u0647\u0627 \u0645\u0631\u062a\u0628\u0637\u0629 \u0628\u0627\u0644\u062c\u0632\u0621 \u0627\u0644\u0645\u062a\u063a\u0627\u064a\u0631 variable \u0648\u0628\u0634\u0643\u0644 \u0623\u0643\u062b\u0631 \u062a\u062d\u062f\u064a\u062f\u0627\u064b \u0628\u0627\u0644\u0639\u0631\u0649 \u0641\u064a \u0627\u0644\u0645\u0646\u0627\u0637\u0642 \u0627\u0644\u0645\u062d\u062f\u062f\u0629 \u0644\u0644\u0645\u062a\u0645\u0645\u0629 Complementarity determining regions (CDRs)\u060c \u062d\u064a\u062b \u0625\u0646 \u0623\u064a \u062a\u063a\u064a\u0631 \u0641\u064a \u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u0623\u062d\u0645\u0627\u0636 \u0627\u0644\u0623\u0645\u064a\u0646\u064a\u0629 \u0639\u0644\u0649 \u0647\u0630\u0647 \u0627\u0644\u0645\u0646\u0627\u0637\u0642 \u0633\u064a\u0624\u062b\u0631 \u062a\u0623\u062b\u064a\u0631\u0627\u064b \u0645\u0628\u0627\u0634\u0631\u0627\u064b \u0634\u0643\u0644 \u0627\u0644\u0633\u0637\u062d \u0641\u064a \u0627\u0644\u0645\u0646\u0637\u0642\u0629 \u0627\u0644\u0645\u062a\u063a\u0627\u064a\u0631\u0629 \u0648\u0645\u0627 \u064a\u0644\u064a\u0647 \u0645\u0646 \u0646\u0642\u0635 \u0641\u064a \u0627\u0644\u0646\u0648\u0639\u064a\u0629 \u0648\u0627\u0644\u0642\u062f\u0631\u0629 \u0639\u0644\u0649 \u0627\u0644\u0627\u0631\u062a\u0628\u0627\u0637 \u0648\u062a\u0639\u0631\u0641 \u0627\u0644\u0645\u0633\u062a\u0636\u062f [23]. \u0623\u0643\u062f\u062a \u0647\u0630\u0647 \u0627\u0644\u0645\u0642\u0627\u0631\u0646\u0629 \u0627\u0646\u0639\u062f\u0627\u0645 \u0641\u0648\u0627\u0631\u0642 \u064a\u0639\u062a\u062f \u0628\u0647\u0627 \u0628\u064a\u0646 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u062b\u0642\u064a\u0644\u0629 \u0627\u0644\u0633\u0644\u0633\u0644\u0629 \u0648\u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062a\u0642\u0644\u064a\u062f\u064a\u0629 \u0648\u0627\u0644\u0645\u0632\u064a\u062c \u0627\u0644\u0643\u0627\u0645\u0644 \u0644\u0647\u0630\u0647 \u0627\u0644\u0623\u0636\u062f\u0627\u062f\u060c \u0645\u0627 \u064a\u062a\u064a\u062d \u0627\u0644\u0627\u0633\u062a\u0641\u0627\u062f\u0629 \u0645\u0646 \u0627\u0644\u0645\u0631\u062f\u0648\u062f \u0627\u0644\u0643\u0627\u0645\u0644 \u0644\u0644\u0623\u0636\u062f\u0627\u062f \u0641\u064a \u062a\u062d\u0636\u064a\u0631 \u0645\u0646\u062a\u062c \u0635\u064a\u062f\u0644\u0627\u0646\u064a \u062a\u0631\u064a\u0627\u0642\u0627\u064b \u0644\u0639\u0644\u0627\u062c \u0644\u062f\u063a\u0629 \u0627\u0644\u0639\u0642\u0631\u0628.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\"><strong>\u00a0<\/strong><strong>\u0627\u0644\u0627\u0633\u062a\u0646\u062a\u0627\u062c\u0627\u062a Conclusions:<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'times new roman', times, serif;\">\u0637\u0648\u0631\u062a\u00a0 \u0637\u0631\u064a\u0642\u0629 \u0644\u062a\u0631\u0633\u064a\u0628 \u0643\u0627\u0641\u0629 \u0623\u0636\u062f\u0627\u062f \u0627\u0633\u0645 \u0627\u0644\u0639\u0642\u0631\u0628 \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0645\u062d\u0644\u0648\u0644 \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0630\u0648 \u062a\u0631\u0643\u064a\u0632 \u0625\u0634\u0628\u0627\u0639\u064a \u0646\u0647\u0627\u0626\u064a 40%\u060c \u0648\u0645\u0646 \u062b\u0645 \u062d\u064f\u0644\u0651\u064e \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646 \u0648\u0627\u0644\u062a\u062e\u0644\u0635 \u0645\u0646 \u0628\u0642\u0627\u064a\u0627 \u0633\u0644\u0641\u0627\u062a \u0627\u0644\u0623\u0645\u0648\u0646\u064a\u0648\u0645 \u0628\u0627\u0644\u0645\u0628\u0627\u062f\u0644\u0629 \u0627\u0644\u062f\u0627\u0631\u0626\u064a\u0629 \u0644\u064a\u062a\u062e\u0644\u0635 \u0641\u064a \u062e\u0637\u0648\u0629 \u0644\u0627\u062d\u0642\u0629 \u0645\u0646 \u062c\u0645\u064a\u0639 \u0627\u0644\u0628\u0631\u0648\u062a\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0635\u0644\u064a\u0629 \u063a\u064a\u0631 \u0627\u0644\u0645\u0631\u063a\u0648\u0628\u0629 \u0645\u0646 \u062e\u0644\u0627\u0644 \u062a\u0646\u0642\u064a\u0629 \u0623\u0636\u062f\u0627\u062f \u0633\u0645 \u0627\u0644\u0639\u0642\u0631\u0628\u060c \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u0639\u0645\u0648\u062f \u0628\u0631\u0648\u062a\u064a\u0646 A \u0630\u0627\u062a \u0627\u0644\u0623\u0644\u0641\u0629 \u0644\u0647\u0630\u0647 \u0627\u0644\u0623\u0636\u062f\u0627\u062f\u060c \u0648\u0628\u0630\u0644\u0643 \u062d\u0635\u0644\u0646\u0627 \u0639\u0644\u0649 \u0645\u0631\u062f\u0648\u062f \u0639\u0627\u0644 \u0645\u0646 \u0623\u0636\u062f\u0627\u062f \u0627\u0644\u062c\u0645\u0644 \u0627\u0644\u0646\u0648\u0639\u064a \u0644\u0633\u0645 \u0627\u0644\u0639\u0642\u0631\u0628 \u0628\u0645\u0627 \u064a\u062e\u062f\u0645 \u0623\u0647\u062f\u0627\u0641 \u062f\u0631\u0627\u0633\u0629 \u0627\u0644\u0635\u064a\u0627\u063a\u0629 \u0627\u0644\u0635\u064a\u062f\u0644\u0627\u0646\u064a\u0629. \u0623\u062b\u0628\u062a\u062a \u0623\u0646\u0645\u0627\u0637 \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0641\u0639\u0627\u0644\u064a\u062a\u0647\u0627 \u0641\u064a \u0627\u0644\u0627\u0631\u062a\u0628\u0627\u0637 \u0628\u0627\u0644\u0645\u0633\u062a\u0636\u062f \u0639\u0644\u0649 \u0635\u0641\u064a\u062d\u0629 \u0627\u0644\u0645\u0642\u0627\u064a\u0633\u0629 \u0627\u0644\u0645\u0646\u0627\u0639\u064a\u0629 \u0627\u0644\u0625\u0646\u0632\u064a\u0645\u064a\u0629\u060c \u0643\u0645\u0627 \u0623\u0638\u0647\u0631\u062a \u0635\u0641\u0627\u062a \u062b\u0628\u0627\u062a \u0645\u062a\u0634\u0627\u0628\u0647\u0629 \u062a\u062c\u0627\u0647 \u0634\u0631\u0648\u0637 \u0627\u0644\u062a\u062e\u0631\u064a\u0628 \u0627\u0644\u0642\u0633\u0631\u064a \u0628\u0627\u0644\u062d\u0631\u0627\u0631\u0629 \u0648\u0627\u0644\u0623\u0643\u0633\u062f\u0629\u060c \u0648\u0647\u0630\u0627 \u064a\u0624\u0643\u062f \u0623\u0647\u0645\u064a\u0629 \u0627\u0644\u0627\u0633\u062a\u0641\u0627\u062f\u0629 \u0645\u0646 \u0643\u0627\u0645\u0644 \u0645\u0631\u062f\u0648\u062f \u0627\u0644\u0623\u0636\u062f\u0627\u062f \u0641\u064a \u0627\u0644\u062f\u0631\u0627\u0633\u0627\u062a \u0627\u0644\u0635\u064a\u062f\u0644\u0627\u0646\u064a\u0629<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u0627\u0644\u0645\u0642\u062f\u0645\u0629 Introduction: \u064a\u0639\u062f \u0627\u0644\u062a\u0633\u0645\u0645 \u0628\u0644\u062f\u063a\u0629 \u0627\u0644\u0639\u0642\u0631\u0628 \u0645\u0646 \u0645\u0634\u0627\u0643\u0644 \u0627\u0644\u0635\u062d\u0629 \u0627\u0644\u0639\u0627\u0645\u0629 \u0627\u0644\u062e\u0637\u064a\u0631\u0629 \u0641\u064a \u0627\u0644\u0639\u062f\u064a\u062f \u0645\u0646 \u0628\u0644\u062f\u0627\u0646 \u0627\u0644\u0639\u0627\u0644\u0645. \u064a\u0641\u0635\u062d \u0633\u0646\u0648\u064a\u0627 \u0639\u0645\u0651\u0627 \u064a\u0642\u0627\u0631\u0628 100000 \u062d\u0627\u062f\u062b\u0629&#8230; <br \/><a href=\"https:\/\/pharmjournal.asscph.com\/?p=87\">\u0627\u0642\u0631\u0623 \u0627\u0644\u0645\u0632\u064a\u062f<\/a><\/p>\n","protected":false},"author":1,"featured_media":457,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[136],"tags":[],"class_list":["post-87","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-136","issue-134","volume-9"],"acf":[],"_links":{"self":[{"href":"https:\/\/pharmjournal.asscph.com\/index.php?rest_route=\/wp\/v2\/posts\/87","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pharmjournal.asscph.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pharmjournal.asscph.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pharmjournal.asscph.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/pharmjournal.asscph.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=87"}],"version-history":[{"count":0,"href":"https:\/\/pharmjournal.asscph.com\/index.php?rest_route=\/wp\/v2\/posts\/87\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pharmjournal.asscph.com\/index.php?rest_route=\/wp\/v2\/media\/457"}],"wp:attachment":[{"href":"https:\/\/pharmjournal.asscph.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=87"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pharmjournal.asscph.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=87"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pharmjournal.asscph.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=87"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}